scholarly journals Assessment of the genotoxic potential of Lake Skadar sediments using Ames test and comet assay on the fish cell line RTL-W1

2012 ◽  
Vol 64 (1) ◽  
pp. 249-256 ◽  
Author(s):  
A. Perovic ◽  
Svetlana Perovic ◽  
L. Erdinger ◽  
H. Hollert
Keyword(s):  
Comet Assay ◽  
Cell Line ◽  
Ames Test ◽  
In Vitro Tests ◽  
Fish Cell ◽  
Genotoxic Potential ◽  
Permanent Cell ◽  
Organic Fractions ◽  
Lake Skadar

In this study we evaluated the genotoxic potential of surface sediment extracts of Lake Skadar using a combination of two in vitro tests: the Comet assay on the fibroblast-like permanent cell line RTL-W1, and the Ames test on the strain Salmonella typhimurium TA98. The obtained results show that both tests were successful in determining the genotoxic potential in the sediment organic fractions. They possess enough sensitivity to detect early warning signals and evaluate the genotoxic potential in sediments of the Lake. The genotoxic potential was recorded and compared in the sediment samples from different locations on the Lake Skadar.

scholarly journals Comparison of methods used for evaluation of mutagenicity/genotoxicity of model chemicals - parabens

2020 ◽  
pp. S661-S679
Author(s):  
J Chrz ◽  
B Hošíková ◽  
L Svobodová ◽  
D Očadlíková ◽  
H Kolářová ◽  
...  
Keyword(s):  
Comet Assay ◽  
Chromosomal Aberration ◽  
Water Solubility ◽  
Consumer Products ◽  
Weight Of Evidence ◽  
In Vitro Tests ◽  
Genotoxic Potential ◽  
Chromosomal Aberration Test

Growing worldwide efforts to replace (reduce) animal testing and to improve alternative in vitro tests which may be more efficient in terms of both time, cost and scientific validity include also genotoxicity/mutagenicity endpoints. The aim of the review article was to summarize currently available in vitro testing approaches in this field, their regulatory acceptance and recommended combinations for classification of chemicals. A study using the combination of Comet Assay performed on two cell lines and the Chromosomal Aberration test on human peripheral lymphocytes was performed with the aim to predict the genotoxic potential of selected paraben esters, serving as a model chemical group. Parabens are widely used in consumer products as preservatives and have been reported to exhibit inconclusive results in numerous genotoxicity studies. The Comet Assay identified Ethylparaben and Benzylparaben as potentially genotoxic. The Chromosomal Aberration test revealed weak genotoxic potential in case of Ethylparaben and positive genotoxicity in case of Butylparaben, Propylparaben and Isopropylparaben. The main reasons for variability seem to be limited water solubility of parabens, determining their bioavailability at the cellular level, and absence of metabolic activation in the Comet Assay. The results confirmed that the Comet Assay should serve as a screening test and should not be used as a stand-alone method for classification of genotoxicity. The weight of evidence approach in risk assessment should be supported with data generated with the use of human relevant in vitro methods based on cells / tissues of human origin.

Effects of melatonin on N-nitroso-N-methylurea-induced carcinogenesis in rats and mutagenesis in vitro (Ames test and COMET assay)

1999 ◽  
Vol 138 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Sergei A Musatov ◽  
Vladimir N Anisimov ◽  
Véronique André ◽  
Carole Vigreux ◽  
Thierry Godard ◽  
...  
Keyword(s):  
Comet Assay ◽  
Ames Test

scholarly journals Alkaline Comet Assay using the monocytic cell line THP-1

2019 ◽  
Author(s):  
Ana Neves-Costa ◽  
Dora Pedroso ◽  
Luis F Moita
Keyword(s):  
Comet Assay ◽  
Cell Line ◽  
Adherent Cell ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Strand Breaks ◽  
Human Monocytic Cell Line ◽  
Human Monocytic Cell ◽  
Dna Strand

Abstract This protocol details the experimental procedure for performing the comet assay, a very sensitive DNA break assay based on single cell gel electrophoresis.The analysis of DNA strand breaks, both single- and double-strand breaks (SSBs and DSBs, respectively), was performed in immune responsive cells. The cell line used was the human monocytic cell line THP-1, an adherent cell type with many known applications in in vitro studies of innate immunity. The comet assay is a robust procedure that allows the accurate and reproducible quantification of DNA damage. Here we describe not only the comet assay step-by-step protocol, but also some important aspects related to troubleshooting.

scholarly journals In vitro cytotoxic and teratogenic potential of sediment extracts from Skadar Lake using fish cell line RTL-W1 and Danio rerio embryos

2013 ◽  
Vol 65 (4) ◽  
pp. 1539-1546 ◽  
Author(s):  
Andrej Perovic ◽  
Svetlana Perovic ◽  
Thomas-Benjamin Seiler ◽  
Henner Hollert
Keyword(s):  
Cell Line ◽  
Danio Rerio ◽  
Fish Cell Line ◽  
Fish Cell ◽  
Teratogenic Potential ◽  
Danio Rerio Embryos

Comparative genotoxicity testing of rhine river sediment extracts using the comet assay with permanent fish cell lines (rtg-2 and rtl-w1) and the ames test*

2004 ◽  
Vol 4 (2) ◽  
pp. 84-94 ◽  
Author(s):  
Thomas Kosmehl ◽  
Falk Krebs ◽  
Werner Manz ◽  
Lothar Erdinger ◽  
Thomas Braunbeck ◽  
...  
Keyword(s):  
Comet Assay ◽  
Cell Lines ◽  
Ames Test ◽  
River Sediment ◽  
Fish Cell ◽  
Rhine River ◽  
Fish Cell Lines ◽  
Genotoxicity Testing

scholarly journals Multiple-endpoint in vitro carcinogenicity test in human cell line TK6 distinguishes carcinogens from non-carcinogens and highlights mechanisms of action

2020 ◽  
Author(s):  
Katherine E. Chapman ◽  
Eleanor C. Wilde ◽  
Fiona M. Chapman ◽  
Jatin R. Verma ◽  
Ume-Kulsoom Shah ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cell Line ◽  
Test System ◽  
In Vitro Tests ◽  
Correct Identification ◽  
In Vitro Test ◽  
Human Lymphoblastoid Cell ◽  
Carcinogenic Potential

Abstract Current in vitro genotoxicity tests can produce misleading positive results, indicating an inability to effectively predict a compound’s subsequent carcinogenic potential in vivo. Such oversensitivity can incur unnecessary in vivo tests to further investigate positive in vitro results, supporting the need to improve in vitro tests to better inform risk assessment. It is increasingly acknowledged that more informative in vitro tests using multiple endpoints may support the correct identification of carcinogenic potential. The present study, therefore, employed a holistic, multiple-endpoint approach using low doses of selected carcinogens and non-carcinogens (0.001–770 µM) to assess whether these chemicals caused perturbations in molecular and cellular endpoints relating to the Hallmarks of Cancer. Endpoints included micronucleus induction, alterations in gene expression, cell cycle dynamics, cell morphology and bioenergetics in the human lymphoblastoid cell line TK6. Carcinogens ochratoxin A and oestradiol produced greater Integrated Signature of Carcinogenicity scores for the combined endpoints than the “misleading” in vitro positive compounds, quercetin, 2,4-dichlorophenol and quinacrine dihydrochloride and toxic non-carcinogens, caffeine, cycloheximide and phenformin HCl. This study provides compelling evidence that carcinogens can successfully be distinguished from non-carcinogens using a holistic in vitro test system. Avoidance of misleading in vitro outcomes could lead to the reduction and replacement of animals in carcinogenicity testing.

Sign in / Sign up

Export Citation Format

Share Document