Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems
Keyword(s):
A Value
◽
This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same phase.
2014 ◽
Vol 59
(2)
◽
pp. 382-390
◽
Keyword(s):
2007 ◽
Vol 859
(2)
◽
pp. 222-228
◽
2000 ◽
Vol 743
(1-2)
◽
pp. 181-185
◽
Keyword(s):
2010 ◽
Vol 88
(1)
◽
pp. 40-46
◽
Keyword(s):
1992 ◽
Vol 37
(2)
◽
pp. 268-274
◽
2014 ◽
Vol 49
(17)
◽
pp. 2741-2747
◽
Keyword(s):
1996 ◽
Vol 18
(4)
◽
pp. 251-260
◽
Keyword(s):
2008 ◽
Vol 1196-1197
◽
pp. 117-124
◽
Keyword(s):