Effect of Enzyme Dilution on the Relative Electrophoretic Mobility of Glutamate Dehydrogenase Isozymes in the Prairie Rattlesnake, Crotalus viridis viridis

Copeia ◽  
1992 ◽  
Vol 1992 (4) ◽  
pp. 1117 ◽  
Author(s):  
Jose M. Hernandez-Juviel ◽  
David J. Morafka ◽  
Imelda Delgado ◽  
Gary D. Scott ◽  
Robert W. Murphy
Keyword(s):  
Glutamate Dehydrogenase ◽  
Electrophoretic Mobility ◽  
Crotalus Viridis ◽  
Relative Electrophoretic Mobility

The relative electrophoretic mobility of apo(A) isoforms depends on the gel system: Proposal of a nomenclature for apo(A) phenotypes

1995 ◽  
Vol 115 ◽  
pp. S36 ◽  
Author(s):  
H.G. Kraft ◽  
A. Lingenhel ◽  
G. Bader ◽  
G.M. Kostner ◽  
G. Utermann
Keyword(s):  
Electrophoretic Mobility ◽  
Relative Electrophoretic Mobility ◽  
Gel System

The relative electrophoretic mobility of apo(a) isoforms depends on the gel system: proposal of a nomenclature for apo(a) phenotypes

1996 ◽  
Vol 125 (1) ◽  
pp. 53-61 ◽  
Author(s):  
H.G. Kraft ◽  
A. Lingenhel ◽  
G. Bader ◽  
G.M. Kostner ◽  
G. Utermann
Keyword(s):  
Electrophoretic Mobility ◽  
Relative Electrophoretic Mobility ◽  
Gel System

scholarly journals Phosphorylation and Dephosphorylation of Tau Protein by the Catalytic Subunit of PKA, as Probed by Electrophoretic Mobility Retard

2020 ◽  
pp. 1-14
Author(s):  
María J. Benítez ◽  
Raquel Cuadros ◽  
Juan S. Jiménez
Keyword(s):  
Protein Kinases ◽  
Electrophoretic Mobility ◽  
Energy State ◽  
Paired Helical Filaments ◽  
Catalytic Subunit ◽  
Relative Electrophoretic Mobility ◽  
Mitochondrial Functions ◽  
Phosphorylation Reaction ◽  
The Stability ◽  
Hyperphosphorylated Form

Background: Tau is a microtubule associated protein that regulates the stability of microtubules and the microtubule-dependent axonal transport. Its hyperphosphorylated form is one of the hallmarks of Alzheimer’s disease and other tauopathies and the major component of the paired helical filaments that form the abnormal proteinaceous tangles found in these neurodegenerative diseases. It is generally accepted that the phosphorylation extent of tau is the result of an equilibrium in the activity of protein kinases and phosphatases. Disruption of the balance between both types of enzyme activities has been assumed to be at the origin of tau hyperphosphorylation and the subsequent toxicity and progress of the disease. Objective: We explore the possibility that, beside the phosphatase action on phosphorylated tau, the catalytic subunit of PKA catalyzes both tau phosphorylation and also tau dephosphorylation, depending on the ATP/ADP ratio. Methods: We use the shift in the relative electrophoretic mobility suffered by different phosphorylated forms of tau, as a sensor of the catalytic action of the enzyme. Results: The results are in agreement with the long-known thermodynamic reversibility of the phosphorylation reaction (ATP + Protein = ADP+Phospho-Protein) catalyzed by PKA and many other protein kinases. Conclusion: The results contribute to put the compartmentalized energy state of the neuron and the mitochondrial-functions disruption upstream of tau-related pathologies.

scholarly journals Urea-Elicited Changes in Relative Electrophoretic Mobility of Certain Glycinin and β-Conglycinin Subunits

1984 ◽  
Vol 76 (3) ◽  
pp. 840-842 ◽  
Author(s):  
Elizabeth Pacheco Batista Fontes ◽  
Maurilio Aves Moreira ◽  
Corinne S. Davies ◽  
Niels C. Nielsen
Keyword(s):  
Electrophoretic Mobility ◽  
Relative Electrophoretic Mobility

Soluble Fibrin Monomer Complexes Demonstrated by Agarose Gel Filtration and by Adsorption on Insolubilized Fibrinogen

1975 ◽  
Vol 34 (01) ◽  
pp. 216-222 ◽  
Author(s):  
R von Hugo ◽  
R Hafter ◽  
A Stemberger ◽  
H Graeff
Keyword(s):  
Molecular Weight ◽  
Electrophoretic Mobility ◽  
Gel Filtration ◽  
Adsorption Chromatography ◽  
Agarose Gel ◽  
Soluble Fibrin ◽  
Relative Electrophoretic Mobility ◽  
Fibrin Monomer

SummaryIncubation of fibrinogen with small amounts of thrombin resulted in the occurrence of soluble fibrin monomer complexes. These complexes consisted predominantly of a derivative with a higher molecular weight than that of fibrinogen. It was characterized by its relative electrophoretic mobility in 5% PAA gel (0.28 × 10-5 cm2/V × sec) and its elution position prior to the fibrinogen peak following gel filtration. Using adsorption chromatography on insolubilized fibrinogen the derivative dissociated at a ratio of almost 1 : 1 into one part which was adsorbed and into fibrinogen which was not adsorbed. The part which was adsorbed seemed to be the thrombin mediated fibrin monomer. This study confirms the concept that dissociable dimeric fibrinogen-fibrin monomer complexes occur after limited action of thrombin on fibrinogen.

Changes in the relative electrophoretic mobility of higher plant tubulin subunits in SDS-polyacrylamide gels

1987 ◽  
Vol 914 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Richard J. Cyr ◽  
Michael Sotak ◽  
Mauricio M. Bustos ◽  
Mark J. Guiltinan ◽  
Donald E. Fosket
Keyword(s):  
Electrophoretic Mobility ◽  
Polyacrylamide Gels ◽  
Higher Plant ◽  
Relative Electrophoretic Mobility ◽  
Plant Tubulin ◽  
Higher Plant Tubulin

scholarly journals Uneven distribution of antitreponema antibody acitivity in differing immunoglobulin G fractions from patients with early syphilis

1980 ◽  
Vol 29 (3) ◽  
pp. 837-841
Author(s):  
J J van der Sluis ◽  
M Boer
Keyword(s):  
Electrophoretic Mobility ◽  
Immunoglobulin G ◽  
Uneven Distribution ◽  
Antibody Activity ◽  
Serological Testing ◽  
Basic Part ◽  
Relative Electrophoretic Mobility ◽  
Early Syphilis ◽  
Basic Fraction ◽  
Male Patients

Three fractions, containing immunoglobulin G's (IgG's) of differing relative electrophoretic mobility, were isolated from sera of 33 male patients with untreated early syphilis. Serological testing, employing four different serodiagnostic procedures for these fractions at equal amounts of IgG, revealed a very constant reaction pattern for all patients. In the earliest stages of the disease, the most basic fraction was the first to show antibody activity. In progressive stages, antibody activity subsequently was also found in the two less basic fractions. However, in all stages of disease studied, the basic part of the IgG made the largest contribution of the total antibody activity within the IgG class, indicating an uneven distribution of antitreponema antibody activity over heterogeneous IgG. Several possible explanations of the observations are discussed.

scholarly journals Vitamin C protects against and reverses specific hypochlorous acid- and chloramine-dependent modifications of low-density lipoprotein

2000 ◽  
Vol 346 (2) ◽  
pp. 491-499 ◽  
Author(s):  
Anitra C. CARR ◽  
Terry TIJERINA ◽  
Balz FREI
Keyword(s):  
Vitamin C ◽  
Electrophoretic Mobility ◽  
Apolipoprotein B ◽  
Hypochlorous Acid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cysteine Residues ◽  
Low Density ◽  
Relative Electrophoretic Mobility ◽  
Lysine Residues

Activated phagocytes produce the highly reactive oxidant hypochlorous acid (HOCl) via the myeloperoxidase-catalysed reaction of hydrogen peroxide with chloride ions. HOCl reacts readily with a number of susceptible targets on apolipoprotein B-100 of low-density lipoprotein (LDL), resulting in uncontrolled uptake of HOCl-modified LDL by macrophages. We have investigated the efects of vitamin C (ascorbate), an effective water-soluble antioxidant, on the HOCl- and chloramine-dependent modification of LDL. Co-incubation of vitamin C (25-200 μM) with LDL resulted in concentration-dependent protection against HOCl (25-200 μM)-mediated oxidation of tryptophan and lysine residues, formation of chloramines and increases in the relative electrophoretic mobility of LDL. Vitamin C also partially protected against oxidation of cysteine residues by HOCl, and fully protected against oxidation of these residues by the low-molecular-mass chloramines, Nα-acetyl-lysine chloramine and taurine chloramine, and to a lesser extent monochloramine (each at 25-200 μM). Further, we found that HOCl (25-200 μM)-dependent formation of chloramines on apolipoprotein B-100 was fully reversed by 200 μM vitamin C; however, the loss of lysine residues and increase in relative electrophoretic mobility of LDL were only partially reversed, and the loss of tryptophan and cysteine residues was not reversed. Time-course experiments showed that the reversal by vitamin C of HOCl-dependent modifications became less efficient as the LDL was incubated for up to 4 h at 37 °C. These data show that vitamin C not only protects against, but also reverses, specific HOCl- and chloramine-dependent modifications of LDL. As HOCl-mediated LDL modifications have been strongly implicated in the pathogenesis of atherosclerosis, our data indicate that vitamin C could contribute to the anti-atherogenic defence against HOCl.

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