scholarly journals Antibiotic resistance of hospital strains of Enterococcus faecalis and Enterococcus faecium

2002 ◽  
Vol 59 (5) ◽  
pp. 499-506 ◽  
Author(s):  
Veljko Mirovic
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Clinical Laboratory ◽  
Streptomycin Resistance ◽  
National Committee ◽  
High Incidence ◽  
Gentamicin Resistance ◽  
Vancomycin Resistant ◽  
High Level ◽  
Very High

The aim of this study was to determine the resistance of Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium) to penicillin, ampicillin, vancomycin, teicoplanin, gentamicin (high level), streptomycin (high level), oxytetracycline, chloramphenicol, rifampin, erythromycin, ciprofloxacin, norfloxacin, and nitrofurantoin from clinical specimens during 1999. The resistance of enterococci to antibiotics was determined by disk diffusion and dilution methods according to the American National Committee for Clinical Laboratory Standards guidelines. The production of ?-lactamase was determined by nitrocefin disks. In E. faecalis and E. faecium isolates (n=111 and n=48) the frequency of the resistance to both penicillins was 0.9% and 89.6%, respectively. All enterococci isolates were ?-lactamase negative. Only one strain of E. faecium was vancomycin resistant (Van A fenotype). Among E. faecalis isolates (n=109) high level gentamicin resistance (HLGR), high level streptomycin resistance (HLSR), and resistance to both agents was 52.3%, 50.4%, and 43.7%, respectively. Among E. faecium isolates (n=48) HLGR, HLSR, and to both agents were 68.7%, 75%, and 62.5% respectively. The majority of E. faecium isolates were resistant to both penicillin and ampicillin. E. faecalis remained susceptible to penicillins. Moreover, there was a very high incidence of enterococci resistant to high level aminoglycosides.

scholarly journals Epidemiologic Evaluation of Antimicrobial Resistance in Community-Acquired Enterococci

1998 ◽  
Vol 36 (3) ◽  
pp. 830-832 ◽  
Author(s):  
J. Silverman ◽  
L. A. Thal ◽  
M. B. Perri ◽  
G. Bostic ◽  
M. J. Zervos
Keyword(s):  
Risk Factors ◽  
Antimicrobial Resistance ◽  
Enterococcus Faecalis ◽  
Community Hospital ◽  
Antibiotic Use ◽  
Ampicillin Resistance ◽  
Vancomycin Resistant Enterococci ◽  
Gentamicin Resistance ◽  
Vancomycin Resistant ◽  
High Level

Fecal samples from 200 consecutive patients admitted to a community hospital yielded 107 enterococci. High-level gentamicin resistance occurred in 10 (14%) of the Enterococcus faecalisisolates. Ampicillin resistance occurred in two (3%) of the E. faecalis isolates and six (23%) of the Enterococcus faecium isolates. There were no vancomycin-resistant enterococci. Risk factors for enterococci with high-level aminoglycoside (gentamicin) or ampicillin resistance included prior hospitalization and previous antibiotic use.

scholarly journals High level of vancomycin resistance in invasive strains of Enterococcus faecalis and Enterococcus faecium in Italy: initial results from the national AR-ISS antibiotic resistance project

2002 ◽  
Vol 6 (24) ◽  
Author(s):  
D Boccia ◽  
F D'Ancona ◽  
A Pantosti
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Vancomycin Resistance ◽  
Blood Cultures ◽  
Vancomycin Resistant ◽  
High Level ◽  
Initial Results

A total of 5.9% of isolates from blood cultures of enterococci in Italy during the last half of 2001 were resistant to three or more antibiotics (of different classes) (1). The frequency of vancomycin resistance and overall multiresistance in invasive strains of Enterococcus faecalis and Enterococcus faecium are reported in table 1. It is of note that 80.0% of those isolates that were vancomycin resistant were also multiresistant.

scholarly journals Structures, Locations, and Transfer Frequencies of Genetic Elements Conferring High-Level Gentamicin Resistance in Enterococcus faecalis Isolates in Greece

2003 ◽  
Vol 47 (12) ◽  
pp. 3950-3953 ◽  
Author(s):  
George L. Daikos ◽  
George Bamias ◽  
Christos Kattamis ◽  
Marcus J. Zervos ◽  
Joseph W. Chow ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Restriction Enzyme ◽  
Plasmid Dna ◽  
Strong Association ◽  
Hybridization Analysis ◽  
Clinical Isolates ◽  
Genetic Elements ◽  
Transfer Characteristics ◽  
Gentamicin Resistance ◽  
High Level

ABSTRACT The elements conferring high-level gentamicin resistance in 64 clinical isolates of Enterococcus faecalis were characterized by PCR and by restriction enzyme hybridization analysis of genomic and plasmid DNA. There was a strong association between gentamicin resistance and the aac(6′)-aph(2") gene carried on IS256-based elements with different structures, locations, and transfer characteristics.

scholarly journals The frequency of resistance to antibiotics of most frequently isolated bacteria from blood cultures during the period 1997-2002

2004 ◽  
Vol 61 (4) ◽  
pp. 391-397
Author(s):  
Veljko Mirovic ◽  
Branka Tomanovic ◽  
Sonja Konstantinovic
Keyword(s):  
Clinical Laboratory ◽  
Blood Cultures ◽  
Diffusion Method ◽  
National Committee ◽  
Disk Diffusion Method ◽  
Beta Lactamases ◽  
Resistance To Antibiotics ◽  
Extended Spectrum Beta Lactamases ◽  
High Level

The aim of this study was to determine the frequency of resistance to antibiotics of the most frequently isolated bacteria from blood cultures of hospitalized patients during the period 1997-2002. The resistance to antibiotics was determined by disk diffusion method according to National Committee for Clinical Laboratory Standards procedures. The majority of staphylococci isolates were resistant to methicillin, and the proportion of methicillin-resistant Staphylococcus aureus was stable (76.8-81.6%), during the follow-up period. None of the staphylococci isolates were resistant to vancomycin, but there was a very high incidence of high-level resistance of enterococci to aminoglycosides (47.2-72.2%). In 1998, only one strain among enterococci was resistant to vancomycin (Enterococcus faecium, VanA fenotype). Enterococcus spp isolates expressed variable frequency of resistance to ampicillin (15-40.1%) during the follow-up period. Among Enterobacteriaceae there were no isolates resistant to imipenem, but dramatic increase of the resistance to ceftriaxone was found from 35.9% in 1997 to 95.9% in 2002 (p<0.001). Extended spectrum beta-lactamases production was found in all the species of enterobacteria isolates. Resistance to imipenem was observed in Acinetobacter spp isolates in 2002 for the first time. Pseudomonas spp isolates expressed high and very variable resistance to all antibiotics tested during the follow-up period.

Sign in / Sign up

Export Citation Format

Share Document