Lipopolysaccharide-binding protein, neopterin and interferon-gamma as indices of inflammation activity in patients with acute brucellosis

Brucellosis is characterized by nonspecific clinical manifestations, the possibility of subclinical flow, the development of relapses and chronic course. Currently, there are nolaboratory criteria to assess the activity of inflammation in brucellosis, the effectiveness of the therapy, predict the outcome of the disease and the risks of recurrence. Available in clinical practice, laboratory tests to assess inflammation, in particular, erythrocyte sedimentation rate, C-reactive protein, leukocyte level, with brucellosis infection are almost not informative. An important role in the development of the cellular immune response against brucella is played by interferon-γ, lipopolysaccharide-binding protein and neopterin. The aim of the study was to determine the level of lipopolysaccharide-binding protein, neopterin and interferon-γ,in the serum of patients with acute form of brucellosis beforeand after antibacterial treatment. When studying the blood of patients with acute brucellosis before and after therapy, the indices of neopterin, lipopolysaccharide-binding protein and interferon-γ were significantly higher than normalvalues. The obtained results testify to the persisting active inflammation and the formation of a chronic brucellosis. Determination of the level of lipopolysaccharide-binding protein, neopterin and interferon-γ in the blood of patients withbrucellosis can be used as markers of inflammation and in monitoring the effectiveness of antibacterial therapy.

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Lipopolysaccharide-Binding Protein: A New Biomarker for Infectious Endocarditis?

Clinical Chemistry ◽

10.1373/clinchem.2008.106195 ◽

2009 ◽

Vol 55 (2) ◽

pp. 295-304 ◽

Cited By ~ 18

Author(s):

Tanja Vollmer ◽

Cornelia Piper ◽

Knut Kleesiek ◽

Jens Dreier

Keyword(s):

Binding Protein ◽

Molecular Genetic ◽

Protein A ◽

Response To Therapy ◽

Infectious Endocarditis ◽

Therapeutic Monitoring ◽

Lipopolysaccharide Binding Protein ◽

Reactive Protein ◽

Wbc Count ◽

Lipopolysaccharide Binding

Abstract Background: Infectious endocarditis (IE) is a bacterial infection of the endocardium. Diagnosis is based on results obtained from echocardiography, blood cultures, and molecular genetic screening for bacteria and on data for inflammatory markers such as the leukocyte (WBC) count and the C-reactive protein (CRP) concentration. The aim of the present study was to evaluate lipopolysaccharide-binding protein (LBP) as a supportive biomarker for the diagnosis and therapeutic monitoring of IE. Methods: We measured LBP and CRP concentrations and WBC counts in 57 IE patients at hospital admission, 40 patients with noninfectious heart valve diseases (HVDs), and 55 healthy blood donors. The progression of these 3 markers and the influence of cardiac surgery on them were evaluated in 29 IE patients and 21 control patients. Results: Serum LBP concentrations were significantly higher in IE patients [mean (SD), 33.41 (32.10) mg/L] compared with HVD patients [6.67 (1.82) mg/L, P < 0.0001] and healthy control individuals [5.61 (1.20) mg/L]. The progression in the LBP concentration during therapy of IE patients correlated with the changes in the CRP concentration. The 2 markers were equally influenced by antibiotic treatment and surgical intervention. Conclusions: Serial LBP measurement may provide an effective and useful tool for evaluating the response to therapy in IE patients. We found a strong correlation between LBP and CRP concentrations; LBP has a tendency to increase earlier in cases of reinfection.

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High-mobility group box-1 protein, lipopolysaccharide-binding protein, interleukin-6 and C-reactive protein in children with community acquired infections and bacteraemia: a prospective study

BMC Infectious Diseases ◽

10.1186/1471-2334-10-28 ◽

2010 ◽

Vol 10 (1) ◽

Cited By ~ 15

Author(s):

Jana Pavare ◽

Ilze Grope ◽

Imants Kalnins ◽

Dace Gardovska

Keyword(s):

Prospective Study ◽

Interleukin 6 ◽

Binding Protein ◽

High Mobility ◽

High Mobility Group ◽

C Reactive Protein ◽

Lipopolysaccharide Binding Protein ◽

Reactive Protein ◽

A Prospective Study ◽

Lipopolysaccharide Binding

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Lipopolysaccharide binding protein, interleukin-6 and C-reactive protein in acute gastrointestinal infections: value as biomarkers to reduce unnecessary antibiotic therapy

Infection ◽

10.1007/s15010-011-0117-5 ◽

2011 ◽

Vol 39 (4) ◽

pp. 327-331 ◽

Cited By ~ 19

Author(s):

C. Elsing ◽

S. Ernst ◽

N. Kayali ◽

W. Stremmel ◽

S. Harenberg

Keyword(s):

Antibiotic Therapy ◽

Interleukin 6 ◽

Binding Protein ◽

C Reactive Protein ◽

Lipopolysaccharide Binding Protein ◽

Gastrointestinal Infections ◽

Reactive Protein ◽

Lipopolysaccharide Binding

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The Use of Selective Hemosorption of Lipopolysaccharides in the Complex Treatment of Sepsis

Russian Sklifosovsky Journal Emergency Medical Care ◽

10.23934/2223-9022-2020-9-2-201-209 ◽

2020 ◽

Vol 9 (2) ◽

pp. 201-209

Author(s):

G. V. Bulava ◽

S. I. Rey ◽

G. A. Berdnikov ◽

O. V. Nikitina ◽

A. K. Shabanov ◽

...

Keyword(s):

Interleukin 6 ◽

Binding Protein ◽

Polymyxin B ◽

Interleukin 10 ◽

C Reactive Protein ◽

Lipopolysaccharide Binding Protein ◽

Reactive Protein ◽

Endotoxin Activity ◽

Blood Cytokines ◽

Lipopolysaccharide Binding

Background Sepsis and septic shock are formidable and complications in surgery with mortality 20–50%. In the pathogenesis of sepsis, a significant role belongs to bacterial endotoxin (LPS - liposaccharide).Aim of study Assessment of the efficacy of selective lipopolysaccharides hemosorption (SLH) in treatment of sepsis.Material and methods We examined 65 patients with developed sepsis or suspected presence of gram-negative infection. Patients were retrospectively divided into two groups. In Group 1, 27 patients received Polymyxin B hemoperfusion using Toraymyxin cartridges. In Group 2 (38 patients), adsorber Alteco (LPSA) was used.Results It was established that 28-day mortality was 11.1% in Polymyxin group and 28.9% in LPS group A, p = 0.091, 60-day mortality was 33.3 and 55.3%, respectively (p=0.065). The use of SLH contributed to a decrease in the activity of endotoxin (EAA) from 0.52 (0.39; 0.65) to 0.40 (0.36; 0.57) EU (p=0.330) in Polymyxin group and from 0.59 ( 0.42; 0.72) to 0.54 (0.40; 0.81) EU ( p = 0.981) in the LPS-A group. At the same time, the level of procalcitonin (PCT) in the blood statistically significantly decreased from 8.4 (3.6; 29.0) to 4.8 (1.9; 36.3) ng/ml (p=0.0117) only in the LPS-A group. The level of C-reactive protein (CRB) in the blood statistically significantly decreased only in the Polymyxin group, from 205 (154; 264) to 162 (106; 202) mg/L (p<0.001). After SPH procedures, there was a tendency to a decrease in the level of blood cytokines in both groups. Conclusion 1. The trend of better survival among patients was noted during hemoperfusion when using Polymyxin B in comparison with the results of adsorption of lipopolysaccharide with Alteco cartridges: so, 28-day mortality was 11.1 and 28.9%, respectively (statistically not significant).2. As a result, the procedure of selective lipopolysaccharides hemosorbtion on hemosorbents with Polymyxin B in blood significantly decreased level of C-reactive protein (21%), there was statistically insignificant decrease in the level of endotoxin activity (23.1%), lipopolysaccharide binding protein (21.6%), procalcitonin (2.4 times), presepsin (20%), as well as the level of interleukin-6 (3.4 times) and interleukin-10 (1.6 times) . Adsorption of lipopolysaccharide with Alteco cartridges leads to a statistically significant reduction of procalcitonin in blood (1.8 times), and statistically insignificant decrease of: endotoxin activity (9.3%), lipopolysaccharide binding protein (28.6%), interleukin-6 (3.8 times), interleukin-10 (7.1 times) and soluble receptor to interleukin-2 (2.2 times).

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