scholarly journals Concordance between results of serum protein electrophoresis of pathological serum by cellulose acetate and capillary zone electrophoresis

2005 ◽  
Vol 49 (2) ◽  
pp. 45-51
Author(s):  
Hiroshi Ihara ◽  
Takayuki Matsumoto ◽  
Takashi Kakinoki ◽  
Yutaka Aoki ◽  
Naotaka Hashizume ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Capillary Zone Electrophoresis ◽  
Serum Protein ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Zone Electrophoresis ◽  
Capillary Zone

Case report: Interference from isatuximab on serum protein electrophoresis prevented demonstration of complete remission in a myeloma patient

2021 ◽  
pp. 000456322110620
Author(s):  
Rachel D Wheeler ◽  
Micsha V Costa ◽  
Asante Crichlow ◽  
Fenella Willis ◽  
Yasmin Reyal ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Complete Remission ◽  
Capillary Zone Electrophoresis ◽  
Serum Protein ◽  
Plasma Cells ◽  
Survival Rates ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Zone Electrophoresis ◽  
Capillary Zone

Multiple myeloma is a haematological cancer caused by malignant plasma cells in the bone marrow that can result in organ dysfunction and death. Recent novel treatments have contributed to improved survival rates, including monoclonal antibody therapies that target the CD38 protein on the surface of plasma cells. Anti-CD38 therapies are IgG kappa monoclonal antibodies that are given in doses high enough for the drug to be visible on serum protein electrophoresis as a small paraprotein. We present a case where isatuximab, the most recent anti-CD38 monoclonal antibody to be approved for treatment of myeloma, obscured the patient’s paraprotein on gel immunofixation, so that complete remission could not be demonstrated. This was resolved using the isatuximab Hydrashift assay. The interference on gel immunofixation was unexpected because isatuximab migrated in a position distinct from the patient’s paraprotein on capillary zone electrophoresis. We demonstrate the surprising finding that isatuximab migrates in a different position on gel electrophoresis compared to capillary zone electrophoresis. It is vital that laboratories are aware of the possible interference on electrophoresis from anti-CD38 monoclonal antibody therapies, and are able to recognise these drugs on protein electrophoresis. The difference in isatuximab’s electrophoretic mobility on capillary and gel protein electrophoresis makes this particularly challenging. Laboratories should have a strategy for alternative analyses in the event that the drugs interfere with assessment of the patient’s paraprotein.

scholarly journals Multicenter evaluation of the Paragon CZETM 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing

1998 ◽  
Vol 44 (3) ◽  
pp. 599-605 ◽  
Author(s):  
Jacques Bienvenu ◽  
Maria Stella Graziani ◽  
François Arpin ◽  
Hélène Bernon ◽  
Cynthia Blessum ◽  
...  
Keyword(s):  
Serum Protein ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Similar Data ◽  
Cellulose Acetate Electrophoresis ◽  
Zone Electrophoresis ◽  
Γ Globulins ◽  
Capillary Zone ◽  
Electrophoresis System ◽  
Monoclonal Component

Abstract Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZETM 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were <2% for albumin and γ-globulins and 4–7% for α1-, α2-, and β-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was <0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations >10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.

scholarly journals Prospective Study of Serum Protein Capillary Zone Electrophoresis and Immunotyping off Monoclonal Proteins by Immunosubtraction

1998 ◽  
Vol 110 (4) ◽  
pp. 503-509 ◽  
Author(s):  
Jerry A. Katzmann ◽  
Raynell Clark ◽  
Elizabeth Sanders ◽  
James P. Landers ◽  
Robert A. Kyle
Keyword(s):  
Prospective Study ◽  
Capillary Zone Electrophoresis ◽  
Serum Protein ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Monoclonal Proteins

scholarly journals Quantitative determination of HbA2 level in β-thalassemia trait by using Capillary Zone Electrophoresis in Balochistan; A comparative study

2020 ◽  
Vol 2 (3) ◽  
pp. 56-61
Author(s):  
Naseeb Ullah ◽  
Muhmmad Ali Khan ◽  
Shafi Muhammad Khosa ◽  
Mohsin Ali ◽  
Ashique Hussain ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Cellulose Acetate ◽  
Capillary Zone Electrophoresis ◽  
Iron Deficiency Anemia ◽  
Thalassemia Major ◽  
Vital Role ◽  
Deficiency Anemia ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Thalassemia Trait

In Pakistan, β-thalassemia is considered as one of most typical genetic abnormality. Different countries like Itlay, France, and Greece have recognised thalassemia as preventive disorder of society. In Pakistan, since 1994 different efforts were made to control thalassemic epidemic. Inspite of this, there is no significant decline in occurrence of childbirth with transfusion dependent β-thalassemia incidents. In order to obtain fruitful results of general thalassemia eradication program nation wide,  it is vital to evaluate β-thalassemia trait (BTT) by specific, low price, and accurate mechanism of diagnosis. So, this current research was carried out to decide the levels of HbA2 in samples by the use of Capillary Zone Electrophore and Cellulose Acetate Hemoglobin Electrophoresis. This comparative research was done in the four groups:  i) Normal Individual having no sign or symptom of thalassemia ii) Patients having BTT i.e b-thalassemia trait iii) Patients having IDA i.e Iron Deficiency Anemia   iv) Parents of β-thalassemia major child with iron deficiency anemia (BTT and IDA). It means that both parents are carrier of β-thalassemia not diagnosed yet. The level of HbA2 were estimated by the Cellulose Acetate Hemoglobin Electrophoresis and Capillary Zone Electrophoresis independently and then compared to design established results. Result: Both these methods have found to be correct for the patients having b-thalassemia trait (BTT) but in combined condition, cases of b-thalassemia trait (BTT) were failed to be analyzed by both these systems. Conclusion: As this technique is very easy, easily reachable and affordable. So, it is highly recommended for the diagnosis in the poor province like Balochistan in Pakistan. In thalassemia prevention, it plays a vital role.  

Underestimation of monoclonal proteins by serum protein electrophoresis on cellulose acetate.

1987 ◽  
Vol 33 (1) ◽  
pp. 182-184 ◽  
Author(s):  
F J Liu ◽  
H A Fritsche ◽  
J M Trujillo
Keyword(s):  
Cellulose Acetate ◽  
Serum Sample ◽  
Serum Protein ◽  
Protein Concentration ◽  
Protein Electrophoresis ◽  
Response To Therapy ◽  
Serum Protein Electrophoresis ◽  
M Protein ◽  
Albumin Concentration ◽  
Undiluted Serum

Abstract Our study of 95 serum samples from 37 patients with monoclonal gammopathy revealed distorted irregular monoclonal (M) protein bands after serum protein electrophoresis (SPE) on cellulose acetate membrane. In 71 (75%) of the 95 sera, the M-protein was underestimated and the albumin concentration overestimated. Dilution of the serum sample before SPE eliminated the abnormality of the M-protein bands. By SPE, the mean albumin concentration in these 71 undiluted sera was 45.8 (SD 7.4) g/L vs 37.9 (SD 5.8) g/L for the diluted sera; moreover, this was true of individual samples: measured albumin concentration in each diluted serum sample was always less than in the undiluted serum. As measured by the bromcresol green dye-binding method, the albumin concentration was 32.8 (SD 5.9) g/L. Similarly, the M-protein concentration in SPE was 49.5 (SD 12.3) g/L for the undiluted sera vs 61.8 (SD 15.1) g/L for the diluted sera, and the M-protein concentration in each diluted serum sample always exceeded that in the undiluted serum. Underestimation of M-protein limits the usefulness of M-protein measurement in evaluating the patient's response to therapy and for early detection of disease progression. SPE strips should be carefully inspected visually, and sera with M-protein band abnormalities should be diluted and re-assayed if SPE is to quantify concentrations of M-protein and albumin accurately.

Computer Calculation of Serum Protein Electrophoresis, Based on Peak Height Measurements

1970 ◽  
Vol 16 (9) ◽  
pp. 760-762 ◽  
Author(s):  
Sylvan M Sax ◽  
John J Moore
Keyword(s):  
Computer Program ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Computer Calculation ◽  
Peak Height ◽  
Protein Electrophoresis ◽  
Protein Fractions ◽  
Serum Protein Electrophoresis ◽  
Random Errors ◽  
Routine Application

Abstract A computer program has been written for off-line calculation of relative and absolute percentages of the five serum protein fractions usually seen on cellulose acetate electrophoretograms, with use of manually observed peak heights on densitometer scans. With myeloma-like peaks, both peak height and width at half-peak height must be measured. Routine application of the program saves technician time and decreases the number of large random errors.

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