Aegilops crassa: Rhodes, L., Bradley, I. & Maxted, N.

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Author(s):  
Ghazal Ghobadi ◽  
Alireza Etminan ◽  
Ali Mehras Mehrabi ◽  
Lia Shooshtari

Abstract Background Evaluation of genetic diversity and relationships among crop wild relatives is an important task in crop improvement. The main objective of the current study was to estimate molecular variability within the set of 91 samples from Triticum aestivum, Aegilops cylindrica, and Aegilops crassa species using 30 CAAT box–derived polymorphism (CBDP) and start codon targeted (SCoT) markers. Results Fifteen SCoT and Fifteen CBDP primers produced 262 and 298 fragments which all of them were polymorphic, respectively. The number of polymorphic bands (NPB), polymorphic information content (PIC), resolving power (Rp), and marker index (MI) for SCoT primers ranged from 14 to 23, 0.31 to 0.39, 2.55 to 7.49, and 7.56 to 14.46 with an average of 17.47, 0.34, 10.44, and 5.69, respectively, whereas these values for CBDP primers were 15 to 26, 0.28 to 0.36, 3.82 to 6.94, and 4.74 to 7.96 with a mean of 19.87, 0.31, 5.35, and 6.24, respectively. Based on both marker systems, analysis of molecular variance (AMOVA) indicated that the portion of genetic diversity within species was more than among them. In both analyses, the highest values of the number of observed (Na) and effective alleles (Ne), Nei’s gene diversity (He), and Shannon’s information index (I) were estimated for Ae. cylindrica species. Conclusion The results of cluster analysis and population structure showed that SCoT and CBDP markers grouped all samples based on their genomic constitutions. In conclusion, the used markers are very effective techniques for the evaluation of the genetic diversity in wild relatives of wheat.



Euphytica ◽  
1993 ◽  
Vol 67 (1-2) ◽  
pp. 41-48 ◽  
Author(s):  
Koji Murai ◽  
Koichiro Tsunewaki


2011 ◽  
Vol 11 (1) ◽  
pp. 2 ◽  
Author(s):  
Kota Mizumoto ◽  
Hitoshi Hatano ◽  
Chizuru Hirabayashi ◽  
Koji Murai ◽  
Shigeo Takumi


2013 ◽  
Vol 50 ◽  
pp. 23-29
Author(s):  
Mohammad Reza Naghavi ◽  
Sayvan Ahmadi ◽  
Ali-Akbar Shanejat-Boushehri ◽  
Golnaz Komaei ◽  
Paul C. Struik


2007 ◽  
Vol 10 (7) ◽  
pp. 1126-1129 ◽  
Author(s):  
M. Ranjbar ◽  
M.R. Naghavi ◽  
A. Zali ◽  
M.J. Aghaei


2010 ◽  
Vol 40 (No. 3) ◽  
pp. 87-93
Author(s):  
M. Švec ◽  
M. Miklovičová ◽  
V. Šudyová ◽  
M. Hudcovicová ◽  
P. Hauptvogel ◽  
...  

Resistance to powdery mildew (<I>Blumeria graminis </I>(DC.) E. O. Speer<I> </I>f.sp. <I>tritici</I> Em. Marchal) in <I>Aegilops crassa </I>Boiss.<I>, Ae. ventricosa </I>Tausch<I>, Ae. biuncialis </I>Vis.<I>, Ae. triuncialis </I>L.<I> </I>and<I> Ae. cylindrica </I>Host<I> </I>was tested at the stage of primary leaves in the years 2000 and 2001. All plants of <I>Ae. ventricosa, Ae. biuncialis </I>and sample No. 9 of <I>Ae. cylindrica</I> repeatedly showed a susceptible reaction after being inoculated by all powdery mildew isolates used. In contrast, plants of <I>Ae. crassa, </I>sample No. 8 of <I>Ae. cylindrica </I>and all samples (No. 13, 21, 22, 24 and 26) of <I>Ae. triuncialis </I>were resistant to all isolates. Samples No. 5, 6, 7, 19 and 23 of <I>Ae. cylindrica </I>contained resistant and susceptible plants in both years. Virulence to these samples ranged from 3% to 18%. Cluster analysis using DNA microsatellite markers showed that the accessions are arranged in groups based on taxonomic relationship but not on basis of resistance. Plants susceptible to powdery mildew at the juvenile stage showed satisfactory adult plant resistance.



2002 ◽  
Vol 121 (5) ◽  
pp. 407-410 ◽  
Author(s):  
C. G. Liu ◽  
Y. W. Wu ◽  
H. Hou ◽  
C. Zhang ◽  
Y. Zhang
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