The influence of molecular weight of polyethylene glycol on separation and purification of pectinases from Penicillium cyclopium in aqueous two-phase system

In this study the possibility of the partitioning and purification of pectinases from Penicillium cyclopium by their partitioning in polymer/polymer and polymer/salt aqueous two-phase systems was investigated. In the system with 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500 000/85% (w/w) crude enzyme, the highest values for partitioning parameters were achieved - the partition coefficient was 2.11, followed by the top phase yield of 85.68% and purification factor 1.28 for the endo-pectinase activity. The partition coefficient, yield in the top phase and purification factor for the exo-pectinase activity in the same system were 1.89, 84.28% and 3.82, respectively. In the system with 10% (w/w) polyethylene glycol 6000/15% (w/w) (NH4)2SO4/75% (w/w) crude enzyme purification factor 37.85 for exo-pectinase, and 19.52 for endo-pectinase in the bottom phase were obtained.

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Extraction of Lipase from Rice Bran Using Aqueous Two-Phase System

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.651.384 ◽

2013 ◽

Vol 651 ◽

pp. 384-388 ◽

Cited By ~ 1

Author(s):

Qing Yun Wang ◽

Qin Lu Lin ◽

Xiao Hao Li

Keyword(s):

Partition Coefficient ◽

Rice Bran ◽

Crude Enzyme ◽

Phase System ◽

Two Phase ◽

Aqueous Two Phase System ◽

Activity Recovery

Aqueous two-phase system (ATPS) is an efficient and economical bioseparation technique. In this paper, the PEG2000/(NH4)2SO4 aqueous two-phase system was used to extract lipase from rice bran, and the best conditions were 24% PEG2000, 14% (NH4)2SO4, 15% addition of crude enzyme and pH 7.5. At this condition, the partition coefficient and activity recovery were highest and the activity recovery could achieve 90.29%.

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Increase of vanillin partitioning using aqueous two phase system with promising nanoparticles

Scientific Reports ◽

10.1038/s41598-019-56120-8 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Mitra Nouri ◽

Shahla Shahriari ◽

Gholamreza Pazuki

Keyword(s):

Polyethylene Glycol ◽

Partition Coefficient ◽

Molecular Mechanism ◽

Phase System ◽

Two Phase ◽

Salting Out ◽

Optimal Separation ◽

Aqueous Two Phase Systems ◽

Modified Carbon Nanotubes ◽

The Impact

AbstractThe distinct features of ATPSs (aqueous two-phase systems) have made it possible to promote the extraction efficiency of biomolecules. The purpose of this study is to discover an appropriate nanoparticle to design an economical optimal separation process, and to understand the underlying molecular mechanism which allows the partitioning of vanillin as a phenolic compound using nanoparticle-based ATPSs. To this aim, the capabilities of several different nanoparticles were investigated as additives for boosting the partition coefficient of vanillin in two different ATPSs made up of polyethylene glycol and sodium sulfate/polyethylene glycol and dextran. Also, in an attempt to explain the salting-out effect, the NRTL (Non-random Two Liquid) thermodynamic model was applied. The impact of very small amounts of modified carbon nanotubes on the enhancement of the partition coefficient of vanillin in the ATPS consisting of the biocompatible polymer(s) and salt was quite remarkable. The results showed that the partition coefficient of vanillin grew by almost 127 percent compared to the system without nanoparticle. The molecular mechanism underlying the increase in the partition coefficient was interpreted by taking advantage of structural analyses.

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Interphase distribution of 2-furylethylenes

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19851642 ◽

1985 ◽

Vol 50 (8) ◽

pp. 1642-1647 ◽

Cited By ~ 2

Author(s):

Štefan Baláž ◽

Anton Kuchár ◽

Ernest Šturdík ◽

Michal Rosenberg ◽

Ladislav Štibrányi ◽

...

Keyword(s):

Partition Coefficient ◽

Partition Coefficients ◽

Transport Rate ◽

Phase System ◽

Two Phase ◽

Interphase Distribution ◽

Distribution Kinetics ◽

System 1 ◽

Kinetics Of

The distribution kinetics of 35 2-furylethylene derivatives in two-phase system 1-octanol-water was investigated. The transport rate parameters in direction water-1-octanol (l1) and backwards (l2) are partition coefficient P = l1/l2 dependent according to equations l1 = logP - log(βP + 1) + const., l2 = -log(βP + 1) + const., const. = -5.600, β = 0.261. Importance of this finding for assesment of distribution of compounds under investigation in biosystems and also the suitability of the presented method for determination of partition coefficients are discussed.

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