scholarly journals Diagnosis of Pulmonary Tuberculosis in Children

2017 ◽  
Vol 15 (11-12) ◽  
pp. 286
Author(s):  
Gunadi Santosa
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Clinical Diagnosis ◽  
Gastric Lavage ◽  
Tuberculin Test ◽  
Medical Doctor ◽  
Definite Diagnosis ◽  
Bacteriological Diagnosis ◽  
The Moment ◽  
Tuberculosis In Children

1. A definite diagnosis of tuberculosis can only be established on the basis of the finding of the tubercle bacilli.2. The isolation of the mycobacterium tuberculosis from a child is subject to difficulties:a. the necessity of gastric lavage for 3 consecutive days, which is difficult to perform ambulatory.b. the necessity of the proper facilities of a microbiologic laboratory.c. the outcome is often not sufficiently high.3. It is concluded that at the moment making of the bacteriological diagnosis of child tuberculosis is not practical, so that we are forced to depend on the clinical diagnosis.4. The tuberculin test is very important in establishing the diagnosis. It is recommended to perform this test routinely on every child once a year and also that every medical doctor as well as specialist performs this test to detect a case of TBC at the earliest possible moment.

scholarly journals Diagnostic value of certain methods for isolation of Mycobacterium tuberculosis in children with suspected pulmonary tuberculosis

2005 ◽  
Vol 58 (5-6) ◽  
pp. 231-235 ◽  
Author(s):  
Slobodanka Petrovic
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Gastric Lavage ◽  
Lavage Fluid ◽  
Diagnostic Value ◽  
Bacteriological Diagnosis ◽  
Tuberculosis In Children ◽  
Better Than

Introduction The aim of this study was to find out if bronchoalveolar lavage (BL) is better than gastric lavage (GL) or sputum (SP) examination for isolation of Mycobacterium tuberculosis in childhood pulmonary tuberculosis. Material and methods The study included a group of 30 children with suspected pulmonary tuberculosis, aged 6 months to 18 years. Gastric lavage was done on 4 consecutive mornings after overnight fast. Sputum was examined in 12 patients older than 10 years, (4 consecutive samples). BL was performed on the same day as the last GL and SP, using a rigid bronchoscope. Specimens were examined for the existence of BL acid fast bacilli (AFB) and culture for Mycobacterium tb. Results Out of 30 cases, Mycobacterium tb was grown in 13 BL samples (43.33%), and in 10 GL samples from the same patients (33.33%). Comparative analysis of microbiological samples of sputum and bronchoalveolar lavage fluid in 12 children showed that 6 patients had SP+ cultures and 6 patients had SP-cultures of Mycobacterium tb. All SP+ patients where also BL+, but 1 SP- patient was BL+. Smear examination of BL fluid samples was positive for AFB in 10 patients with positive cultures for Mycobacterium tb. Conclusion The results of our study indicate that BL examination is better than GL and SP examination for bacteriological diagnosis of childhood tuberculosis. .

scholarly journals “Induced sputum versus gastric lavage for the diagnosis of pulmonary tuberculosis in children”

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Marta Ruiz Jiménez ◽  
Sara Guillén Martín ◽  
Luis M Prieto Tato ◽  
Juana B Cacho Calvo ◽  
Ana Álvarez García ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Gastric Lavage ◽  
Induced Sputum ◽  
Tuberculosis In Children

Detection of Mycobacterium tuberculosis in Clinical Specimens From Children Using a Polymerase Chain Reaction

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 155-160 ◽  
Author(s):  
Kim Connelly Smith ◽  
Jeffrey R. Starke ◽  
Kathleen Eisenach ◽  
Lydia T. Ong ◽  
Melissa Denby
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Clinical Diagnosis ◽  
Tuberculosis Infection ◽  
Chain Reaction ◽  
Clinical Specimens ◽  
Mycobacterial Culture ◽  
Polymerase Chain ◽  
Tuberculosis In Children ◽  
Culture Negative

Objective. We evaluated the usefulness of the polymerase chain reaction (PCR) using the insertion sequence IS6110 as the target for DNA to detect Mycobacterium tuberculosis in clinical specimens from children. Study Design. This was a prospective, controlled, blinded study comparing PCR on clinical specimens, mycobacterial culture, and clinical diagnosis. Patients. Sixty-five hospitalized children were evaluated, 35 with tuberculosis disease and 30 controls. Cases were defined by culture and/or specific clinical criteria. Controls included patients with tuberculosis infection but no detectable disease as well as patients free of tuberculosis infection and disease. Results. Polymerase chain reaction had a sensitivity of 40% and a specificity of 80% compared with clinical diagnosis. Mycobacterial culture had a sensitivity of 37%. The combination of culture and PCR identified 19 of 35 children (54%) with clinically diagnosed tuberculosis. There were six children with false-positive PCR results: One had tuberculosis infection without disease, two had Mycobacterium avium lymphadenitis, and three had diagnoses unrelated to tuberculosis. Conclusions. The sensitivity of PCR is comparable to that of culture for detecting M tuberculosis in children, and may strengthen and hasten the clinical diagnosis in culture-negative patients. However, because of the limitations in specificity, the results of PCR alone are insufficient to diagnose tuberculosis in children. Although ongoing refinements in PCR techniques should improve the specificity of this test, epidemiologic and clinical information continue to be the most important consideration in the diagnosis of tuberculosis in culture-negative children.

Usefulness of GeneXpert MTB/RIF in the diagnosis of extra-pulmonary tuberculosis

2021 ◽  
Vol 11 (2) ◽  
pp. 121-124
Author(s):  
Nasreen Islam ◽  
Mohammad Delwar Hossain ◽  
Muhammad Abdur Rahim ◽  
Jamal Uddin Ahmed ◽  
Muhammad Kamrul Amin ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Pleural Fluid ◽  
Past History ◽  
Gastric Lavage ◽  
Extra Pulmonary Tuberculosis ◽  
Pulmonary Tb ◽  
Male Predominance ◽  
Bacteriological Diagnosis ◽  
Extra Pulmonary ◽  
Tracheal Aspirates

Background: Diagnosis of extra-pulmonary tuberculosis (TB) is often delayed because of diverse clinical presentations and difficulties in establishing the bacteriological diagnosis. This study aimed to evaluate usefulness of GeneXpert MTB/RIF in the diagnosis of extra-pulmonary TB in Bangladeshi patients. Methods: This cross-sectional study was done in BIRDEM General Hospital, Dhaka, Bangladesh from 2013 to 2016 as a part of Bangladesh Diabetic Somiti (BADAS)-USAID-TB Care-II project. Representative samples from 590 clinically suspected extra-pulmonary TB cases were tested for GeneXpert MTB/RIF along with conventional methods. Results: Total patients were 590 [mean age 43.9 (range 1-95) years] with male predominance (326, 55.3%). Most (513, 86.9%) patients were diabetic and new (574, 97.3%) TB suspects; while 16 (2.7%) patients had past history of TB. Common samples were pleural fluid (125, 21.2%), urine (110, 18.6%), cerebrospinal fluid (CSF) (91, 15.4%), pus (82, 13.9%), tracheal aspirates (57, 9.7%), ascitic fluid (45, 7.6%), gastric lavage (31, 5.3%), broncho-alveolar lavage (BAL) (18, 3.1%), lymph node aspirates (11, 1.9%) and synovial fluid (8, 1.4%). Among 590 samples, 68 (11.5%) were positive for Mycobacterium tuberculosis. Diagnostic yield was common for lymph nodes (4/7, 57.1%), pus (25/82, 30.5%), BAL (4/18, 22.2%), tracheal aspirates (8/57, 14.0%), urine (7/110, 6.4%), CSF (6/91, 6.6%) and pleural fluid (7/125, 5.6%). Of the 68 GeneXpert MTB/RIF positive samples, 52 (76.1%) were rifampicin sensitive, 16 (23.9%) showed intermediate sensitivity and none of the samples was resistant to rifampicin. Conclusions: GeneXpert MTB/RIF appeared as useful tool for diagnosing extra-pulmonary TB. Birdem Med J 2021; 11(2): 121-124

Value of bronchoalveolar lavage and gastric lavage in the diagnosis of pulmonary tuberculosis in children

1995 ◽  
Vol 76 (4) ◽  
pp. 295-299 ◽  
Author(s):  
N. Somu ◽  
S. Swaminathan ◽  
C.N. Paramasivan ◽  
D. Vijayasekaran ◽  
A. Chandrabhooshanam ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Bronchoalveolar Lavage ◽  
Gastric Lavage ◽  
Tuberculosis In Children

scholarly journals Saline Nebulization before Gastric Lavage in the Diagnosis of Pulmonary Tuberculosis in Children and Adolescents

2010 ◽  
Vol 56 (6) ◽  
pp. 458-459 ◽  
Author(s):  
E. L. Maciel ◽  
R. Lyrio Peres ◽  
T. N. do Prado ◽  
C. R. Macedo ◽  
M. Palaci ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Children And Adolescents ◽  
Gastric Lavage ◽  
Tuberculosis In Children
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