scholarly journals Characterization of Streptomyces spp. Producing Indole-3-acetic acid as Biostimulant Agent

2015 ◽  
Vol 18 (2) ◽  
pp. 83 ◽  
Author(s):  
Charlie Ester De Fretes ◽  
Langkah Sembiring ◽  
Yekti Asih Purwestri
Keyword(s):  
Acetic Acid ◽  
Bacterial Culture ◽  
Cyperus Rotundus ◽  
Malt Extract ◽  
Streptomyces Sp ◽  
Iaa Production ◽  
Streptomyces Spp ◽  
Layer Chromatography ◽  
Indole 3 Acetic Acid

Twenty six isolates of Streptomyces spp. obtained from Cyperus rotundus L. rhizosphere were tested forability to produce indole-3-acetic acid (IAA) in yeast malt extract (YM) medium containing 2 mg/mL tryptophan.Screening of the isolates for ability to produce IAA was carried out by adding Salkowski reagent in bacteriaculture and was measured quantitatively by spectrophotometer at λ 530 nm. Thin Layer Chromatography (TLC)method was used to determine IAA. To ensure the IAA production in Streptomyces isolates, gene involved inIAA biosynthesis was detected by amplifying Tryptophan Monooxigenase (iaaM) gene. The study of the effectof tryptophan on the production of IAA was measured at different concentrations of tryptophan (0, 1, 2, 3,4, 5 mg/mL) in the bacterial culture. The result showed that there were two Streptomyces spp. isolates whichcould produce IAA, namely the isolates of Streptomyces sp. MS1 (125.48 μg/mL) and Streptomyces sp. BR27(104.13 μg/mL). The TLC result showed that the compound in both isolates was identifi ed to be IAA. Theamplifi cation results showed that iaaM gene was detected in both isolates. This results indicated that the IAMpathway is predicted involved in the biosynthesis of IAA in the selected isolates. Both of the isolates were ableto produce IAA after 24 h incubation and the highest production was at 120 h incubation with the concentrationof tryptophan was 2 mg/mL dan 1 mg/mL, respectively. Therefore, it is concluded that Streptomyces spp.isolates are able to produce IAA and potentially to be utilized as biostimulat agent. Keywords: Streptomyces spp., indole-3-acetic acid (IAA), indole-3-acetamide (IAM), Tryptophan Monooxigenasegene (iaaM)

scholarly journals Production of Indole-3-Acetic Acid: A White Biotechnology for Weed Biocontrol

2021 ◽  
Author(s):  
Sakaoduoen Bunsangiam ◽  
Nutnaree Thongpae ◽  
Savitree Limtong ◽  
Nantana Sri
Keyword(s):  
Acetic Acid ◽  
Cost Effective ◽  
Pilot Scale ◽  
Cyperus Rotundus ◽  
Shake Flask Culture ◽  
Iaa Production ◽  
Weed Growth ◽  
Fold Reduction ◽  
The One ◽  
Indole 3 Acetic Acid

Abstract Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade L-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3,569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.

scholarly journals Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sakaoduoen Bunsangiam ◽  
Nutnaree Thongpae ◽  
Savitree Limtong ◽  
Nantana Srisuk
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
Pilot Scale ◽  
Inhibitory Effect ◽  
Cyperus Rotundus ◽  
Shake Flask Culture ◽  
Scale Production ◽  
Iaa Production ◽  
Weed Growth ◽  
Indole 3 Acetic Acid

AbstractIndole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade l-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.

scholarly journals Identification endophytic fungi of Nutgrass (Cyperus rotundus L.) as solubilizing phosphate and indole-3-acetic acid producers

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Nur Kusmiyati ◽  
Septian Tri Wicaksono ◽  
Durrotul Maknuna
Keyword(s):  
Acetic Acid ◽  
Molecular Analysis ◽  
Endophytic Fungi ◽  
Cyperus Rotundus ◽  
Its Sequences ◽  
Isolation And Purification ◽  
Iaa Production ◽  
Rdna Its ◽  
Rdna Its Sequences ◽  
Indole 3 Acetic Acid

Low phosphate content in the soil can cause insufficient plant needs. Besides, the endophytic fungi of nutgrass have the potential as a phosphate solvent and can produce IAA (Indole-3-Acetic Acid). This study aimed to determine the levels of solubilizing phosphate and production of IAA (Indole-3-Acetic Acid) by the endophytic fungi of nutgrass and to identify the isolates based on rDNA-ITS sequences. The methods used were the isolation of endophytic fungi, analysis of solubilizing levels of phosphate and IAA production, and molecular analysis with rDNA-ITS sequences. Results of isolation and purification, found five isolates coded URT1, URT2, URT3, URT4, and URT5. The endophytic fungi of nutgrass were able to solubilizing phosphate levels around 54.03 - 87.83 ppm, with the highest levels produced by URT4 isolate. IAA levels around 5.58 - 45.50 ppm, with the highest levels produced by URT1 isolate. Based on molecular analysis with rDNA-ITS sequences, it showed that URT4 had 97.42% similarity to Aspergillus tereus species, while UTR1 had 100% similarity to Fusarium oxyporum species. To conclude, the endophytic fungi of nutgrass from A. tereus and F. oxyporum species have high levels of solubilizing phosphate and IAA production so that they are potential candidates for biofertilizer.

scholarly journals Furanone Derivatives from Terrestrial Streptomyces spp

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700
Author(s):  
Muhammad Arfan ◽  
Khaled A. Shaaban ◽  
Anja Schüffler ◽  
Hartmut Laatsch
Keyword(s):  
Acetic Acid ◽  
Quorum Sensing ◽  
Ferulic Acid ◽  
Structure Elucidation ◽  
Mass Spectra ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
First Time ◽  
Indole 3 Acetic Acid ◽  
New Metabolites

Chemical investigation of the terrestrial Streptomyces sp. isolates GT2005/020 and ANK148 led to the isolation of two microbial furanone derivatives, 5-hydroxy-4-methylnaphtho[1,2-b]furan-3-one (1) and 4-hydroxy-5-methyl-furan-3-one (2), respectively, which have some similarity to quorum sensing molecules of the AI-2 type. In addition, the known compounds chalcomycin, ferulic acid, indole-3-acetic acid, uracil, thymine, 2′-deoxy-thymidin, monensin B (3), phencomycin, and 1-acetyl-β-carboline were isolated. The structures of 1 and 2 were deduced from extensive studies of NMR (1D and 2D) and mass spectra. Additionally, the complete NMR shift assignments for monensin B (3) using H-H COSY, HMQC and HMBC experiments are reported here for the first time. We are describing the taxonomy and fermentation of the producing strains, the structure elucidation of the new metabolites and their bioactivity.

scholarly journals Volatile 1-octanol of tea (Camellia sinensis L.) fuels cell division and indole-3-acetic acid production in phylloplane isolate Pseudomonas sp. NEEL19

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Poovarasan Neelakandan ◽  
Chiu-Chung Young ◽  
Asif Hameed ◽  
Yu-Ning Wang ◽  
Kui-Nuo Chen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cell Division ◽  
Petri Dish ◽  
Gas Chromatography Mass Spectrometry ◽  
Dependent Manner ◽  
Tea Leaves ◽  
Acetic Acid Production ◽  
Iaa Production ◽  
Solvent Tolerant ◽  
Indole 3 Acetic Acid

AbstractTea leaves possess numerous volatile organic compounds (VOC) that contribute to tea’s characteristic aroma. Some components of tea VOC were known to exhibit antimicrobial activity; however, their impact on bacteria remains elusive. Here, we showed that the VOC of fresh aqueous tea leaf extract, recovered through hydrodistillation, promoted cell division and tryptophan-dependent indole-3-acetic acid (IAA) production in Pseudomonas sp. NEEL19, a solvent-tolerant isolate of the tea phylloplane. 1-octanol was identified as one of the responsible volatiles stimulating cell division, metabolic change, swimming motility, putative pili/nanowire formation and IAA production, through gas chromatography-mass spectrometry, microscopy and partition petri dish culture analyses. The bacterial metabolic responses including IAA production increased under 1-octanol vapor in a dose-dependent manner, whereas direct-contact in liquid culture failed to elicit such response. Thus, volatile 1-octanol emitting from tea leaves is a potential modulator of cell division, colonization and phytohormone production in NEEL19, possibly influencing the tea aroma.

scholarly journals A Quantitative Method for Determining Soil Populations of Streptomyces and Differentiating Potential Potato Scab-Inducing Strains

Plant Disease ◽  
1998 ◽  
Vol 82 (6) ◽  
pp. 631-638 ◽  
Author(s):  
Kenneth L. Conn ◽  
Edlira Leci ◽  
Giora Kritzman ◽  
George Lazarovits
Keyword(s):  
Potato Scab ◽  
Colony Morphology ◽  
Malt Extract ◽  
Facilitated Diffusion ◽  
Thaxtomin A ◽  
Streptomyces Spp ◽  
Plastic Bags ◽  
Large Numbers ◽  
History Of ◽  
Layer Chromatography

A procedure is described for estimating Streptomyces populations in soil. Soils are air-dried, 10g quantities are shaken in plastic bags containing 0.1% water agar and homogenized with a Stomacher homogenizer, serial dilutions are plated on a semi-selective culture (STR) medium and incubated for 2 weeks at 22°C, and the Streptomyces colonies are enumerated. Use of STR medium reduced the bacterial and fungal colonies recovered from soil to levels below that of the Streptomyces spp. while not affecting the number of Streptomyces colonies compared with those enumerated on yeast malt extract medium. A procedure for screening large numbers of Streptomyces strains for thaxtomin production, a phytotoxin recognized as a virulence marker in S. scabies, is also described. Strains are grown on oatmeal medium, and the thaxtomin is extracted from the medium by facilitated diffusion and detected by miniature thin layer chromatography. S. scabies and S. acidiscabies strains (approximately 130 from Ontario and 70 from other locations in North America) that produced thaxtomin did not form aerial mycelia or sporulate on STR medium within 2 weeks at 22°C. Ontario S. scabies strains that produced thaxtomin A also produced melanin on STR medium. All S. scabies strains from scab lesions that produced thaxtomin A had this colony morphology, whereas only 4 to 9% of strains from soil with this colony morphology produced thaxtomin A. Using these procedures, we determined that the population of thaxtomin-producing S. scabies in soil from a potato field in Ontario with a history of potato scab was about 20,000 CFU/g soil.

Characterization of the indole-3-acetic acid (IAA) biosynthetic pathway in an epiphytic strain of Erwinia herbicola and IAA production in vitro

1996 ◽  
Vol 42 (6) ◽  
pp. 586-592 ◽  
Author(s):  
M. Brandi ◽  
E. M. Clark ◽  
S. E. Lindow
Keyword(s):  
Acetic Acid ◽  
Pseudomonas Syringae ◽  
Pyruvic Acid ◽  
Enzyme Assays ◽  
Erwinia Herbicola ◽  
Iaa Production ◽  
Acid Pathway ◽  
Culture Supernatants ◽  
Indole 3 Acetic Acid

An epiphytic strain of Erwinia herbicola (strain 299R) synthesized indole-3-acetic acid (IAA) from indole-3-pyruvic acid and indole-3-acetaldehyde, but not from indole-3-acetamide and other intermediates of various IAA biosynthetic pathways in enzyme assays. TLC, HPLC, and GC–MS analyses revealed the presence of indole-3-pyruvic acid, indole-3-ethanol, and IAA in culture supernatants of strain 299R. Indole-3-acetaldehyde was detected in enzyme assays. Furthermore, strain 299R genomic DNA shared no homology with the iaaM and iaaH genes from Pseudomonas syringae pv. savastanoi, even in Southern hybridizations performed under low-stringency conditions. These observations strongly suggest that unlike gall-forming bacteria which can synthesize IAA by indole-3-acetamide, the indole-3-pyruvic acid pathway is the primary route for IAA biosynthesis in this plant-associated strain. IAA synthesis in tryptophan-supplemented cultures of strain 299R was over 10-fold higher under nitrogen-limiting conditions, indicating a possible role for IAA production by bacterial epiphytes in the acquisition of nutrients during growth in their natural habitat.Key words: indole-3-acetic acid, Erwinia, tryptophan, indole-3-pyruvic acid, nitrogen.

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