plant tubulin
Recently Published Documents


TOTAL DOCUMENTS

22
(FIVE YEARS 1)

H-INDEX

11
(FIVE YEARS 0)

2021 ◽  
Author(s):  
Kunalika Jain ◽  
Megha Roy ◽  
Chaitanya A. Athale

Microtubules (MTs) are dynamic polymers vital for cellular physiology. Bulk tubulin polymerization is nucleation dependent, while individual filaments exhibit 'dynamic instability' driven by GTP hydrolysis rates. Although MTs assembled from well-studied animal brain tubulins have very comparable nucleation and GTP-hydrolysis rates, the kinetic rates of evolutionarily more distant species could diverge. Here we focus on a plant tubulin, the legume Vigna sp. (mung bean) to test the effect of kinetic diversification on MT polymerization. We activity purify tubulin from seedlings and find MT filaments are fewer and shorter than animal brain tubulin. We find mung tubulin polymerization kinetics is nucleation dependent with a high rate of GTP hydrolysis and a critical concentration lower than previously reported for tubulins. A computational model of the kinetics based on the relative influence of rates of nucleation and hydrolysis demonstrates increased rates of hydrolysis can affect MT filament numbers and their lengths, as compared to increasing nucleation rates. Our approach provides a framework to compare the effect of evolutionary diversification of MT nucleation and elongation.





2014 ◽  
Vol 48 (1) ◽  
pp. 7-14 ◽  
Author(s):  
A. Yu. Nyporko ◽  
Ya. B. Blume


Planta ◽  
2008 ◽  
Vol 229 (1) ◽  
pp. 143-150 ◽  
Author(s):  
Yaroslav Blume ◽  
Alla Yemets ◽  
Vadym Sulimenko ◽  
Tetyana Sulimenko ◽  
Jordi Chan ◽  
...  


2008 ◽  
Vol 32 (5) ◽  
pp. 571-573 ◽  
Author(s):  
D BREVIARIO ◽  
S GIANI ◽  
E PONZONI ◽  
F MASTROMAURO ◽  
L MORELLO
Keyword(s):  




PROTOPLASMA ◽  
2006 ◽  
Vol 227 (2-4) ◽  
pp. 185-196 ◽  
Author(s):  
K. Schwarzerová ◽  
J. Petrášek ◽  
K. C. S. Panigrahi ◽  
S. Zelenková ◽  
Z. Opatrný ◽  
...  


2005 ◽  
Vol 47 (4) ◽  
pp. 457-466 ◽  
Author(s):  
Chun-Hua XU ◽  
Shan-Jin HUANG ◽  
Ming YUAN


2000 ◽  
Vol 45 (24) ◽  
pp. 2258-2263 ◽  
Author(s):  
Shanjin Huang ◽  
Haiyun Ren ◽  
Ming Yuan


Sign in / Sign up

Export Citation Format

Share Document