scholarly journals Diagnostic value of certain methods for isolation of Mycobacterium tuberculosis in children with suspected pulmonary tuberculosis

2005 ◽  
Vol 58 (5-6) ◽  
pp. 231-235 ◽  
Author(s):  
Slobodanka Petrovic
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Gastric Lavage ◽  
Lavage Fluid ◽  
Diagnostic Value ◽  
Bacteriological Diagnosis ◽  
Tuberculosis In Children ◽  
Better Than

Introduction The aim of this study was to find out if bronchoalveolar lavage (BL) is better than gastric lavage (GL) or sputum (SP) examination for isolation of Mycobacterium tuberculosis in childhood pulmonary tuberculosis. Material and methods The study included a group of 30 children with suspected pulmonary tuberculosis, aged 6 months to 18 years. Gastric lavage was done on 4 consecutive mornings after overnight fast. Sputum was examined in 12 patients older than 10 years, (4 consecutive samples). BL was performed on the same day as the last GL and SP, using a rigid bronchoscope. Specimens were examined for the existence of BL acid fast bacilli (AFB) and culture for Mycobacterium tb. Results Out of 30 cases, Mycobacterium tb was grown in 13 BL samples (43.33%), and in 10 GL samples from the same patients (33.33%). Comparative analysis of microbiological samples of sputum and bronchoalveolar lavage fluid in 12 children showed that 6 patients had SP+ cultures and 6 patients had SP-cultures of Mycobacterium tb. All SP+ patients where also BL+, but 1 SP- patient was BL+. Smear examination of BL fluid samples was positive for AFB in 10 patients with positive cultures for Mycobacterium tb. Conclusion The results of our study indicate that BL examination is better than GL and SP examination for bacteriological diagnosis of childhood tuberculosis. .

scholarly journals The value of GeneXpert MTB/RIF in bronchoalveolar lavage fluid in the diagnosis of smear-negative pulmonary tuberculosis

2021 ◽  
Vol 62 (1) ◽  
pp. 28-36
Author(s):  
Yue-Ying Zhou ◽  
Ji-Chan Shi ◽  
Ning Pan ◽  
Zheng-Xing Wu ◽  
Ai-Qiong Cheng ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Bronchoalveolar Lavage ◽  
Liquid Culture ◽  
Drug Sensitivity ◽  
Bronchoalveolar Lavage Fluid ◽  
Culture Method ◽  
Lavage Fluid ◽  
Diagnostic Value ◽  
Smear Negative ◽  
Acid Fast Staining

This study aims to evaluate the diagnostic value of Xpert MTB/ RIF assay in bronchoalveolar lavage fluid (BALF) in subjects with smear-negative pulmonary tuberculosis. From January 2019 to December 2019, 197 patients with suspected pulmonary tuberculosis were recruited, and bronchoalveolar lavage fluid was collected for acid-fast staining smear, liquid culture of Mycobacterium combined drug sensitivity and Xpert MTB/RIF detection. The sensitivity, specificity, positive predictive value and negative predictive value of Xpert MTB/RIF in bronchoalveolar lavage fluid (BALF) were calculated with smear-negative pulmonary tuberculosis as the reference standard. The consistency of xpert MTB/RIF in the diagnosis of rifampicin resistance was evaluated, with the results of Mycobacterium liquid culture drug sensitivity test and drug sensitivity test as the gold standards. The results showed that among 197 suspected tuberculosis patients, 55 patients were not diagnosed with tuberculosis and 142 patients were diagnosed with smear-negative pulmonary tuberculosis. One hundred and twenty three cases (86.62%) were positive for Xpert MTB/ RIF in bronchoalveolar lavage fluid, 15 cases (10.56%) were positive by acid-fast staining smear method, and 88 cases (61.97%) were positive by the liquid culture method. The positive rate of Xpert MTB / RIF was 93.18% (82 / 88), which was higher than that of 75.93% (41 / 54) of the negative BALF mycobacterium culture (χ 2 = 8.598, P<0.01). The sensitivity and specificity of Xpert MTB/RIF for rifampicin resistance were 100.00% and 97.30%, respectively. Therefore, the diagnostic value of Xpert MTB/RIF in bronchoalveolar lavage fluid for bacterialnegative pulmonary tuberculosis is superior to the acid-fast staining smear of lavage fluid and the mycobacterium culture method.

scholarly journals Diagnosis of Pulmonary Tuberculosis in Children

2017 ◽  
Vol 15 (11-12) ◽  
pp. 286
Author(s):  
Gunadi Santosa
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Clinical Diagnosis ◽  
Gastric Lavage ◽  
Tuberculin Test ◽  
Medical Doctor ◽  
Definite Diagnosis ◽  
Bacteriological Diagnosis ◽  
The Moment ◽  
Tuberculosis In Children

1. A definite diagnosis of tuberculosis can only be established on the basis of the finding of the tubercle bacilli.2. The isolation of the mycobacterium tuberculosis from a child is subject to difficulties:a. the necessity of gastric lavage for 3 consecutive days, which is difficult to perform ambulatory.b. the necessity of the proper facilities of a microbiologic laboratory.c. the outcome is often not sufficiently high.3. It is concluded that at the moment making of the bacteriological diagnosis of child tuberculosis is not practical, so that we are forced to depend on the clinical diagnosis.4. The tuberculin test is very important in establishing the diagnosis. It is recommended to perform this test routinely on every child once a year and also that every medical doctor as well as specialist performs this test to detect a case of TBC at the earliest possible moment.

scholarly journals Diagnostic value of nucleic acid amplification tests on bronchoalveolar lavage fluid for smear-negative pulmonary tuberculosis: a meta-analysis

2015 ◽  
Vol 35 (4) ◽  
Author(s):  
Panwen Tian ◽  
Yongchun Shen ◽  
Ye Wang ◽  
Chun Wan ◽  
Mei Feng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Pulmonary Tuberculosis ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Meta Analysis ◽  
Lavage Fluid ◽  
Diagnostic Value ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Tests ◽  
Smear Negative

Based on current available evidence, NAAT on BALF plays a role in the diagnosis of smear-negative pulmonary tuberculosis, and further studies should be performed to confirm our findings..

Sign in / Sign up

Export Citation Format

Share Document