Synthesis of 3-(2-(subsituted-(trifluoromethyl)phenylamino)acetyl)-2H-chromen-2-one derivatives as new anticancer agents

Under solvent free conditions and in presence of a base 3-(2-(subsituted-(trifluoromethyl)phenylamino)acetyl)-2H-chromen-2-one derivatives were synthesized by grinding technique. Structural investigations were carried out with IR studies, HRMS, 1HNMR and 13CNMR. The compounds were checked for their in vitro anticancer activities against three different human cancer cell lines viz human breast cancer cell line (MCF-7), human cervical cancer cell line (HeLa) and human oral squamous cell carcinoma (SCC-40) using SRB method. All the title compounds showed low toxicity towards non-malignant PBMC cells indicating their tumour selectivity. The compounds exhibited good in vitro anti-proliferative potency at lower concentrations against HeLa and MCF-7 cell lines and remain moderately active against SCC-40.

Antifungal, Antibacterial, and Cytotoxic Activities of Silver Nanoparticles Synthesized from Aqueous Extracts of Mace-Arils of Myristica fragrans

In the present study, mace-mediated silver nanoparticles (mace-AgNPs) were synthesized, characterized, and evaluated against an array of pathogenic microorganisms. Mace, the arils of Myristica fragrans, are a rich source of several bioactive compounds, including polyphenols and aromatic compounds. During nano synthesis, the bioactive compounds in mace aqueous extracts serve as excellent bio reductants, stabilizers, and capping agents. The UV-VIS spectroscopy of the synthesized NPs showed an intense and broad SPR absorption peak at 456 nm. Dynamic light scattering (DLS) analysis showed the size with a Z average of 50 nm, while transmission electron microscopy (TEM) studies depicted the round shape and small size of the NPs, which ranged between 5–28 nm. The peaks related to important functional groups, such as phenols, alcohols, carbonyl groups, amides, alkanes and alkenes, were obtained on a Fourier-transform infrared spectroscopy (FTIR) spectrum. The peak at 3 keV on the energy dispersive X-ray spectrum (EDX) validated the presence of silver (Ag). Mace-silver nanoparticles exhibited potent antifungal and antibacterial activity against several pathogenic microorganisms. Additionally, the synthesized mace-AgNPs displayed an excellent cytotoxic effect against the human cervical cancer cell line. The mace-AgNPs demonstrated robust antibacterial, antifungal, and cytotoxic activity, indicating that the mace-AgNPs might be used in the agrochemical industry, pharmaceutical industry, and biomedical applications. However, future studies to understand its mode of action are needed.

Assessing the antiumer and anti-inflammatory potentials of Calotropis procera latex

Since time immemorial medicinal plants have been used in healthcare to treat different human disease. The ideals of medicinal plants are highlighted due to the utility of the common-factor approach to engage other health promoters in propagating. One of these medicinal plants is Calotropis procera which is a species of tree in the family Apocynaceae. Calotropis procera is widespread in tropical Africa, including the Indian Ocean islands and the northern parts of South Africa. The latex is toxic and can cause rash, blisters and serious inflammations in sensitive persons and it may lead to blindness. Several side effects may be caused due to ingestion large doses of latex like burning in the throat, irritation of the stomach, nausea, vomiting, diarrhea, tremors, vertigo and convulsions. This study aimed to evaluate burns healing effects and inhibitory concentration IC50 of Calotropis procera latex on cervical cancer cell line, SiHa. Cytotoxicity analysis was performed by MTT assay in addition to determine the burns healing effect of the latex by determining the day requiring to heal the burn skin of albino male mice. The results of burns healing effects declared that the burns required 12 days to heal in comparison with positive (sliver sulfadiazine) and negative control which required 16 and 18 days for healing respectively. Also, the results revealed that the IC50 of latex was 146.8 % in comparison of ambiguous percentage of normal cell line WRL68 with reduction in cancer cell viability ranging from 95.87± 0.20to 52.35 ±3.31 for 6.2 to 400 µg\ml respectively.

The effect of Chaihu-shugan-san on cytotoxicity induction and PDGF gene expression in cervical cancer cell line HeLa in the presence of paclitaxel +cisplatin

Chaihu-shugan-san, as a traditional Chinese herbal formula, is composed of seven different herbs. This medicine can treat cancer due to its antioxidant compounds. In this study, the effect of Chaihu-shugan-san was considered on cytotoxicity induction and PDGF gene expression in cervical cancer cell line HeLa at different concentrations and at different times, by the MTT method. Paclitaxel + cisplatin were used as a control in this study. The expression of the PDGF gene was quantitatively evaluated in treated cells by real-time PCR, and a generalized linear model was used to evaluate the effect of the medicine, and Duncan's multiple range tests were used to evaluate the data. The results of the MTT test showed that Chaihu-shugan-san had antitumor properties in different concentrations, but there was a significant difference between this medicine and paclitaxel +cisplatin. Also, examination of gene expression showed that this medicine reduced the expression of the PDGF gene in the HeLa cancer cell line (P ? 0.04). Therefore, Chaihu-shugan-san could be suggested as an effective factor in preventing the growth of cervical cancer cells and controlling angiogenic factors that play an important role in the metastasis of cancerous tumors.

The Extensive and Expensive Impacts of HEp-2 [HeLa], Intestine 407 [HeLa], and Other False Cell Lines in Journal Publications

Cell lines are essential models for biomedical research. However, they have a common and important problem that needs to be addressed. Cell lines can be misidentified, meaning that they no longer correspond to the donor from whom the cells were first obtained. This problem may arise due to cross-contamination: the accidental introduction of cells from another culture. The contaminant, which is often a rapidly dividing cell line, will overgrow and replace the original culture. The end result is a false cell line, also known as a misidentified or imposter cell line. False cell lines may come from an entirely different species, tissue, or cell type than the original donor. If undetected, false cell lines produce unreliable and irreproducible results that pollute the biomedical literature and threaten the development of reliable drug discovery and meaningful patient treatments. The goal of this study was to ascertain how widespread this problem is and how it affects the literature, as well as to estimate how much funding has been used to produce pools of scientific literature of questionable value. We focus on HEp-2 [HeLa] and Intestine 407 [HeLa], two false cell lines that are widely used in the scientific literature but were shown to be cross-contaminated in 1967. These two cell lines have been used in 8497 and 1397 published articles and extensively described as laryngeal cancer and normal intestine, respectively, rather than their true identity: the cervical cancer cell line HeLa. Discussed are tools, approaches, and resources that can address this issue—both retrospectively and prospectively.

Multifaceted bioproperties of Streptomyces bacillaris ANS2 isolated from Andaman and Nicobar Islands, India

The rich warehouse called marine actinobacteria has enticed researchers for several decades. The assessment of their metabolites has evidenced several diverse and multiple biological properties vacillating from antibacterial, antituberculosis, antioxidant, anticancer to mosquitocidal. Therefore, the present study has been aimed to reconnoitre the isolation and portrayal of the promising marine actinobacteria named Streptomyces bacillaris ANS2 isolated from the mangrove sediment collected from Andaman and Nicobar islands. The ethyl acetate extract of ANS2 showed broad spectrum activity against various pathogens including Escherichia coli (18.0±0.3 mm), Salmonella paratyphi (16.0±0.6 mm) and Klebsiella pneumonia (14.8±0.7 mm). It showed maximum activity against drug sensitive strain of Mycobacterium tuberculosis (98.2±0.36%) followed by M. tuberculosis MDR strain (97.04±1.32%) and M. tuberculosis H37Rv (91.80±0.45%). The antioxidant activity by DPPH assay of ANS2 showed 29.9±0.38 - 61.35±0.78 % free radical scavenging at 100μg/ml - 500μg/ml as compared to the standard ascorbic acid which showed 96±1.0% at 100μg/ml concentration. The anticancer activity showed 88.12±0.9% and 89.5±0.26% inhibition against HT 29 (colon cancer) and HeLa (cervical cancer) cell line at 1000μg/ml concentration. The maximum larval mortality was recorded on Culex quinquefasciatus(LC50 =1100.134 μg/ml and r2= 0.99) and Aedes aegypti (LC50 =690.620 μg/ml and r2= 0.99).The biochemical, morphological and 16S rRNA gene analysis study clearly confirmed that the promising strain belonged to the genus Streptomyces bacillaris and the phylogenetic tree confirmed it as S. bacillaris. Results showed that S.bacillaris ANS2 showed multidimensional bioproperties.

Multifunctional silica nanocomposites prime tumoricidal immunity for efficient cancer immunotherapy

The tumor immune microenvironment (TIME) has been demonstrated to be the main cause of cancer immunotherapy failure in various malignant tumors, due to poor immunogenicity and existence of immunosuppressive factors. Thus, establishing effective treatments for hostile TIME remodeling has considerable potential to enhance immune response rates for durable tumor growth retardation. This study aims to develop a novel nanocomposite, polyethyleneimine-modified dendritic mesoporous silica nanoparticles loaded with microRNA-125a (DMSN-PEI@125a) to synergistically enhance immune response and immunosuppression reversion, ultimately generating a tumoricidal environment. Our results showed that DMSN-PEI@125a exhibited excellent ability in cellular uptake by murine macrophages and the cervical cancer cell line TC-1, repolarization of tumor associated macrophages (TAMs) to M1 type in a synergistic manner, and promotion of TC-1 immunogenic death. Intratumor injection of DMSN-PEI@125a facilitated the release of more damage-related molecular patterns and enhanced the infiltration of natural killer and CD8+ T cells. Meanwhile, repolarized TAMs could function as a helper to promote antitumor immunity, thus inhibiting tumor growth in TC-1 mouse models in a collaborative manner. Collectively, this work highlights the multifunctional roles of DMSN-PEI@125a in generating an inflammatory TIME and provoking antitumor immunity, which may serve as a potential agent for cancer immunotherapy.