scholarly journals Enhancing microaerobic plasmid DNA production by chromosomal expression of Vitreoscilla hemoglobin in E. coli

Authorea ◽  
2020 ◽  
Author(s):  
Karim Ja n ◽  
Daniela Velazquez ◽  
Juan Sigala Alanis ◽  
Alvaro Lara
Keyword(s):  
Plasmid Dna ◽  
Vitreoscilla Hemoglobin ◽  
E Coli ◽  
Dna Production

Enhancing microaerobic plasmid DNA production by chromosomal expression of Vitreoscilla hemoglobin in E. coli

2020 ◽  
pp. 107862
Author(s):  
Karim E. Jaén ◽  
Daniela Velazquez ◽  
Juan-Carlos Sigala ◽  
Alvaro R. Lara
Keyword(s):  
Plasmid Dna ◽  
Vitreoscilla Hemoglobin ◽  
E Coli ◽  
Dna Production

scholarly journals De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production

2012 ◽  
Vol 97 (2) ◽  
pp. 611-620 ◽  
Author(s):  
Geisa A. L. Gonçalves ◽  
Duarte M. F. Prazeres ◽  
Gabriel A. Monteiro ◽  
Kristala L. J. Prather
Keyword(s):  
Plasmid Dna ◽  
De Novo ◽  
E Coli ◽  
Dna Production

One-Step Preparation of Competent E. coli: Transformation and Storage of Bacterial Cells in the Same Solution

2021 ◽  
Vol 2021 (11) ◽  
pp. pdb.prot101212 ◽  
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  
Escherichia Coli ◽  
Convenient Method ◽  
Plasmid Dna ◽  
Transformation Efficiency ◽  
Bacterial Cells ◽  
E Coli ◽  
One Step ◽  
And Storage

This protocol describes a convenient method for the preparation, use, and storage of competent Escherichia coli. The reported transformation efficiency of this method is ∼5 × 107 transformants/µg of plasmid DNA.

Preliminary studies on medium and fermentation strategy design for plasmid DNA production

2007 ◽  
Vol 131 (2) ◽  
pp. S166-S167
Author(s):  
Filomena Silva ◽  
Luís Passarinha ◽  
Fani Sousa ◽  
João Queiróz ◽  
Fernanda Domingues
Keyword(s):  
Plasmid Dna ◽  
Dna Production ◽  
Fermentation Strategy ◽  
Strategy Design
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