scholarly journals Performance of CO1 and ITS2 nested PCR in molecular identification of ordinary scabies (Sarcoptes scabiei var. hominis)

2021 ◽  
Vol 26 (4) ◽  
pp. 183
Author(s):  
Gita Dwi Prasasty ◽  
Miftahurrizqiyah Miftahurrizqiyah ◽  
Chairil Anwar ◽  
Dwi Handayani ◽  
Dalilah Dalilah ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Microscopic Examination ◽  
Nested Pcr ◽  
Gold Standard ◽  
Standard Technique ◽  
Sarcoptes Scabiei ◽  
High Prevalence ◽  
Diagnostic Test Result ◽  
Test Result ◽  
Gold Standard Technique

Scabies is a global disease with a high prevalence, causing morbidity and even mortality, especially in poor and developing countries. However, it is often misdiagnosed due to varied and unspecified lesions. The gold standard technique for diagnosis is a microscopic examination, which requires experienced experts in finding mites, mainly in ordinary scabies. CO1 and ITS2 genes have been widely used in molecular identification to detect Sarcoptes scabiei and its variants. This study aimed to determine and compare the sensitivity and specificity of CO1 and ITS2 S. scabiei genes to the microscopic examination of scabies skin scrapings. The skin scrapings of 52 subjects with scabies diagnosed by anamnesis, physical examination, and dermoscopic examination were examined under a microscope and analyzed by nested PCR. The diagnostic test result showed that the sensitivity of nested PCR of both CO1 and ITS2 genes to micro‐ scope examination was 100%. However, the specificity of both CO1 and ITS2 nested PCR was poor (24% and 0%). Hence, CO1 and ITS2 nested PCR could be more suitable for screening ordinary scabies in humans than the microscopic examination.

scholarly journals Isolated Arthroscopic Radial Styloidectomy: The Three-Portal Approach

2020 ◽  
Vol 09 (04) ◽  
pp. 353-356
Author(s):  
Guillaume Herzberg ◽  
Marion Burnier
Keyword(s):  
Gold Standard ◽  
Standard Technique ◽  
New Technique ◽  
Clear Definition ◽  
Current Gold Standard ◽  
Clear View ◽  
A New Technique ◽  
Portal Technique ◽  
Gold Standard Technique ◽  
Technical Trick

Abstract Background The current gold-standard technique for radial styloidectomy is arthroscopic. The use of only two dorsal portals may not allow a crystal-clear view of the dorsal arthritic rim of the radius because of the dorsal capsule synovitis. Objectives To propose a new technique for isolated arthroscopic radial styloidectomy. The addition of a volar radial portal and a sequential procedure are presented. Patients and Methods Two trochars for alternatively viewing from volar radial or 3–4 are used. A 1–2 portal is used for instrumentation with a motorized burr. We have been using the three-portal technique in 34 cases (26 isolated). Results This modification of the classic arthroscopic radial styloidectomy is technically easy and the view of the dorsal rim of the radius provides a clear definition and treatment of the dorso-radial arthritic pathology. Conclusions The authors recommend this technical trick to perform an easy and comprehensive isolated palliative or curative arthroscopic radial styloidectomy.

scholarly journals Clinical Case Employing Two Different Biomaterials in Bone Regeneration

2020 ◽  
Vol 10 (13) ◽  
pp. 4516
Author(s):  
Roberto Ghiretti ◽  
Carlo F. Grottoli ◽  
Alberto Cingolani ◽  
Giuseppe Perale
Keyword(s):  
Bone Regeneration ◽  
Gold Standard ◽  
Bone Substitute ◽  
Clinical Case ◽  
Hybrid Approach ◽  
Standard Technique ◽  
Surgical Strategies ◽  
Calcium Phosphosilicate ◽  
Gold Standard Technique

The case of a 59-year-old woman lacking bone in the lower left side of her mandible, and treated with two different biomaterials for bone regeneration, is reported here. Specifically, two different anatomical sites damaged by two different pathologies were studied: a radicular fracture and peri-implantitis. The sites were treated via xenograft bone substitute and calcium phosphosilicate, respectively. Follow-up evaluations showed that the two different methodologies employing different materials in the same organism undergoing the same metabolic processes achieved the same good results. This represents a significant change in current surgical strategies for the dental region: instead of focusing on a single gold-standard technique, it is possible to follow a hybrid approach by adapting the biomaterial and the protocol used to the specificities of the defect.

scholarly journals Open Partial Nephrectomy in Renal Cancer: A Feasible Gold Standard Technique in All Hospitals

2008 ◽  
Vol 2008 ◽  
pp. 1-9 ◽  
Author(s):  
J. M. Cozar ◽  
M. Tallada
Keyword(s):  
Partial Nephrectomy ◽  
Gold Standard ◽  
Radical Nephrectomy ◽  
Young Patients ◽  
Standard Technique ◽  
Tumor Diameter ◽  
Cancer Specific Survival ◽  
Renal Masses ◽  
Open Partial Nephrectomy ◽  
Gold Standard Technique

Introduction. Partial nephrectomy (PN) is playing an increasingly important role in localized renal cell carcinoma (RCC) as a true alternative to radical nephrectomy. With the greater experience and expertise of surgical teams, it has become an alternative to radical nephrectomy in young patients when the tumor diameter is 4 cm or less in almost all hospitals since cancer-specific survival outcomes are similar to those obtained with radical nephrectomy.Materials and Methods. The authors comment on their own experience and review the literature, reporting current indications and outcomes including complications. The surgical technique of open partial nephrectomy is outlined.Conclusions. Nowadays, open PN is the gold standard technique to treat small renal masses, and all nonablative techniques must pass the test of time to be compared to PN. It is not ethical for patients to undergo radical surgery just because the urologists involved do not have adequate experience with PN. Patients should be involved in the final treatment decision and, when appropriate, referred to specialized centers with experience in open or laparoscopic partial nephrectomies.

scholarly journals A rare case of acute intestinal obstruction due to bowel entrapment in migrated onlay polypropylene mesh

2014 ◽  
Vol 96 (7) ◽  
pp. e4-e5 ◽  
Author(s):  
SK Tiwari ◽  
Pawanindra Lal
Keyword(s):  
Intestinal Obstruction ◽  
Gold Standard ◽  
Rare Case ◽  
Standard Technique ◽  
Acute Intestinal Obstruction ◽  
Mesh Migration ◽  
Intraperitoneal Mesh ◽  
Onlay Mesh ◽  
Mesh Hernioplasty ◽  
Gold Standard Technique

Onlay mesh hernioplasty is the gold standard technique in the management of inguinal hernias. We report a rare case of intraperitoneal mesh migration with bowel entrapment causing acute intestinal obstruction in an elderly patient.

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