Kinetic Modeling and Sensitivity Analysis of Kinetic Parameters for L-Glutamic Acid Production Using Corynebacterium glutamicum

2009 ◽  
Vol 7 (1) ◽  
Author(s):  
S. Suresh ◽  
Noor Salam Khan ◽  
Vimal Chandra Srivastava ◽  
Indra Mani Mishra
Keyword(s):  
Experimental Data ◽  
Sensitivity Analysis ◽  
Kinetic Model ◽  
Glutamic Acid ◽  
Corynebacterium Glutamicum ◽  
Negative Impact ◽  
Product Formation ◽  
Biochemical Engineering ◽  
Glutamic Acid Production ◽  
Engineering Journal

In the present work, a kinetic model has been developed for the batch fermentation of glucose for the production of L-glutamic acid (LGA) by Corynebacterium glutamicum. Parameters of the kinetic model have been determined by using the experimental data available in literature and the results have been compared by carrying out computer simulation. The kinetic model proposed in the present study provides better predictions for the growth of biomass, substrate consumption and LGA production as compared to the prediction reported by Bona and Moser (Bioprocess Engineering 17(2) (1997b) 121-125). The present model also fits the experimental data given by Zhang et al. (Enzyme and Microbial Technology, 22, 205-209, 1998) and Khan et al. (Biochemical Engineering Journal, 25, 173–178, 2005), respectively. Sensitivity analysis was carried out to assess the validity of the developed model, and to reveal the kinetic parameter that has maximum impact on LGA production. It is found that an increase in the non-growth associated product formation coefficient caused highest negative impact on LGA production.

Effect of different carbon sources on aerobic storage by activated sludge

2002 ◽  
Vol 45 (6) ◽  
pp. 157-168 ◽  
Author(s):  
M. Beccari ◽  
D. Dionisi ◽  
A. Giuliani ◽  
M. Majone ◽  
R. Ramadori
Keyword(s):  
Experimental Data ◽  
Activated Sludge ◽  
Glutamic Acid ◽  
Carbon Sources ◽  
Product Formation ◽  
Substrate Removal ◽  
Product Profile ◽  
Observed Behaviour ◽  
Real Wastewater ◽  
And Storage

A study of substrate removal by real activated sludge with several synthetic substrates (acetate, ethanol, glutamic acid) and wastewater (raw and filtered) was carried out. Substrate, stored compounds (polyhydroxyalkanoates, PHA and internal carbohydrates), ammonia and oxygen uptake rate (OUR) were analytically determined. Polyhydroxybutyrate (PHB) was stored when the substrate was acetate or ethanol, while no appreciable formation of storage compound was detected using glutamic acid. A low amount of PHB was also formed in tests with raw and filtered wastewater which was probably mainly due to its acetate content. As far as the sum of storage and growth (indirectly estimated through ammonia consumption) did not match the overall solids formation, other unidentified mechanisms of substrate removal were likely to occur (biosorption, accumulation and/or storage of unidentified compounds). ASM3 and two derived models were used in the interpretation of experimental data with reference to synthetic substrates. With reference to synthetic substrates ASM3 can well describe the experimental data only assuming a stored product formation much higher than the analytically detected one, whereas the model that assumes a parallel growth and storage on the substrate can well describe the observed stored product profile only assuming a direct contribution of growth much higher than estimated from ammonia consumption. The model that assumes an accumulation/biosorption stage as first step of substrate removal can better describe the whole experimentally observed behaviour. However as well as in ASM3 this implies that some fraction of removed COD is still unidentified. With reference to real wastewater where the different phenomena were mixed up due to the presence of several substrates, the different models gave similar results.

Further Studies of the Kinetics of Oxygenation of Arachidonic Acid by Soybean Lipoxygenase

1975 ◽  
Vol 53 (11) ◽  
pp. 1220-1231 ◽  
Author(s):  
H. W. Cook ◽  
W. E. M. Lands
Keyword(s):  
Experimental Data ◽  
Arachidonic Acid ◽  
Kinetic Model ◽  
Theoretical Data ◽  
Product Formation ◽  
Soybean Lipoxygenase ◽  
Product Removal ◽  
Velocity Changes ◽  
Extensive Experimental Data ◽  
Kinetics Of

A kinetic model for soybean lipoxygenase (EC 1.13.11.12) has been examined by comparing results from extensive experimental data with theoretical data generated from a computer program. Kinetic constants have been established by closely fitting experimental and computer-generated data with both product formation versus time, and the more complex accelerative and decelerative relationships of velocity changes with time.It has been confirmed that activation of lipoxygenase by its hydroperoxide product is necessary for activity, and product removal gives inhibition in a manner quantitatively predicted by the model. The earliest accurate measurement of velocity (at 9 s) is a convenient index of the amount of product–activator present in reaction mixtures, and can be used to assay quantitatively the amount of product–activator.The results confirm that soybean lipoxygenase catalyzes a product-activated, substrate-inhibited oxygenation accompanied by a self-catalyzed destruction of its activity.

scholarly journals High production of L-glutamic acid from date juice extracts by Corynebacterium glutamicum using fed-batch cultures: pulsed and continuous feeding modes

2021 ◽  
Vol 8 (1) ◽  
pp. 37-50
Author(s):  
Mouffok Abdenacer ◽  
Nancib Aicha ◽  
Boudrant Joseph ◽  
Nancib Nabil
Keyword(s):  
Glutamic Acid ◽  
Corynebacterium Glutamicum ◽  
Ammonium Sulfate ◽  
Batch Culture ◽  
Acid Production ◽  
Fed Batch ◽  
Feeding Modes ◽  
Continuous Feeding ◽  
Date Juice ◽  
Glutamic Acid Production

Abstract In the present work, L-glutamic acid production by Corynebacterium glutamicum fermentation on date juice extracts applying two fed-batch feeding modes, pulsed and continuous, were investigated. According to the obtained results, the continuous feeding fed-batch mode was found to be the most efficient process. Moreover the continuous feeding rate mode with a feeding medium containing date juice sugars enriched with ammonium sulfate was found even more favorable as it enhances the L-glutamic acid production by approximately 2.35 fold more than the batch culture and by about 1.17 fold more than the pulsed feeding. In this respect, comparing the traditional batch culture to the continuously fed culture with a medium containing date juice sugars with ammonium sulfate showed increases of 135.47% in L-glutamic acid production, 104% in productivity, 39.09% in biomass, and 47.69% in the yield respectively allowing us to reach a final L-glutamic acid concentration of about 138 g/L, the highest ever published.

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