Studies on Pasteurella multocida. IX. Levamisole-Induced Augmentation of Immune Responses to a Live Fowl Cholera Vaccine

1980 ◽  
Vol 24 (1) ◽  
pp. 71 ◽  
Author(s):  
S. K. Maheswaran ◽  
S. K. Dua ◽  
E. S. Thies
Keyword(s):  
Immune Responses ◽  
Pasteurella Multocida ◽  
Cholera Vaccine ◽  
Fowl Cholera

scholarly journals Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

2021 ◽  
Vol 8 (1) ◽  
pp. 117-124
Author(s):  
Mashuda Akter ◽  
Md Mosaraf Hossain ◽  
Md Kamrul Hassan ◽  
Ravi Yadav ◽  
Fahima Morsheda ◽  
...  
Keyword(s):  
Immune Responses ◽  
Pasteurella Multocida ◽  
Vaccine Development ◽  
Primary Vaccination ◽  
Cholera Vaccine ◽  
Post Vaccination ◽  
Passive Haemagglutination ◽  
Fowl Cholera ◽  
Group A ◽  
Group B

An experiment was conducted to investigate the immune response induced in chickens by capsular extract of Pasteurella multocida isolated from rats wandering in and around the poultry farms. The rat isolate of P. multocida was isolated and identified by cultural, morphological, and biochemical characteristics, followed by capsular extract preparation and experimental vaccine development. The isolated P. multocida was found Gram-negative, non-motile, non-spore forming rod occurring singly or pains and occasionally as chains or filaments in Gram’s-staining method. The isolates consistently produced acid from dextrose, sucrose and mannitol but not fermented maltose or lactose. The Capsular antigen was extracted and confirmed by acriflavine test. Finally, experimental fowl cholera vaccine was prepared. Primary vaccination was performed at the dose rate of 5.6×107 CFU/ml through intramuscular and subcutaneous routes in birds of group A (10 birds) and group B (10 birds) and group C (10 birds) were control birds. Secondary vaccination was similarly performed after 15 days of primary vaccination in groups A and B. The levels of pre-vaccination and post-vaccination sera were determined by passive haemagglutination test. The passive haemagglutination antibody titre was recorded on 15 and 35 days of post vaccination in groups A and B. It was demonstrated that experimental capsular extract fowl cholera vaccine conferred 100% protection (p<0.01) against challenge infection and found to be safe. It could be suggested that after thorough field trial, the experimentally prepared capsular extract FC vaccine using rat isolate of P. multocida may be used side by side with conventional FC vaccine. Res. Agric., Livest. Fish.8(1): 117-124, April 2021

scholarly journals Isolation and Identification of Pasteurella multocida from Chicken for the Preparation of Oil Adjuvanted Vaccine

2013 ◽  
Vol 2 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Samina Ievy ◽  
Mohammad Ferdousur Rahman Khan ◽  
Md Ariful Islam ◽  
Md Bahanur Rahman
Keyword(s):  
Immune Response ◽  
Pasteurella Multocida ◽  
Challenge Infection ◽  
Cholera Vaccine ◽  
Isolation And Identification ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Adjuvanted Vaccine ◽  
Group A ◽  
The Mean

The research work was performed for the isolation and identification of Pasteurella multocida from field cases, preparation of oil adjuvanted vaccine from isolated strain and determination of its efficacy. Samples were collected from suspected dead birds of three poultry farms of Bangladesh (Code name: M and R). The P. multocida isolates were Gram negative, non-motile, non- spore forming rod occurring singly or pairs and occasionally as chains or filaments. Biochemically P. multocida ferment basic sugar and consistently produced acid except from maltose and lactose. After isolation formalin killed oil adjuvanted Fowl cholera vaccine was prepared in Laboratory of the Department of Microbiology and Hygiene, BAU and this experimental vaccine (3.2x108 CFU/ml) was administered in nine weeks old White Leg Horn chickens at the different dose rate through intramuscular (IM) route in each selected group A (1ml alum precipitated vaccine), B (0.5ml alum precipitated vaccine), C (1ml oil adjuvanted vaccine) and D (0.5ml oil adjuvanted vaccine). Pre-vaccinated sera were collected from all groups of birds. The mean of Passive Hemagglutination (PHA) titers of post-vaccination were 51±17.8, 76.8±17, 89.6±17, and 115±17.81 in group A, B, C and D respectively which consist of 5 birds in each. The vaccine produced better immune response when boostering with the similar dose and route at 15 days after primary vaccination. The mean PHA titers were higher at group D than other groups after boostering. Challenge infection was conducted on all the vaccinated and control group (n=5) of birds after 15 days of vaccination which protect 93.75% of birds and the PHA titers from different groups analyzed to determine the protective capacity of vaccinated chickens against challenge exposure. It was demonstrated that experimental oil adjuvanted fowl cholera vaccine with 0.5ml dose produce higher immune response against challenge infection and found to be safe. Microbes and Health, June 2013, 2(1): 1-4DOI: http://dx.doi.org/10.3329/mh.v2i1.17253

scholarly journals Efficacy of Formalin Killed Fowl Cholera Vaccine in Experimentally Immunized Fayoumi Chickens

1970 ◽  
Vol 2 (1) ◽  
pp. 23-25
Author(s):  
MK Rahman ◽  
MB Rahman ◽  
MNA Siddiky ◽  
MA Kafi ◽  
MA Islam ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Subcutaneous Route ◽  
Cholera Vaccine ◽  
Passive Haemagglutination ◽  
Fowl Cholera ◽  
Virulent Isolate ◽  
Unvaccinated Control ◽  
Serotype 1 ◽  
Antibody Titres ◽  
Subcutaneous Inoculation

Efficacy of experimentally prepared formalin killed fowl cholera vaccine in Fayoumi chickens via different routes of vaccination was determined during the period from April 2002 to March 2003. Pasteurella multocida (PM-38) serotype 1 (X-73) was employed for vaccine preparation and antibody titres of the chicken sera were determined by passive haemagglutination (PHA) test. Vaccination was done either intramuscularly or subcutaneously. Each of the experimental chickens was challenged with a virulent isolate of P. multocida @ 3.8 x 108 CFU / ml per bird intramuscularly. The 100% vaccinated chickens protected against virulent P. multocida infection but all (100%) unvaccinated control birds died within 72 hours of challenge. Intramuscular (both primary and booster) route of vaccinations was found superior and more effective than subcutaneous route of inoculation. The higher PHA antibody titre was recorded with intramuscularly (222.86 ± 25.60) than subcutaneously (111.43 ± 12.80) vaccinated groups of birds. The result revealed the fact that intramuscular route followed by subcutaneous inoculation could be done for immunization against fowl cholera in chickens. Key words: Efficacy; fowl cholera vaccine; formalin killed; Fayoumi chicken doi: 10.3329/bjvm.v2i1.1929 Bangl. J. Vet. Med. (2004). 2 (1) : 23-25

Attenuated Live Fowl Cholera Vaccine I. Development of Vaccine Strain M3G of Pasteurella multocida

1979 ◽  
Vol 23 (4) ◽  
pp. 863 ◽  
Author(s):  
I. Hertman ◽  
J. Markenson ◽  
A. Michael ◽  
E. Geier
Keyword(s):  
Vaccine Strain ◽  
Pasteurella Multocida ◽  
Cholera Vaccine ◽  
Fowl Cholera

scholarly journals Immunogenicity of capsular extract prepared from a local duck isolate of Pasteurella multocida

1970 ◽  
Vol 6 (1) ◽  
pp. 19-22
Author(s):  
M Sukul ◽  
MSR Khan ◽  
MT Rahman ◽  
K Begum
Keyword(s):  
Pasteurella Multocida ◽  
Laboratory Animals ◽  
Serological Response ◽  
Cholera Vaccine ◽  
Serum Samples ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Capsular Antigen ◽  
Group A ◽  
Group B

The immunogenic response and protection of duck with capsular extract of local isolate of duck cholera organism, i.e. Pasteurella multocida was compared with that of conventional fowl cholera vaccine prepared in Bangladesh Agricultural University, Mymensingh. Feces, liver, heart, lungs, trachea and intestinal swabs were collected from a total of 50 sick and apparently healthy ducks. The duck cholera organisms were isolated and characterized by their cultural, physiochemical, staining properties and laboratory animals and also duck and ducklings inoculation. Capsular antigen was prepared from this isolate. Twelve weeks aged ducks of Jinding breeds were divided into three groups such as A, B and C. Each duck of group A was inoculated with 1 ml of capsular antigen containing 200 µl capsular protein and 1 ml of Bangladesh Agricultural University fowl cholera vaccine in group B intramuscularly followed by second vaccination with same vaccine with similar dose and route at 15 days interval in group A and B respectively. Ducks of group C were kept as control. Sera samples of each of the ducks of all the three groups were collected at 7 and 14 days post-vaccination following each of the primary and secondary vaccination. Each of the serum samples of all the three groups of birds was titrated by using passive hemagglutination (PHA) test and results recorded that both the capsular extract (96.00 ± 45.25) and fowl cholera vaccine (108 ± 89.37) produced more or less similar serological response at two weeks after secondary vaccination. Protection test was performed with all birds of three groups after 21 days of secondary vaccination and the results showed that ducks of both group A and B conferred 100% protection. Key words: Pasteurella multocida, capsular extract, fowl cholera vaccine, duck, immunogenicity DOI = 10.3329/bjvm.v6i1.1333 Bangl. J. Vet. Med. (2008). 6 (1): 19-22

Uses of single dose dependent and relative potency assays for the evaluation of inactivated fowl cholera vaccine

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Selim S Salama ◽  
Fatma M ◽  
Gadallah Fatma El Zahraa G ◽  
Abo Elkhir ◽  
Afaf A Khedrand ◽  
...  
Keyword(s):  
Single Dose ◽  
Relative Potency ◽  
Cholera Vaccine ◽  
Fowl Cholera ◽  
Dose Dependent
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