Effects of melatonin on N-nitroso-N-methylurea-induced carcinogenesis in rats and mutagenesis in vitro (Ames test and COMET assay)

1999 ◽  
Vol 138 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Sergei A Musatov ◽  
Vladimir N Anisimov ◽  
Véronique André ◽  
Carole Vigreux ◽  
Thierry Godard ◽  
...  
Keyword(s):  
Comet Assay ◽  
Ames Test

scholarly journals Assessment of the genotoxic potential of Lake Skadar sediments using Ames test and comet assay on the fish cell line RTL-W1

2012 ◽  
Vol 64 (1) ◽  
pp. 249-256 ◽  
Author(s):  
A. Perovic ◽  
Svetlana Perovic ◽  
L. Erdinger ◽  
H. Hollert
Keyword(s):  
Comet Assay ◽  
Cell Line ◽  
Ames Test ◽  
In Vitro Tests ◽  
Fish Cell ◽  
Genotoxic Potential ◽  
Permanent Cell ◽  
Organic Fractions ◽  
Lake Skadar

In this study we evaluated the genotoxic potential of surface sediment extracts of Lake Skadar using a combination of two in vitro tests: the Comet assay on the fibroblast-like permanent cell line RTL-W1, and the Ames test on the strain Salmonella typhimurium TA98. The obtained results show that both tests were successful in determining the genotoxic potential in the sediment organic fractions. They possess enough sensitivity to detect early warning signals and evaluate the genotoxic potential in sediments of the Lake. The genotoxic potential was recorded and compared in the sediment samples from different locations on the Lake Skadar.

Genotoxicity evaluation in vitro by comet assay of compound and crude extract obtained from P. Pubescens Benth.

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
VHS Souza ◽  
A Paula OHohne ◽  
R Grando ◽  
N de Cassia de Almeida Queiroz ◽  
GM Pastore ◽  
...  
Keyword(s):  
Comet Assay ◽  
Crude Extract

scholarly journals Validation of the in vitro comet assay for DNA cross-links and altered bases detection

2021 ◽  
Author(s):  
Damián Muruzabal ◽  
Julen Sanz-Serrano ◽  
Sylvie Sauvaigo ◽  
Bertrand Treillard ◽  
Ann-Karin Olsen ◽  
...  
Keyword(s):  
Comet Assay ◽  
Human Health Risk ◽  
High Sensitivity ◽  
Dna Lesions ◽  
Dna Glycosylase ◽  
Vitro Assay ◽  
Cytotoxic Compound ◽  
Endonuclease Iii ◽  
Cross Links

AbstractMechanistic toxicology is gaining weight for human health risk assessment. Different mechanistic assays are available, such as the comet assay, which detects DNA damage at the level of individual cells. However, the conventional alkaline version only detects strand breaks and alkali-labile sites. We have validated two modifications of the in vitro assay to generate mechanistic information: (1) use of DNA-repair enzymes (i.e., formamidopyrimidine DNA glycosylase, endonuclease III, human 8-oxoguanine DNA glycosylase I and human alkyladenine DNA glycosylase) for detection of oxidized and alkylated bases as well as (2) a modification for detecting cross-links. Seven genotoxicants with different mechanisms of action (potassium bromate, methyl methanesulfonate, ethyl methanesulfonate, hydrogen peroxide, cisplatin, mitomycin C, and benzo[a]pyrene diol epoxide), as well as a non-genotoxic compound (dimethyl sulfoxide) and a cytotoxic compound (Triton X-100) were tested on TK-6 cells. We were able to detect with high sensitivity and clearly differentiate oxidizing, alkylating and cross-linking agents. These modifications of the comet assay significantly increase its sensitivity and its specificity towards DNA lesions, providing mechanistic information regarding the type of damage.

scholarly journals Assessment by Ames test and comet assay of toxicity potential of polymer used to develop field-capable rapid-detection device to analyze environmental samples

2014 ◽  
Vol 5 (6) ◽  
pp. 763-769 ◽  
Author(s):  
Amanda Hebert ◽  
Michelle Bishop ◽  
Dhiman Bhattacharyya ◽  
Karen Gleason ◽  
Stephen Torosian
Keyword(s):  
Comet Assay ◽  
Rapid Detection ◽  
Environmental Samples ◽  
Ames Test ◽  
Toxicity Potential

DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro

2017 ◽  
Vol 324 ◽  
pp. 781-788 ◽  
Author(s):  
Cristina Araujo Matzenbacher ◽  
Ana Letícia Hilario Garcia ◽  
Marcela Silva dos Santos ◽  
Caroline Cardoso Nicolau ◽  
Suziane Premoli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Coal Combustion ◽  
Micronucleus Test ◽  
Coal Dust ◽  
Bottom Ash

Synthesis, cytotoxicity in vitro, apoptosis, cell cycle arrest and comet assay of asymmetry ruthenium(II) complexes

Polyhedron ◽  
2016 ◽  
Vol 106 ◽  
pp. 115-124 ◽  
Author(s):  
Cheng Zhang ◽  
Chuan-Chuan Zeng ◽  
Shang-Hai Lai ◽  
De-Gang Xing ◽  
Wei Li ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Comet Assay ◽  
Cell Cycle Arrest ◽  
Cycle Arrest

scholarly journals Corrigendum to: Standardisation of the in vitro comet assay: influence of lysis time and lysis solution composition on the detection of DNA damage induced by X-rays

Mutagenesis ◽  
2019 ◽  
Vol 34 (5-6) ◽  
pp. 431-431
Author(s):  
José M Enciso ◽  
Kristine B Gutzkow ◽  
Gunnar Brunborg ◽  
Ann-Karin Olsen ◽  
Adela López de Cerain ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
X Rays ◽  
Solution Composition ◽  
Lysis Time
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