AN ELECTRONIC EXCEL-CALCULATOR FOR CALCULATING DENTAL CROWN VOLUMES

Author(s):  
O. I. Bulbuk ◽  
V. V. Mazurenko ◽  
M. M. Rozhko ◽  
O. V. Bulbuk

Previous studies have found that with increasing volume of hard tissues defect, the sensitivity of the restored structure to the physical and mechanical restoration characteristics is increasing too. Since existing volume determination techniques cannot be use in a clinical setting, this gives reason to consider the problem of volume determination dental hard tissue defects extremely important, and this research-relevant. Aims of research. Develop and evaluate the possibility of using methods to determine dental crown volumes and volumes of defects in hard tooth tissue. Materials and methods of research. For determination of crown volumes and defect volumes of hard tooth tissues for each group of teeth were used appropriate geometric models. Together with the built models, were performed the mathematical calculations to calculate their linear dimensions. Results of the research. We have developed and substantiated the feasibility of using an electronic Excel calculator to calculate dental crown volumes in clinical settings. Conclusions. Using our electronic Excel calculator to calculate tooth crown volumes and methods for determining the volume of dental hard tissue defects, in clinical settings we can objectify the diagnostic process in the presence of dental hard tissue defects.

2016 ◽  
Vol 23 (4) ◽  
pp. 201642
Author(s):  
Olena Bulbuk ◽  
Mykola Rozhko

The selection of an optimal treatment is one of the problems when restoring the defects of dental hard tissues after endodontic treatment. The work aims at studying the percentage ratio of the volume of the coronal portion of the tooth to its crown that will allow us to optimize the determination of the extent of dental hard tissue defect after endodontic treatment as well as to choose the optimal method of treatment.The objective of the research was to optimize the diagnostic process when treating damaged coronal portion of the tooth after endodontic treatment through the determination of the percentage ratio of the volume of the coronal portion of the tooth to the total volume of tooth crown for different groups of teeth.Materials and methods. 42 extracted teeth with preserved coronal portions served as the material for our study. The volume of the coronal potion of the tooth as well as the total volume of tooth crown was determined using the method developed by us. Having calculated the ratio of the volume of the coronal portion of the tooth to the volume of tooth crown, we received the volume which is occupied by the coronal cavity of the tooth.Results. We obtained the percentage ratio of the volume which is occupied by the coronal cavity of the tooth compared to its crown for different groups of teeth.Conclusions. To estimate the extent of damage to the coronal portion of the tooth after endodontic treatment, it is recommend using the obtained data, namely, in the maxillary and mandibular front teeth, the coronal portion of the tooth occupies up to 10% of tooth crown volume, while in the maxillary and mandibular grinding teeth, the coronal portion of the tooth occupies up to 5% of tooth crown volume.


2020 ◽  
Vol 11 ◽  
Author(s):  
Torsten Sterzenbach ◽  
Anne Pioch ◽  
Martin Dannemann ◽  
Christian Hannig ◽  
Marie-Theres Weber

Bacterial infections of root canals and the surrounding dental hard tissue are still a challenge due to biofilm formation as well as the complex root canal anatomy. However, current methods for analyzing biofilm formation, bacterial colonization of root canals and dental hard tissue [e.g., scanning electron microscopy, confocal laser scanning microscopy (CLSM) or determination of colony forming units (CFU)] are time-consuming and only offer a selective qualitative or semi-quantitative analysis. The aim of the present study is the establishment of optimized molecular biological methods for DNA-isolation and quantification of bacterial colonization via quantitative PCR (qPCR) from dental hard tissue. Root canals of human premolars were colonized with Enterococcus faecalis. For isolation of DNA, teeth were then grinded with a cryo mill. Since the hard tissues dentin and especially enamel belong to the hardest materials in the human organism, the isolation of bacterial DNA from root dentin is very challenging. Therefore, treatment steps for the isolation of DNA from grinded teeth were systematically analyzed to allow improved recovery of bacterial DNA from dental hard tissues. Starting with the disintegration of the peptidoglycan-layer of bacterial cells, different lysozyme solutions were tested for efficacy. Furthermore, incubation times and concentrations of chelating agents such as EDTA were optimized. These solutions are crucial for the disintegration of teeth and hence improve the accessibility of bacterial DNA. The final step was the determination of prior bacterial colonization of each root canal as determined by qPCR and comparing the results to alternative methods such as CFU. As a result of this study, optimized procedures for bacterial DNA-isolation from teeth were established, which result in an increased recovery rate of bacterial DNA. This method allows a non-selective and straightforward procedure to quantify bacterial colonization from dental hard tissue. It can be easily adapted for other study types such as microbiome studies and for comparable tissues like bones.


2018 ◽  
Author(s):  
Yiming Zhao ◽  
Huy van Nguyen ◽  
Louise Male ◽  
Philip Craven ◽  
Benjamin R. Buckley ◽  
...  

<div>Twelve 1,5-disubtituted and fourteen 5-substituted 1,2,3-triazole derivatives bearing diaryl or dialkyl phosphines at the 5-position were synthesised and used as ligands for palladium-catalysed Suzuki-Miyaura cross-coupling reactions. Bulky substrates were tested, and lead-like product formation was demonstrated. The online tool SambVca 2.0 was used to assess steric parameters of ligands and preliminary buried volume determination using XRD obtained data in a small number of cases proved to be informative. Two modelling approaches were compared for the determination of</div><div>the buried volume of ligands where XRD data was not available. An approach with imposed steric restrictions was found to be superior in leading to buried volume determinations that closely correlate with observed reaction conversions. The online tool LLAMA was used to determine lead-likeness of potential Suzuki-Miyaura cross-coupling products, from which ten of the most lead-like were successfully synthesised. Thus, confirming these readily accessible triazole-containing phosphines as highly suitable ligands for reaction screening and optimisation in drug discovery campaigns.</div>


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