scholarly journals Role of hypoxia and growth and differentiation factor-5 on differentiation of human mesenchymal stem cells towards intervertebral nucleus pulposus-like cells

2011 ◽  
Vol 21 ◽  
pp. 533-547 ◽  
Author(s):  
JV Stoyanov ◽  
◽  
B Gantenbein-Ritter ◽  
A Bertolo ◽  
N Aebli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Differentiation Factor

Magnetic field assisted stem cell differentiation – role of substrate magnetization in osteogenesis

2015 ◽  
Vol 3 (16) ◽  
pp. 3150-3168 ◽  
Author(s):  
Sunil Kumar Boda ◽  
Greeshma Thrivikraman ◽  
Bikramjit Basu
Keyword(s):  
Magnetic Field ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Bone Tissue Engineering ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation

Substrate magnetization as a tool for modulating the osteogenesis of human mesenchymal stem cells for bone tissue engineering applications.

scholarly journals The role of hesperetin on osteogenesis of human mesenchymal stem cells and its function in bone regeneration

Oncotarget ◽  
2017 ◽  
Vol 8 (13) ◽  
pp. 21031-21043 ◽  
Author(s):  
Deting Xue ◽  
Erman Chen ◽  
Wei Zhang ◽  
Xiang Gao ◽  
Shengdong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Human Mesenchymal Stem Cells

Su1793 The Protective Role of Human Mesenchymal Stem Cells in Acute Pancreatitis in Mice

2013 ◽  
Vol 144 (5) ◽  
pp. S-478
Author(s):  
Michael Gonzales ◽  
Xuxia Gao ◽  
Yanna Cao ◽  
Hasen Xue ◽  
Judith Aronson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Pancreatitis ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Protective Role

scholarly journals Effect of lentivirus-mediated growth and differentiation factor-5 transfection on differentiation of rabbit nucleus pulposus mesenchymal stem cells

2022 ◽  
Vol 27 (1) ◽  
Author(s):  
Kun Zhu ◽  
Rui Zhao ◽  
Yuchen Ye ◽  
Gang Xu ◽  
Changchun Zhang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Lentiviral Vector ◽  
The Other ◽  
Cell Phenotype ◽  
Mrna Levels ◽  
Qrt Pcr ◽  
Differentiation Factor ◽  
Gdf5 Gene

Abstract Background Intervertebral disc degeneration (IDD) is a natural progression of age-related processes. Associated with IDD, degenerative disc disease (DDD) is a pathologic condition implicated as a major cause of chronic lower back pain, which can have a severe impact on the quality of life of patients. As degeneration progression is associated with elevated levels of inflammatory cytokines, enhanced aggrecan and collagen degradation, and changes in the disc cell phenotype. The purpose of this study was to investigate the biological and cytological characteristics of rabbit nucleus pulposus mesenchymal stem cells (NPMSCs)—a key factor in IDD—and to determine the effect of the growth and differentiation factor-5 (GDF5) on the differentiation of rabbit NPMSCs transduced with a lentivirus vector. Methods An in vitro culture model of rabbit NPMSCs was established and NPMSCs were identified by flow cytometry (FCM) and quantitative real-time PCR (qRT-PCR). Subsequently, NPMSCs were randomly divided into three groups: a transfection group (the lentiviral vector carrying GDF5 gene used to transfect NPMSCs); a control virus group (the NPMSCs transfected with an ordinary lentiviral vector); and a normal group (the NPMSCs alone). FCM, qRT-PCR, and western blot (WB) were used to detect the changes in NPMSCs. Results The GDF5-transfected NPMSCs displayed an elongated shape, with decreased cell density, and significantly increased GDF5 positivity rate in the transfected group compared to the other two groups (P < 0.01). The mRNA levels of Krt8, Krt18, and Krt19 in the transfected group were significantly higher in comparison with the other two groups (P < 0.01), and the WB results were consistent with that of qRT-PCR. Conclusions GDF5 could induce the differentiation of NPMSCs. The lentiviral vector carrying the GDF5 gene could be integrated into the chromosome genome of NPMSCs and promoted differentiation of NPMSCs into nucleus pulposus cells. Our findings advance the development of feasible and effective therapies for IDD.

scholarly journals Role of Wnt-5a in the Determination of Human Mesenchymal Stem Cells into Preadipocytes

2009 ◽  
Vol 285 (9) ◽  
pp. 6170-6178 ◽  
Author(s):  
Roman Bilkovski ◽  
Dominik M. Schulte ◽  
Frank Oberhauser ◽  
Matthias Gomolka ◽  
Michael Udelhoven ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

scholarly journals Three-dimensional hypoxic culture of human mesenchymal stem cells encapsulated in a photocurable, biodegradable polymer hydrogel: A potential injectable cellular product for nucleus pulposus regeneration

2014 ◽  
Vol 10 (8) ◽  
pp. 3463-3474 ◽  
Author(s):  
Deepak Kumar ◽  
Irini Gerges ◽  
Margherita Tamplenizza ◽  
Cristina Lenardi ◽  
Nicholas R. Forsyth ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Biodegradable Polymer ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Polymer Hydrogel

Critical role of phosphoinositide 3-kinase cascade in adipogenesis of human mesenchymal stem cells

2007 ◽  
Vol 310 (1-2) ◽  
pp. 11-18 ◽  
Author(s):  
Weihua Yu ◽  
Zhenguang Chen ◽  
Jinli Zhang ◽  
Lirong Zhang ◽  
Hui Ke ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Critical Role ◽  
Human Mesenchymal Stem Cells ◽  
Kinase Cascade ◽  
Phosphoinositide 3 Kinase

scholarly journals BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype

2015 ◽  
Vol 16 (9) ◽  
pp. 20344-20359 ◽  
Author(s):  
Xiaopeng Zhou ◽  
Yiqing Tao ◽  
Chengzhen Liang ◽  
Yujie Zhang ◽  
Hao Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells

Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogels

2015 ◽  
Vol 104 (1) ◽  
pp. 165-177 ◽  
Author(s):  
Huizi A. Lin ◽  
Michelle S. Gupta ◽  
Devika M. Varma ◽  
M. Lane Gilchrist ◽  
Steven B. Nicoll
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Crosslinking Density

scholarly journals Role of Slug transcription factor in human mesenchymal stem cells

2012 ◽  
Vol 16 (4) ◽  
pp. 740-751 ◽  
Author(s):  
Elena Torreggiani ◽  
Gina Lisignoli ◽  
Cristina Manferdini ◽  
Elisabetta Lambertini ◽  
Letizia Penolazzi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Transcription Factor ◽  
Human Mesenchymal Stem Cells
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