scholarly journals Development of a cell-free protein synthesis system for practical use

2021 ◽  
Vol 97 (5) ◽  
pp. 261-276
Author(s):  
Yaeta ENDO
Keyword(s):  
Protein Synthesis ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

Translational incorporation of multiple unnatural amino acids in a cell-free protein synthesis system

2014 ◽  
Vol 19 (3) ◽  
pp. 426-432 ◽  
Author(s):  
Su-Jin Oh ◽  
Kyung-Ho Lee ◽  
Ho-Cheol Kim ◽  
Christy Catherine ◽  
Hyungdon Yun ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

scholarly journals Cloning-Independent Expression and Analysis of ω-Transaminases by Use of a Cell-Free Protein Synthesis System

2010 ◽  
Vol 76 (18) ◽  
pp. 6295-6298 ◽  
Author(s):  
Yong-Chan Kwon ◽  
Kyung-Ho Lee ◽  
Ho-Cheol Kim ◽  
Kyuboem Han ◽  
Joo-Hyun Seo ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Function ◽  
Free Expression ◽  
Synthesis System ◽  
Free Protein ◽  
Enzyme Substrate ◽  
Information Gap ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

ABSTRACT Herewith we report the expression and screening of microbial enzymes without involving cloning procedures. Computationally predicted putative ω-transaminase (ω-TA) genes were PCR amplified from the bacterial colonies and expressed in a cell-free protein synthesis system for subsequent analysis of their enzymatic activity and substrate specificity. Through the cell-free expression analysis of the putative ω-TA genes, a number of enzyme-substrate pairs were identified in a matter of hours. We expect that the proposed strategy will provide a universal platform for bridging the information gap between nucleotide sequence and protein function to accelerate the discovery of novel enzymes.

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