Effectiveness of thidiazuron and naphthaleneacetic acid during adventitious shoot bud induction and plant regeneration from leaf explants of rhinacanthus Nasutus (l.) Kurz

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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In vitro shoot regeneration of boldo from leaf explants

Ciência Rural ◽

10.1590/s0103-84782010005000173 ◽

2010 ◽

Vol 40 (10) ◽

pp. 2210-2213

Author(s):

Monalize Salete Mota ◽

Juliana de Magalhães Bandeira ◽

Eugenia Jacira Bolacel Braga ◽

Valmor João Bianchi ◽

José Antonio Peters

Keyword(s):

Shoot Regeneration ◽

Leaf Explants ◽

Shoot Development ◽

High Potential ◽

Naphthaleneacetic Acid ◽

Regeneration System ◽

Bud Induction ◽

Molecular Studies ◽

In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

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Establishment of a Rapid and Efficient Micropropagation System for Succulent Plant Haworthia turgida Haw.

HortScience ◽

10.21273/hortsci12056-17 ◽

2017 ◽

Vol 52 (9) ◽

pp. 1278-1282 ◽

Cited By ~ 4

Author(s):

Boling Liu ◽

Hongzhou Fang ◽

Chaorong Meng ◽

Ming Chen ◽

Qingdong Chai ◽

...

Keyword(s):

Callus Induction ◽

Adventitious Shoots ◽

Germplasm Conservation ◽

Leaf Explants ◽

Adventitious Shoot ◽

Induction Medium ◽

Naphthaleneacetic Acid ◽

Root Induction ◽

Ms Medium ◽

Succulent Plant

In the present study, the effect of plant growth regulators (PGRs) on callus regeneration, adventitious shoot differentiation, and root formation of Haworthia turgida Haw. was investigated. The greatest callus induction percentage (95.6%) was achieved with leaf explants inoculated on Murashige and Skoog (MS) medium with 1.0 mg·L−1 6-benzyladenine (BA) and 0.1 mg·L−1 1-naphthaleneacetic acid (NAA), and this callus induction medium supplemented with 2.5 mg·L−1 thidiazuron (TDZ) was optimal for callus proliferation. The maximum number of shoots (25.7) was obtained when the callus was cultured on MS medium supplemented with 1.0 mg·L−1 BA and 0.2 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest number of roots per shoot (6.2) and highest rooting frequency (82.0%) were obtained when adventitious shoots were inoculated on MS medium with 0.05 mg·L−1 NAA. Regenerated plantlets were transferred to a mixture of vermiculite and soil and acclimated in a greenhouse. The survival rate of the transplanted plantlets was about 91.6%. The rate of ex vitro rooting was 83.3%, indicating that this technique is effective for root induction in H. turgida. This study has established a rapid and efficient micropropagation system that can be beneficial for commercial cultivation and germplasm conservation of H. turgida.

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Tryptophan metabolism and evaluation of morphological, biochemical and molecular variations in a field grown plant population derived via direct adventitious shoot bud regeneration from pre-plasmolysed leaves of Catharanthus roseus

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-015-0841-0 ◽

2015 ◽

Vol 123 (2) ◽

pp. 357-375 ◽

Cited By ~ 3

Author(s):

Priyanka Verma ◽

Shamshad Ahmad Khan ◽

Ajay Kumar Mathur ◽

Alka Srivastava ◽

Karuna Shanker

Keyword(s):

Catharanthus Roseus ◽

Adventitious Shoot ◽

Plant Population ◽

Tryptophan Metabolism ◽

Bud Regeneration ◽

Shoot Bud ◽

Shoot Bud Regeneration ◽

Adventitious Shoot Bud ◽

Grown Plant

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REGENERATION OF TREMA ORIENTALIS (BLUME) LINN.: EFFECT OF GROWTH REGULATORS, CULTURE CONDITIONS, AND AGE AND SOURCE OF EXPLANTS

Israel Journal of Plant Sciences ◽

10.1080/07929978.1995.10676625 ◽

1995 ◽

Vol 43 (4) ◽

pp. 391-395 ◽

Cited By ~ 1

Author(s):

G.R. Rout ◽

S. Samantaray ◽

P. Das

Keyword(s):

Growth Regulators ◽

Leaf Explants ◽

Naphthaleneacetic Acid ◽

Mature Trees ◽

Regeneration Rate ◽

Bud Regeneration ◽

Shoot Bud ◽

Shoot Bud Regeneration ◽

Trema Orientalis

Optimal conditions for high frequency shoot bud regeneration from leaf callus of Trema orientalis (Blume) Linn. were studied. The regeneration rate was controlled by the growth regulators, the age and the source of the explants, and the illumination conditions. Irrespective of illumination conditions, shoot bud regeneration was achieved only in media containing benzyladenine (BA) + α-naphthaleneacetic acid (NAA) combinations, with the best results being obtained in the presence of 2.5 mg/1 BA and 0.25–0.5 mg/1 NAA. The morphogenic response was less frequent in the calluses derived from leaf explants of the mature trees compared to those of the in vitro-grown seedlings. The rate of shoot bud regeneration was more pronounced in the cultures maintained for 4 weeks in the light (16-h photoperiod) than the cultures incubated in the dark. Regenerated shoots were rooted on the medium containing 1/2 strength basal Murashige and Skoog (MS) salts supplemented with 0.01 mg/1 NAA or indole-3-butyric acid (IBA). The rooted plantlets were established in the greenhouse.

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