scholarly journals CELLULAR UPTAKE STUDY AND CYTOTOXICITY STUDY OF RESVERATROL-GOLD-PEG-FOLATE (RSV-AU-PEG-FA) NANOPARTICLES ON HELA HUMAN CERVICAL CANCER CELL LINE

2020 ◽  
pp. 113-118
Author(s):  
DARA A. PUTRI ◽  
SUTRIYO SUTRIYO ◽  
FADLINA C. SAPUTRI
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cellular Uptake ◽  
Hela Cells ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Uptake Study ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Objective: This study aimed to evaluate the effectivity of resveratrol-gold-PEG-folate (RSV-Au-PEG-FA) nanoparticles formulation in resveratrol (RSV) targeted delivery and cytotoxicity effect on HeLa human cervical cancer cell line. Methods: Gold nanoparticles (AuNP) were used as carriers and folic acid (FA) was used as active targeting moiety, using polyethylene glycol-bis-amine (PEG-bis-amine) as linker. RSV-Au-PEG-FA nanoparticles were characterized by UV-Vis spectrophotometry, infrared spectroscopy, particle size analyzer (PSA), and transmission electron microscopy (TEM). Cellular uptake study was conducted by using fluorescence microscope. Cytotoxicity study was conducted by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: Cellular uptake study has shown that RSV-Au-PEG-FA nanoparticles are potential to be accumulated intracellularly in HeLa cells more than in Vero cells. Cytotoxicity study has shown RSV-Au-PEG-FA nanoparticles IC50 67.06±2.14 mM and RSV IC50 9.66±1.44 mM on HeLa cells Conclusion: RSV-Au-PEG-FA nanoparticles are potential to enhance RSV uptake by HeLa cells selectively.

Benzylisoquinoline alkaloids from Nelumbo nucifera Gaertn. petals with antiausterity activities against the HeLa human cervical cancer cell line

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Juthamart Maneenet ◽  
Ashraf M. Omar ◽  
Sijia Sun ◽  
Min Jo Kim ◽  
Supawadee Daodee ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Nelumbo Nucifera ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line ◽  
Benzylisoquinoline Alkaloids ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Abstract Ethanolic extract of Nelumbo nucifera petals showed preferential cytotoxic activity against HeLa human cervical cancer cell line with a PC50 value of 10.4 μg/mL. This active extract was subjected to a phytochemical investigation study which led to the isolation of nine benzylisoquinoline alkaloids (1–9). The isolated compounds exhibited potent antiausterity activities. Moreover, under nutrient-deprived conditions, (−)-lirinidine (8) induced remarkable alterations in HeLa cell morphology including cell shrinkage and plasma blebbing leading to total cell death within 10 h. Mechanistically, 8 was found to inhibit Akt/mTOR signaling pathway. It also induced apoptosis by promoting caspase-3 activation and inhibiting Bcl-2 expression. Therefore, benzylisoquinoline alkaloids skeleton can be considered as a promising scaffold for the anticancer drug development against cervical cancer.

scholarly journals The restraining effect of baicalein and U0126 on human cervical cancer cell line HeLa

2017 ◽  
Vol 16 (1) ◽  
pp. 957-963 ◽  
Author(s):  
Haiqiong Ye ◽  
Yujiao Zhang ◽  
Yongzhou Wang ◽  
Jiyi Xia ◽  
Xiguang Mao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line ◽  
Cell Line Hela ◽  
Human Cervical Cancer Cell ◽  
Line Hela ◽  
Human Cervical Cancer

Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line

1996 ◽  
Vol 102 (1-2) ◽  
pp. 173-181 ◽  
Author(s):  
Cheng-Yang Chou ◽  
Yen-Hui Chen ◽  
Ching-Cherng Tzeng ◽  
Yueh-Chin Cheng ◽  
Chin-Feng Chang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
P53 Mutation ◽  
Cervical Cancer Cell Line ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer
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