Bioremediation of soil heavily contaminated with crude oil and its products: Composition of the microbial consortium

Bioremediation, a process that utilizes the capability of microorganism to degrade toxic waste, is emerging as a promising technology for the treatment of soil and groundwater contamination. The technology is very effective in dealing with petroleum hydrocarbon contamination. The aim of this study was to examine the composition of the microbial consortium during the ex situ experiment of bioremediation of soil heavily contaminated with crude oil and its products from the Oil Refinery Pancevo, Serbia. After a 5.5-month experiment with biostimulation and bioventilation, the concentration of the total petroleum hydrocarbons (TPH) had been reduced from 29.80 to 3.29 g/kg (89 %). In soil, the dominant microorganism population comprised Gram-positive bacteria from actinomycete-Nocardia group. The microorganisms which decompose hydrocarbons were the dominant microbial population at the end of the process, with a share of more than 80 % (range 107 CFU/g). On the basis of the results, it was concluded that a stable microbial community had been formed after initial fluctuations.

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Transformation of a petroleum pollutant during soil bioremediation experiments

Journal of the Serbian Chemical Society ◽

10.2298/jsc0805577j ◽

2008 ◽

Vol 73 (5) ◽

pp. 577-583 ◽

Cited By ~ 3

Author(s):

B. Jovancicevic ◽

M. Antic ◽

M. Vrvic ◽

M. Ilic ◽

M. Novakovic ◽

...

Keyword(s):

Microbial Consortium ◽

Polluted Soil ◽

Oil Refinery ◽

Danube River ◽

Ex Situ ◽

Insoluble Residue ◽

Soil Bioremediation ◽

Alluvial Formation ◽

Oil Type ◽

Petroleum Pollutant

The experiment of ex situ soil bioremediation was performed at the locality of the Oil Refinery in Pancevo (alluvial formation of the Danube River, Serbia) polluted with an oil type pollutant. The experiments of biostimulation, bioventilation and reinoculation of an autochthonous microbial consortium were performed during the six-month period (May-November 2006). The changes in the quantity and composition of the pollutant, or the bioremediation effect, were monitored by analysis of the samples of the polluted soil taken in time spans of two weeks. In this way, from the beginning until the end of the experiment, 12 samples were collected and marked as P1-P12 (Pancevo 1-Pancevo 12). The results obtained showed that more significant changes in the composition of the oil pollutant occurred only during the last phases of the experiment (P8-P12). The activity of microorganisms was reflected in the increase of the quantity of polar oil fractions, mainly fatty acid fractions. In this way, the quantity of total eluate increased, and the quantity of the insoluble residue was reduced to a minimum, whereby the oil pollutant was transformed to a form that could be removed more efficiently and more completely from the soil, as a segment of the environment.

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Research on Vulnerability of Aquifer to Petroleum Hydrocarbon Basing on the ANN and GIS

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.319.331 ◽

2013 ◽

Vol 319 ◽

pp. 331-336

Author(s):

Yun Zhi Fang ◽

Zhen Min Ma ◽

Yun Yun Luo

Keyword(s):

Petroleum Hydrocarbon ◽

Vulnerability Analysis ◽

Oil Refinery ◽

Hydrocarbon Contamination ◽

Ann Model ◽

Petroleum Hydrocarbon Contamination ◽

Groundwater Aquifer ◽

Factor Combination ◽

Thematic Layer ◽

Fissure Water

We set the karst fissure water where a oil refinery is located as a study object. Quantitative method of vulnerability analysis is raised for petroleum hydrocarbon contamination groundwater system in multiple factor combination conditions.We established the ANN model structure depending on the selected evaluation factors and obtained the weight of each factor coefficient, then superposed the weights of each factor thematic layer by applying ANN and GIS,and put forward model system of vulnerability analysis which petroleum hydrocarbon contaminate groundwater aquifer. Spatial distribution of antifouling performance is finally achieved of which groundwater aquifer is polluted by petroleum hydrocarbon.

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Bioremediation of actual soil samples with high levels of crude oil using a bacterial consortium isolated from two polluted sites: investigation of the survival of the bacteria

Global NEST Journal ◽

10.30955/gnj.002681 ◽

2018 ◽

Vol 20 (2) ◽

pp. 432-438 ◽

Cited By ~ 1

Keyword(s):

Crude Oil ◽

Petroleum Hydrocarbons ◽

Sole Source ◽

Bacterial Consortium ◽

Soil Samples ◽

Oil Refinery ◽

Total Petroleum Hydrocarbons ◽

Biochemical Tests ◽

Polluted Sites ◽

Achromobacter Sp

In this study, actual soil samples, taken from Kharg Island, Iran, with high levels of total petroleum hydrocarbons (TPHs) were bioremediated by a bacterial consortium. The strains were: Pseudomonas sp., Stenotrophomonas sp., Achromobacter sp., Brevibacillus sp. and Staphylococcus sp., which were isolated from two highly polluted sites: Kharg Island and Kermanshah Oil Refinery. The bacteria were identified and confirmed by biochemical tests and the PCR assay. In order to isolate the bacteria, the enrichment method, in which crude oil (2%) was utilized as the sole source of carbon and energy, was used. The results showed that the adapted bacterial consortium could degrade nearly 60% of the pollution. Also, it was found that after 100 days of bioremediation only Brevibacillus sp. could survive in the environment.

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Ground-water contamination by crude oil at the Bemidji, Minnesota, research site- An introduction: Chapter A in Ground-water contamination by crude oil at the Bemidji, Minnesota, research site; US Geological Survey Toxic Waste--ground-water contamination study

10.3133/wri844188a ◽

1984 ◽

Keyword(s):

Crude Oil ◽

Ground Water ◽

Water Contamination ◽

Toxic Waste ◽

Geological Survey ◽

Ground Water Contamination ◽

Research Site

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An accelerated solvent extraction and gas chromatography-flame ionization detector method to rapidly determining and assessing total petroleum hydrocarbon contamination in soil from Fushan oilfield, China

Environmental Science and Pollution Research ◽

10.1007/s11356-020-09418-4 ◽

2020 ◽

Vol 27 (30) ◽

pp. 37444-37454

Author(s):

Tengya Li ◽

Xiaocong Cao ◽

Shuhai He ◽

Mingshan Zhang ◽

Wenshuai Xu ◽

...

Keyword(s):

Gas Chromatography ◽

Solvent Extraction ◽

Petroleum Hydrocarbon ◽

Total Petroleum Hydrocarbon ◽

Flame Ionization Detector ◽

Hydrocarbon Contamination ◽

Ionization Detector ◽

Flame Ionization ◽

Petroleum Hydrocarbon Contamination ◽

Detector Method

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Efficiency and Kinetics of Total Petroleum Hydrocarbons (TPHs) Removal from Crude Oil Polluted Arable Soil using Palm Bunch Ash and Tween 80

Chemistry Africa ◽

10.1007/s42250-020-00219-3 ◽

2021 ◽

Author(s):

Godwin James Udo ◽

Nnanake-Abasi O. Offiong ◽

Alfreda Nwadinigwe ◽

Clement O. Obadimu ◽

Aniedi E. Nyong ◽

...

Keyword(s):

Crude Oil ◽

Petroleum Hydrocarbons ◽

Tween 80 ◽

Arable Soil ◽

Total Petroleum Hydrocarbons ◽

Kinetics Of ◽

Palm Bunch

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Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

Environmental Science and Pollution Research ◽

10.1007/s11356-021-13885-8 ◽

2021 ◽

Author(s):

Emilio D’Ugo ◽

Milena Bruno ◽

Arghya Mukherjee ◽

Dhrubajyoti Chattopadhyay ◽

Roberto Giuseppetti ◽

...

Keyword(s):

Oil Spill ◽

Petroleum Hydrocarbon ◽

Oil Spills ◽

Oil Pollution ◽

Primary Source ◽

Hydrocarbon Contamination ◽

Petroleum Hydrocarbon Contamination ◽

Microbial Response ◽

Pollution Event ◽

Hydrocarbonoclastic Potential

AbstractMicrobiomes of freshwater basins intended for human use remain poorly studied, with very little known about the microbial response to in situ oil spills. Lake Pertusillo is an artificial freshwater reservoir in Basilicata, Italy, and serves as the primary source of drinking water for more than one and a half million people in the region. Notably, it is located in close proximity to one of the largest oil extraction plants in Europe. The lake suffered a major oil spill in 2017, where approximately 400 tons of crude oil spilled into the lake; importantly, the pollution event provided a rare opportunity to study how the lacustrine microbiome responds to petroleum hydrocarbon contamination. Water samples were collected from Lake Pertusillo 10 months prior to and 3 months after the accident. The presence of hydrocarbons was verified and the taxonomic and functional aspects of the lake microbiome were assessed. The analysis revealed specialized successional patterns of lake microbial communities that were potentially capable of degrading complex, recalcitrant hydrocarbons, including aromatic, chloroaromatic, nitroaromatic, and sulfur containing aromatic hydrocarbons. Our findings indicated that changes in the freshwater microbial community were associated with the oil pollution event, where microbial patterns identified in the lacustrine microbiome 3 months after the oil spill were representative of its hydrocarbonoclastic potential and may serve as effective proxies for lacustrine oil pollution.

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