scholarly journals CYTOTOXIC ACTIVITY OF ALKALOIDS EXTRACTED FROM THREE IRAQI PLANTS AGAINST BREAST CANCER CELL LINE

2017 ◽  
Vol 10 (9) ◽  
pp. 78 ◽  
Author(s):  
Farah A Al-marzook ◽  
Rabab Omran
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Total Alkaloid ◽  
Cancer Cell Line ◽  
Total Alkaloids ◽  
Mcf 7

  Objectives: Screening for cytotoxic activity of total alkaloid extracts of Eucalyptus camaldulensis, Aloe vera, and Capparis spinosa against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and nontumorigenic fetal hepatic cell line (WRL-68).Methods: The plant powders were extracted separately with 80% methanol and chloroform at pH 2 and 10. Total alkaloids were detected qualitatively by Mayer’s, Dragendorff’s, and Hager‘s reagents and estimated quantitatively by bromocresol green spectrophotometry depending on the atropine calibration curve. The cytotoxic activity was evaluated by 3-[4, 5-dimethylthiazoyl]-2, 5-diphenyltetrazolium bromide assay.Results: The extract of E. camaldulensis had highest total alkaloid content (24.50±1.70 mg/100 g plant dry weight) than the others. The total alkaloids (400 μg/ml) of E. camaldulensis reduced the cell viability of both cell lines MCF-7 and WRL-68 to 45.25±2.20% and 92.00±1.55%, respectively, and the inhibitory concentration 50% of cells were 375.50 μg/ml for MCF-7. The alkaloids of C. spinosa had effect 79.80±7.08% and 89.50±0.09% against MCF-7and WRL-68, respectively. While the total alkaloids of A. vera had slightly effect on both cell lines.Conclusion: Plant alkaloids appeared variable cytotoxic activity against cancer and normal cell lines depending on the alkaloid contents, concentrations, purity, and cell line types.

scholarly journals CYTOTOXIC ACTIVITY OF ALKALOID EXTRACTS OF DIFFERENT PLANTS AGAINST BREAST CANCER CELL LINE

2017 ◽  
Vol 10 (7) ◽  
pp. 168
Author(s):  
Farah A Al-marzook ◽  
Rabab Omran
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Breast Cancer Cell Line ◽  
Total Alkaloid ◽  
Cancer Cell Line ◽  
Total Alkaloids ◽  
Alkaloid Extracts ◽  
Mcf 7

Objectives: To study in vitro cytotoxic activity of total alkaloid extracts of Pinus sabiniana L., Phoenix dactylifera L. and Ferocactus sp. L. against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and non-tumorigenic fetal hepatic cell line (WRL-68). Methods: Plant powder of each P. sabiniana L. leaves, P. dactylifera L. pollen grains, and Ferocactus sp. L. The leaves were extracted separately with 80% methanol, chloroform at pH 2 and pH 10 and the chloroform portion was dried to obtain the total alkaloid extracts. The total alkaloids were detected qualitatively by Mayer’s, Dragendorff’s and Hager’s reagents and estimated quantitatively by bromocresol green spectrophotometry depending on the atropine calibration curve. The cytotoxic activity was evaluated by 3-[4, 5-dimethylthiazoyl]-2, 5-diphenyltetrazolium bromide assay. Results: The extract of P. sabiniana L. had highest total alkaloid content (164.62±2.8 mg/100 g dry weight of plant) than the other plants (P. dactylifera l., Ferocactus sp. L.), the total alkaloids of Ferocactus sp. L. and P. dactylifera L., reduced the cell viability of both cell lines, the highest reduction occurred in the concentration 400 μg/ml was 46±2.20% (MCF-7) and 56.2±2.2% (WRL-68) for Ferocactus sp. L., followed by 56.2±2.2% (MCF-7) and 57.5±3.2% (WRL-68) for P. dactylifera L. The alkaloids of P. sabiniana was very lower effects on both cell lines MCF-7, and WRL-68 was 89.3±3.44% and 90.16±2.7%, respectively, at the same concentration. Conclusion: Plant alkaloids had variable effects against cancer and normal cell lines depending on the type of alkaloid compounds and their concentration in the extract.

scholarly journals Anticancer activity of chicken cathelicidin peptides against different types of cancer

2021 ◽  
Author(s):  
Maged Mostafa Mahmoud ◽  
Ahmed M. Al-Hejin ◽  
Turki S. Abujamel ◽  
Modhi Alenezi ◽  
Fadwa Aljoud ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
High Dose ◽  
Mcf 7

Abstract This study served as the pioneer in studying the anti-cancer role of chicken cathelicidin peptides. Chicken cathelicidins were used as anticancer agent against the breast cancer cell line (MCF-7) and human colon cancer cell line (HCT116). An in vivo investigation was also achieved to evaluate the role of chicken cathelicidin in Ehrlich ascites cell (EAC) suppression as a tumor model after subcutaneous implantation in mice. In addition, the mechanism of action of the interaction of cationic peptides with breast cancer cell line MCF-7 was also investigated. It was found during the study that exposure of cell lines to higher concentration of chicken cathelicidin for 72 h reduced cell lines growth rate by 90%-95%. These peptides demonstrated down-regulation of (cyclin A1 and cyclin D genes) which are essential for G1/S phase transient and S/G2 phase and consequently causes “prometaphase arrest” ultimately leading to death of MCF-7 cells. The study showed two- and three-times higher expression of the caspase-3, and − 7 genes respectively in MCF-7 cells treated with chicken peptides (especially cathelicidin-2 and − 3) relative to untreated cells which encouraged pro-apoptotic pathway, autophagy, and augmentation of the anti-proliferative activity. Our data showed that chicken ( CATH-1 ) enhance releasing of TNFα, INF-γ and upregulation of granzyme K in treated mice groups, in parallel, the tumor size and volume was reduced in the treated EAC-bearing groups after cathelicidin administration compared to untreated EAC-bearing group. Additionally, animals received high dose of cathelicidin-1 (40 µg/ml) displayed an apical survival rate compared to untreated carcinoma control and animals which received low dose of cathelicidin (10 and 20 µg/ml). Tumor of mice groups treated with chicken cathelicidin displayed high area of necrosis compared to untreated EAC-bearing mice. Based on histological analysis and immunohistochemical staining revealed that the tumor section in Ehrlich solid tumor exhibited a strong Bcl2 expression in untreated control compared to mice treated with 10 & 20 µg/ml of cathelicidin. Interestingly, low expression of Bcl2 were observed in mice taken 40 µg/ml of CATH-1. This study drive intention in treatment of cancer through the efficacy of anticancer efficacy of chicken cathelicidin peptides.

scholarly journals EXTRACTION OF PHENOLIC COMPOUNDS AS ANTIOXIDANTS FROM SOME PLANTS AND THEIR CYTOTOXIC ACTIVITY AGAINST BREAST CANCER CELL LINE

2017 ◽  
Vol 10 (7) ◽  
pp. 349
Author(s):  
Rabab Omran ◽  
Zahraa Mal Taee ◽  
Mohammed Jal Jassani ◽  
Hayder O Hashim
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Normal Cell ◽  
Cancer Cell Line ◽  
Mcf 7

Objective: The objective of the study was to study the antioxidants and cytotoxic activities of phenolic extracts of some plants against breast cancer and normal cell lines.Methods: Phenolics were extracted from different parts of some plants (15) such as seeds, fruits, leaves, and rhizomes using methanol: ethanol: HCl: Distilled water at a ratio 50:29:1:20 (v:v:v:v). The contents of phenolics and flavonoids were estimated using gallic acid and quercetin as standards, respectively. The antioxidant activity was determined by 2,2ˋ-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. Phenolic flavonoids were partially purified by adsorption chromatography using a silica gel column from selected plants and assayed their cytotoxic activity against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and a normal cell line of non-tumorigenic fetal hepatic cell line (WRL-68).Results: The Cinnamomum zeylanicum extract had the highest phenolic and flavonoid contents were followed by Lawsonia inermis, Citrullus colocynthis, Syzygium aromaticum, Peganum nigrum, and Phoenix dactylifera. The antioxidant activity of Curcuma longa, P. dactylifera, C. colocynthis, Solanum melongena, and C. zeylanicum extracts had the highest ability to scavenge the free radicals. The acetone fraction of P. dactylifera and C. colocynthis extracts had the minimum inhibition dose that kills 50% of cells inhibitory concentration 50 values 156.91 μg and 1055.06 μg against MCF-7 and 372.86 μg and 153.8 μg against WRL-68, respectively. While the S. melongena extract had less effect on both cell lines.Conclusions: Phenolics as antioxidant substances had moderate or variable effectiveness on normal and cancer cell lines, and the highest concentrations were cancerous poison impact may be on normal cells over than cancer cells.

scholarly journals Cytotoxic activity of Macrosolen parasiticus (L.) Danser on the growth of breast cancer cell line (MCF-7)

2015 ◽  
Vol 11 (42) ◽  
pp. 156 ◽  
Author(s):  
Richard Lobo ◽  
Nimmy Kumar ◽  
Rajalekshmi Maheshwari ◽  
CS Shreedhara ◽  
VijayKumar Sodde
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Mcf 7
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