scholarly journals Seroprevalence of dogs to Leishmania infantum antigens in the territory of Crna Gora

2014 ◽  
Vol 68 (5-6) ◽  
pp. 301-309
Author(s):  
Zorica Pavicevic ◽  
Dejan Lausevic ◽  
Nikola Pejovic
Keyword(s):  
Leishmania Infantum ◽  
Clinical Symptoms ◽  
Fluorescent Antibody ◽  
Good Condition ◽  
Hypochromic Anemia ◽  
World Health ◽  
Fluorescent Antibody Technique ◽  
Serum Samples ◽  
Antibody Technique ◽  
Mediterranean Countries

Due to expansion of vector borne diseases and their importance for human health we have investigated the prevalence of antibodies to Leishmania infantum antigens in serum samples of dogs. Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania. According to the World Health Organization the disease is a constant threat to 350 million people in 88 countries, provided that most Mediterranean countries belong to the area designated as endemic. Since Crna Gora is a country of Mediterranean basin, it is important to present the results of the investigation on the presence of antibodies on L. infantum antigens in blood serum of dogs, considering that dogs can be reservoirs and source of infection for humans. In the period from 2006. to 2010., there were tested 433 serum samples of dogs with clinical symptoms and 68 serum samples of dogs without symptoms of the disease. Those were dogs with visible symptoms of the disease such as alopecia, weight loss and lymphadenomegalia. In 66% of cases, there was known hematological profile with predominant hypochromic anemia and neutrophilic leukocytosis. As mentioned above, the investigation included also 68 asymptomatic dogs in good condition, out of which 45 were service dogs in use in military and police as well as 23 dogs from the asylum. By Indirect immunofluorescence test or Indirect Fluorescent Antibody Technique (IF-AT) and Direct Agglutination Test (DAT), there was determined the presence of specific antibodies to L. infantum antigens. Positive findings in the serum dilution 1:160 was deter?mined in 258 samples (59.58%) in the group of dogs with clinical symptoms, and in 5.9% in the tested asymptomatic dogs. A part of the territory of Crna Gora with its average annual air temperature of 14.6 - 17.8?C and mediterranean climate (long, warm and dry summer and mild, rainy winter) has all the prerequisites for the development and maintenance of the infection. The confirmed presence of five kinds of Phlebotomines on the territory of Crna Gora which are essential in the cycle of developing and maintaining of Leishmania species with dominant Phlebotomus neglectus species, additionally proves a great risk of occur?rence and maintenance of the disease. The obtained results of seroprevalence in dogs are in correlation with the results of 55 cases of visceral leishmaniasis recorded by the Institute for Public Health in people from 1992 to 2009. Taking into consideration that seronegative findings and absence of symptoms does not exclude the infection, as well as that seropositive finding does not necessarily confirm it, a high level of seroprevalence (of 59.58%) in dogs in urban regions of Crna Gora, recorded during 5 years period, is by itself an alarming epizootiological and epidemiological fact.

scholarly journals Design and Construction of an Apparatus for the Growth of Micro Cell Cultures on Standard Glass Microscope Slides and Its Application for Screening Large Numbers of Sera by the Indirect Fluorescent Antibody Technique

1975 ◽  
Vol 2 (3) ◽  
pp. 153-156
Author(s):  
Michael J. P. Lawman ◽  
Ian S. Caie
Keyword(s):  
Cell Cultures ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Serum Samples ◽  
Antibody Technique ◽  
Standard Glass ◽  
Large Numbers ◽  
Microscope Slides ◽  
Glass Microscope

A simple stainless-steel apparatus was designed to contain standard microscope slides on which were grown micro cell cultures in the form of 16 individual monolayers per slide. The application of this apparatus for the screening of serum samples by fluorescent antibody techniques is described.

scholarly journals T77. ASSOCIATION BETWEEN INTRACELLULAR INFECTIOUS AGENTS AND SCHIZOPHRENIA

2020 ◽  
Vol 46 (Supplement_1) ◽  
pp. S261-S261
Author(s):  
Young-Joon Kwon ◽  
Bo-Hyun Yoon ◽  
Kwanghun Lee ◽  
Moon-Doo Kim ◽  
Beomwoo Nam ◽  
...  
Keyword(s):  
Schizophrenia Patient ◽  
Clinical Symptoms ◽  
Fluorescent Antibody ◽  
Chlamydia Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescent Antibody Technique ◽  
Igg Antibody ◽  
Antibody Technique ◽  
General Psychopathology ◽  
Control Subjects

Abstract Background A number of studies have reported association between Toxoplasma gondii (T. gondii) and Chlamydia infection and the risk of schizophrenia. The aim of the present study was to compare the prevalence of T. gondii and Chlamydia infection between the schizophrenia and normal control subjects and to compare the clinical features between seropositive and seronegative schizophrenia patients. Methods The rate of serum reactivity to T. gondii, Chlamydia trachomatis (C. trachomatis), Chlamydia pneumonia in 96 schizophrenia and 50 control subjects was investigated using enzyme-linked immunosorbent assay and indirect fluorescent antibody technique. The clinical symptoms of the schizophrenia patients were scored with Positive and Negative Syndrome Scale and a comparative analysis was carried out. Results A significant positive association between immunoglobulin G (IgG) antibodies to T. gondii and C. trachomatis in schizophrenia was found, and the odds ratio of schizophrenia associated with IgG antibody was found to be 3.22 and 2.86, respectively. The Toxoplasma-seropositive schizophrenia patient had higher score on the negative subscale N1 and N7 and general psychopathology subscale G13, while C. trachomatis-seropositive schizophrenia patient had higher score on the general psychopathology subscale G10. Discussion The results from the present study suggest significant association between T. gondii, C. trachomatis infection and schizophrenia. In future, further studies are needed to elucidate the correlation between the two types of infection and schizophrenia.

scholarly journals Identification of pregnancies at risk from cytomegalovirus infection

1977 ◽  
Vol 79 (3) ◽  
pp. 347-354 ◽  
Author(s):  
L. M. de Silva ◽  
G. L. Kampfner ◽  
C. M. Lister ◽  
J. O.'H. Tobin
Keyword(s):  
At Risk ◽  
Pregnant Women ◽  
Infection Rate ◽  
Cytomegalovirus Infection ◽  
Fluorescent Antibody ◽  
Congenital Infection ◽  
Fluorescent Antibody Technique ◽  
Serum Samples ◽  
Antibody Technique ◽  
Virus Excretion

SUMMARYThe fluorescent antibody technique was used for the identification of specific cytomegalovirus IgM in the sera of twenty-four of 1065 unmarried pregnant women. Seventeen of them were followed to term and five infected infants were identified. Two other infants had CMV IgM in neonatal serum samples but virus excretion was not demonstrated. The congenital infection rate in this study was 5·3 per 1000 births by virus excretion and 7·9 per 1000 if cases with specific IgM are included; from previous studies a rate of 8·8 per 1000 was expected. The reasons for the lack of relationship between specific IgM in the mothers' serum and infected babies is discussed.

scholarly journals Exploring possibilities of diagnosing, control and eradication of porcine respiratory and reproductive syndrome (PRRS)

2007 ◽  
Vol 23 (5-6-1) ◽  
pp. 391-401 ◽  
Author(s):  
S. Lazic ◽  
T. Petrovic
Keyword(s):  
Viral Genome ◽  
Fluorescent Antibody ◽  
Economic Losses ◽  
Fluorescent Antibody Technique ◽  
Serum Samples ◽  
Isolation And Identification ◽  
Antibody Technique ◽  
Specific Antibodies ◽  
Virus Identification ◽  
Porcine Respiratory

Porcine reproductive and respiratory syndrome is a pervasive disease that often seriously affects the health status of the animals and may lead to severe economic losses. Substantial efforts have been made to promptly diagnose this disease, and this paper presents an overview of clinical and laboratory investigation of PRRS in all animal categories within an industrial pig herd. Suspect disease was identified by clinical observation, and virus identification and detection of specific antibodies in suspect material were confirmed by laboratory examination. Isolation and identification of the virus from samples obtained from dead and sacrificed piglets was carried out on cell cultures (MARK-145) using fluorescence antibody technique, and molecular-biology techniques (RT-PCR and nested PCR) were applied for detecting viral genome in native sperm samples. Presence of PRRS-specific antibodies was investigated in blood serum samples of boars by the use of ELISA. The virus was isolated from the suspect material of sacrificed piglet, and in two more sacrificed animals the virus was detected by the fluorescent antibody technique. The PRRS viral genome could not be detected in the native sperm of 10 boars even after triple sampling. Specific antibodies against PRRS were detected in 37 out of 42 examined boars.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

1965 ◽  
Vol 13 (3) ◽  
pp. 155-160 ◽  
Author(s):  
H. J. KEUTEL
Keyword(s):  
Kidney Stones ◽  
Kidney Stone ◽  
Fluorescent Antibody ◽  
Human Kidney ◽  
Fluorescent Antibody Technique ◽  
Renal Tubules ◽  
Antibody Technique ◽  
The Matrix ◽  
Normal Human ◽  
The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

Sign in / Sign up

Export Citation Format

Share Document