Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogels

2015 ◽  
Vol 104 (1) ◽  
pp. 165-177 ◽  
Author(s):  
Huizi A. Lin ◽  
Michelle S. Gupta ◽  
Devika M. Varma ◽  
M. Lane Gilchrist ◽  
Steven B. Nicoll
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Crosslinking Density

scholarly journals Three-dimensional hypoxic culture of human mesenchymal stem cells encapsulated in a photocurable, biodegradable polymer hydrogel: A potential injectable cellular product for nucleus pulposus regeneration

2014 ◽  
Vol 10 (8) ◽  
pp. 3463-3474 ◽  
Author(s):  
Deepak Kumar ◽  
Irini Gerges ◽  
Margherita Tamplenizza ◽  
Cristina Lenardi ◽  
Nicholas R. Forsyth ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Biodegradable Polymer ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Polymer Hydrogel

scholarly journals BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype

2015 ◽  
Vol 16 (9) ◽  
pp. 20344-20359 ◽  
Author(s):  
Xiaopeng Zhou ◽  
Yiqing Tao ◽  
Chengzhen Liang ◽  
Yujie Zhang ◽  
Hao Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells

scholarly journals Role of hypoxia and growth and differentiation factor-5 on differentiation of human mesenchymal stem cells towards intervertebral nucleus pulposus-like cells

2011 ◽  
Vol 21 ◽  
pp. 533-547 ◽  
Author(s):  
JV Stoyanov ◽  
◽  
B Gantenbein-Ritter ◽  
A Bertolo ◽  
N Aebli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Differentiation Factor

scholarly journals Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

2011 ◽  
Vol 21 (S6) ◽  
pp. 826-838 ◽  
Author(s):  
Alessandro Bertolo ◽  
Marco Mehr ◽  
Niklaus Aebli ◽  
Martin Baur ◽  
Stephen J. Ferguson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Differentiation

Roles of FGF-2 and TGF-beta/FGF-2 on differentiation of human mesenchymal stem cells towards nucleus pulposus-like phenotype

2014 ◽  
Vol 33 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Xiaopeng Zhou ◽  
Yiqing Tao ◽  
Jin Wang ◽  
Chengzhen Liang ◽  
Jun Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Tgf Beta

scholarly journals Non-invasive Raman Spectroscopy and Quantitative Real-Time PCR Distinguish Among Undifferentiated Human Mesenchymal Stem Cells and Redifferentiated Nucleus Pulposus Cells and Chondrocytes In Vitro

2017 ◽  
Vol 11 (1) ◽  
pp. 72-84
Author(s):  
Franziska Ehlicke ◽  
Natascha Köster ◽  
Denise Salzig ◽  
Peter Czermak
Keyword(s):  
Stem Cells ◽  
Raman Spectroscopy ◽  
Mesenchymal Stem Cells ◽  
Real Time ◽  
Nucleus Pulposus ◽  
Real Time Pcr ◽  
Human Mesenchymal Stem Cells ◽  
Quantitative Real Time Pcr ◽  
Non Invasive ◽  
Differentiation Status

Background:The most common cause of lower back pain is the pathological degeneration of the nucleus pulposus (NP). Promising NP regeneration strategies involving human mesenchymal stem cells (hMSCs) would require specific markers to confirm successful differentiation into the NP lineage and to distinguish the articular cartilage (AC).Objective:We sought specific NP mRNA markers that are upregulated in native NP cells but not in dedifferentiated NP cells, undifferentiated hMSCs or chondrocytes. We also considered the suitability of non-invasive Raman spectroscopy to distinguish among these classes of cells.Method:We used quantitative real-time PCR and Raman spectroscopy to analyse undifferentiated hMSCs in monolayers and embedded in hydrogels, and compared the results with dedifferentiated and redifferentiated human NP and AC cells.Results:The redifferentiation of NP cells induced the expression of annexin A3 (ANXA3), collagen type II (COL2) and proteoglycan mRNAs, whereas the redifferentiation of AC cells only induced proteoglycan expression. Redifferentiated NP cells expressed higher levels ofANXA3,COL2, paired box 1 (PAX1) andOCT4mRNA than redifferentiated AC cells. Redifferentiated NP cells and undifferentiated hMSC-TERT cells expressed similar amount ofOCT4mRNA, indicating that onlyANXA3,COL2andPAX1are promising markers for redifferentiated NP cells. Raman spectra clearly differed among the three cell types and highlighted their differentiation status.Conclusion:We recommendANXA3,COL2andPAX1as markers to determine the success of hMSC-based differentiation to regenerate NP cells. Raman spectroscopy can be used to determine cell type and differentiation status especially in the context of clinical trials.

99. Human Mesenchymal Stem Cells Differentiate into NP-like Cells When Cultured on Allograft Nucleus Pulposus Scaffolds

2008 ◽  
Vol 8 (5) ◽  
pp. 50S-51S
Author(s):  
Frank Phillips ◽  
Monica Susilo ◽  
Eric Nauman ◽  
David Van Sickle ◽  
Bret Ferree
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells

scholarly journals Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells

2011 ◽  
Vol 13 (3) ◽  
pp. R81 ◽  
Author(s):  
Devina Purmessur ◽  
Rachel M Schek ◽  
Rosalyn D Abbott ◽  
Bryan A Ballif ◽  
Karolyn E Godburn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Conditioned Media
Sign in / Sign up

Export Citation Format

Share Document