scholarly journals Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

2021 ◽  
Vol 8 (1) ◽  
pp. 117-124
Author(s):  
Mashuda Akter ◽  
Md Mosaraf Hossain ◽  
Md Kamrul Hassan ◽  
Ravi Yadav ◽  
Fahima Morsheda ◽  
...  
Keyword(s):  
Immune Responses ◽  
Pasteurella Multocida ◽  
Vaccine Development ◽  
Primary Vaccination ◽  
Cholera Vaccine ◽  
Post Vaccination ◽  
Passive Haemagglutination ◽  
Fowl Cholera ◽  
Group A ◽  
Group B

An experiment was conducted to investigate the immune response induced in chickens by capsular extract of Pasteurella multocida isolated from rats wandering in and around the poultry farms. The rat isolate of P. multocida was isolated and identified by cultural, morphological, and biochemical characteristics, followed by capsular extract preparation and experimental vaccine development. The isolated P. multocida was found Gram-negative, non-motile, non-spore forming rod occurring singly or pains and occasionally as chains or filaments in Gram’s-staining method. The isolates consistently produced acid from dextrose, sucrose and mannitol but not fermented maltose or lactose. The Capsular antigen was extracted and confirmed by acriflavine test. Finally, experimental fowl cholera vaccine was prepared. Primary vaccination was performed at the dose rate of 5.6×107 CFU/ml through intramuscular and subcutaneous routes in birds of group A (10 birds) and group B (10 birds) and group C (10 birds) were control birds. Secondary vaccination was similarly performed after 15 days of primary vaccination in groups A and B. The levels of pre-vaccination and post-vaccination sera were determined by passive haemagglutination test. The passive haemagglutination antibody titre was recorded on 15 and 35 days of post vaccination in groups A and B. It was demonstrated that experimental capsular extract fowl cholera vaccine conferred 100% protection (p<0.01) against challenge infection and found to be safe. It could be suggested that after thorough field trial, the experimentally prepared capsular extract FC vaccine using rat isolate of P. multocida may be used side by side with conventional FC vaccine. Res. Agric., Livest. Fish.8(1): 117-124, April 2021

scholarly journals THE EFFICACY OF VACCINATION OF LAYER CHICKENS WITH INACTIVATED FOWL CHOLERA BACTERIN PREPARED FROM LOCAL EGYPTIAN STRAINS OF Pasteurella multocida

2020 ◽  
Vol 57 (4) ◽  
Author(s):  
Wafaa Abd El-Ghany ◽  
Hanan Ali Ahmed ◽  
Ali Zaher Qandoos ◽  
Mohamed Abd El-Rahman Bosila Bosila
Keyword(s):  
Pasteurella Multocida ◽  
Histopathological Examination ◽  
Clinical Signs ◽  
Post Vaccination ◽  
Fowl Cholera ◽  
Humoral Immune ◽  
Group A ◽  
The Mean ◽  
Layer Chickens ◽  
Group B

This study was carried out to evaluate the efficacy of vaccination of layer chickens with inactivated FC bacterin prepared from local Egyptian strains of Pasteurella multocida (P. multocida). A total of 200 layer chickens were divided into 5 equal groups, 40 for each. At the age of 6 weeks, chickens in groups (A) and (B) were vaccinated with P. multocida serotypes A:1 and A:3, respectively, booster doses were given after 3 weeks (9 weeks old) and challenge was done with virulent serotypes A:1 and A:3 at 2 weeks later (11 weeks old). Chickens in groups (C) and (D) were not vaccinated, only challenged with P. multocida serotype A:1 and A:3, respectively. Birds in group (E) were kept as non-vaccinated and non-challenged. Blood samples were collected weekly from all groups for humoral immune response. All the birds were kept under observation for signs, mortalities, lesions and re-isolation of challenging organism and for histopathological examination. Results of the mean Enzyme Linked Immuno-Sorbent Assay (ELISA) revealed that the highest level was at 5 weeks post vaccination as the titers reached to 3970 in group (A) and 3905 in group (B). The clinical signs, mortality rate and lesions were mild in the vaccinated birds while severe lesions were in non-vaccinated and challenged birds. The protection rates were 85 % and 80 % in groups (A) and (B); respectively, while 10 % and 20 % in groups (C) and (D); respectively. The re-isolation rates of P. multocida after challenge were 95 % and 90 % in non-vaccinated-challenged birds with P. multocida serotypes A:1 and A:3; respectively, while they were 25 % and 15 % in vaccinated-challenged groups with P. multocida serotypes A:1 and A:3; respectively. Histopathological examination of P. multocida vaccinated-challenged birds revealed mild to no microscopic lesions when compared with non-vaccinated challenged chickens. In conclusion, the prepared FC inactivated bacterin from the local Egyptian predominant P. multocida serovars proved efficacy and protection of layer chickens. Key words: Pasteurella multocida; chickens; immunization; protection; Egypt UČINKOVITOST CEPLJENJA KOKOŠI NESNIC Z INAKTIVIRANO BAKTERIJO KOLERE PERJADI, PRIPRAVLJENE IZ LOKALNIH EGIPTOVSKIH SEVOV BAKTERIJE Pasteurella multocida Povzetek: Raziskava je bila izvedena z namenom ocenitve učinkovitosti cepljenja kokoši nesnic z inaktivirano bakterijo FC, pripravljeno iz lokalnih egiptovskih sevov bakterije Pasteurella multocida (P. multocida). Skupno 200 kokoši nesnic je bilo razdeljenih v 5 enakih skupin. V vsaki skupini je bilo 40 kokoši. Pri 6 tednih smo kokoši v skupinah A in B cepili s serotipoma P. multocida A:1 in A:3, po 3 tednih, ko so bile živali stare 9 tednov, so dobile poživitvene doze cepiva. Po dveh tednih (v starosti 11 tednov) so bile kokoši okužene z virulentnima serotipoma A:1 in A:3. Piščanci v skupinah C in D niso bili cepljeni temveč samo okuženi s serotipoma A:1 in A:3. Kokoši v skupini E niso bile niti cepljene, niti okužene. Vzorci krvi so bili odvzeti pri vseh skupinah tedensko za preverjanje humoralnega imunskega odziva. Vse kokoši smo stalno opazovali in beležili prisotnost bolezenskih znakov, različnih ran in umiranje kokoši. Pri poginulih kokoših smo osamili bakterije ter opravili histopatološki pregled. Rezultati encimsko-imunskega testa (ELISA) so pokazali da je bila najvišja stopnja zaščite dosežena 5 tednov po cepljenju, saj so titri dosegli 3970 v skupini A in 3905 v skupini B. Klinični znaki, stopnja umrljivosti in rane so bili pri cepljenih kokoših blagi, hude rane pa so bile vidne pri necepljenih in okuženih kokoših. Stopnja zaščite je bila v skupinah A in B 85- oziroma 80-odstotna, v skupinah C in D pa 10- oziroma 20-odstotna. Stopnje ponovne izolacije P. multocida po okužbi so bile 90 in 95 odstotkov pri kokoših, ki niso bile cepljene, medtem, ko so bile v skupinah, ki so bile okužene s P. multocida serotipa A:1 in A:3 15- in 25-odstotkov. Histopatološki pregled cepljenih in okuženih kokoši je pokazal popolno odsotnost ali prisotnost blagih mikroskopskih poškodb, medtem ko so imele necepljene okužene kokoši bolj obsežne histopatološke poškodbe. Pripravljena inaktivirana bakterija FC iz lokalnih egiptovskih prevladujočih serovarov P. multocide se je izkazala za učinkovito zaščito kokoši nesnic.Ključne besede: Pasteurella multocida; kokoši; imunizacija; zaščita; Egipt

scholarly journals Comparative immunogenicity of fowl cholera vaccine in Jinding ducks

2014 ◽  
Vol 30 (2) ◽  
pp. 41-45 ◽  
Author(s):  
S Sultana ◽  
S Saha ◽  
MM Amin
Keyword(s):  
Pasteurella Multocida ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Challenge Infection ◽  
Fowl Cholera ◽  
Significant Difference ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Group A ◽  
Group B

This study compared the immunogenicity of alum-precipitated formalin-killed fowl cholera vaccines (BAU-FCV and LRI-FCV) in Jinding ducks. The ducks were divided into three groups (A = 14, B = 14, C = 12). Group A was inoculated with BAU- FCV 0.5 mL and group B with LRI- FCV 1.0 mL intramuscularly (im) at the age of six weeks and group C served as unvaccinated control. Booster vaccination was administered similarly at 11 weeks of age in groups A and B. Challenge infection was given to all birds two weeks after booster vaccination. Passive Haemagglutination Assay (PHA) antibody titres in group A were 59.4 ± 4.6 21 days after primary vaccination, 137.1 ± 21.8 15 days after booster vaccination, 100.6 ± 12.9 21 days after booster vaccination, and 256.0 ± 48.4 15 days after challenge. In group B, titres were 50.3 ± 6.5, 118.9 ± 9.1, 91.4 ± 12.9, 237.7 ± 51.7, respectively, whereas titres in group C remained at ?4.0 ± 0.0. The antibody titres were insignificant when compared between pre-vaccination and 21 days after primary vaccination in both vaccinated groups (A and B). PHA antibody titres of groups A were significantly (P < 0.0001) increased at 15 days after booster and in case of group B the antibody titres were insignificant. At 15 days after challenge the antibody titres were highly significant in both groups (A and B). There was no significant difference between the two vaccinated groups. Following challenge infection with virulent Pasteurella multocida 88.9% of birds vaccinated with BAU-FCV, and 77.8% of birds vaccinated with LRI-FCV survived, while all unvaccinated birds died. Both vaccines were safe and effective. DOI: http://dx.doi.org/10.3329/bvet.v30i2.18253 Bangl. vet. 2013. Vol. 30, No. 2, 41-45

scholarly journals Immunogenicity of capsular extract prepared from a local duck isolate of Pasteurella multocida

1970 ◽  
Vol 6 (1) ◽  
pp. 19-22
Author(s):  
M Sukul ◽  
MSR Khan ◽  
MT Rahman ◽  
K Begum
Keyword(s):  
Pasteurella Multocida ◽  
Laboratory Animals ◽  
Serological Response ◽  
Cholera Vaccine ◽  
Serum Samples ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Capsular Antigen ◽  
Group A ◽  
Group B

The immunogenic response and protection of duck with capsular extract of local isolate of duck cholera organism, i.e. Pasteurella multocida was compared with that of conventional fowl cholera vaccine prepared in Bangladesh Agricultural University, Mymensingh. Feces, liver, heart, lungs, trachea and intestinal swabs were collected from a total of 50 sick and apparently healthy ducks. The duck cholera organisms were isolated and characterized by their cultural, physiochemical, staining properties and laboratory animals and also duck and ducklings inoculation. Capsular antigen was prepared from this isolate. Twelve weeks aged ducks of Jinding breeds were divided into three groups such as A, B and C. Each duck of group A was inoculated with 1 ml of capsular antigen containing 200 µl capsular protein and 1 ml of Bangladesh Agricultural University fowl cholera vaccine in group B intramuscularly followed by second vaccination with same vaccine with similar dose and route at 15 days interval in group A and B respectively. Ducks of group C were kept as control. Sera samples of each of the ducks of all the three groups were collected at 7 and 14 days post-vaccination following each of the primary and secondary vaccination. Each of the serum samples of all the three groups of birds was titrated by using passive hemagglutination (PHA) test and results recorded that both the capsular extract (96.00 ± 45.25) and fowl cholera vaccine (108 ± 89.37) produced more or less similar serological response at two weeks after secondary vaccination. Protection test was performed with all birds of three groups after 21 days of secondary vaccination and the results showed that ducks of both group A and B conferred 100% protection. Key words: Pasteurella multocida, capsular extract, fowl cholera vaccine, duck, immunogenicity DOI = 10.3329/bjvm.v6i1.1333 Bangl. J. Vet. Med. (2008). 6 (1): 19-22

scholarly journals Isolation and Identification of Pasteurella multocida from Chicken for the Preparation of Oil Adjuvanted Vaccine

2013 ◽  
Vol 2 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Samina Ievy ◽  
Mohammad Ferdousur Rahman Khan ◽  
Md Ariful Islam ◽  
Md Bahanur Rahman
Keyword(s):  
Immune Response ◽  
Pasteurella Multocida ◽  
Challenge Infection ◽  
Cholera Vaccine ◽  
Isolation And Identification ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Adjuvanted Vaccine ◽  
Group A ◽  
The Mean

The research work was performed for the isolation and identification of Pasteurella multocida from field cases, preparation of oil adjuvanted vaccine from isolated strain and determination of its efficacy. Samples were collected from suspected dead birds of three poultry farms of Bangladesh (Code name: M and R). The P. multocida isolates were Gram negative, non-motile, non- spore forming rod occurring singly or pairs and occasionally as chains or filaments. Biochemically P. multocida ferment basic sugar and consistently produced acid except from maltose and lactose. After isolation formalin killed oil adjuvanted Fowl cholera vaccine was prepared in Laboratory of the Department of Microbiology and Hygiene, BAU and this experimental vaccine (3.2x108 CFU/ml) was administered in nine weeks old White Leg Horn chickens at the different dose rate through intramuscular (IM) route in each selected group A (1ml alum precipitated vaccine), B (0.5ml alum precipitated vaccine), C (1ml oil adjuvanted vaccine) and D (0.5ml oil adjuvanted vaccine). Pre-vaccinated sera were collected from all groups of birds. The mean of Passive Hemagglutination (PHA) titers of post-vaccination were 51±17.8, 76.8±17, 89.6±17, and 115±17.81 in group A, B, C and D respectively which consist of 5 birds in each. The vaccine produced better immune response when boostering with the similar dose and route at 15 days after primary vaccination. The mean PHA titers were higher at group D than other groups after boostering. Challenge infection was conducted on all the vaccinated and control group (n=5) of birds after 15 days of vaccination which protect 93.75% of birds and the PHA titers from different groups analyzed to determine the protective capacity of vaccinated chickens against challenge exposure. It was demonstrated that experimental oil adjuvanted fowl cholera vaccine with 0.5ml dose produce higher immune response against challenge infection and found to be safe. Microbes and Health, June 2013, 2(1): 1-4DOI: http://dx.doi.org/10.3329/mh.v2i1.17253

scholarly journals Comparative efficacy of BAU-fowl cholera and DLS-fowl cholera vaccines in indigenous chicken

2018 ◽  
Vol 5 (2) ◽  
pp. 193-199
Author(s):  
Chamak Nahar Shampa ◽  
Suma Akter ◽  
Sukumar Saha ◽  
Md Hadiuzzaman ◽  
Azhar Ul Alam ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Research Work ◽  
Booster Vaccination ◽  
Indigenous Chicken ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Unvaccinated Control ◽  
Group A ◽  
The Mean ◽  
Group B

The present study was conducted to determine the immune response induced in indigenous chicken produced against BAU-FC and DLS-FC vaccines with their efficacy study against Pasteurella multocida. A total of forty (40) chickens were selected and divided into Group A (15), Group B (15) and Group C (10). Group A and B were vaccinated with BAU-FCV and DLS-FCV, respectively at the dose rate of 0.5 ml through SC at six weeks of age followed by boostering at 10 weeks of age while Group C was kept as unvaccinated control. Sera samples were collected after primary and booster vaccination and antibody titre was determined by Passive hemagglutination (PHA) test. The mean PHA titres recorded at 4 weeks after primary vaccination was 51.20 ± 7.84 in birds of group A and 38.40 ± 6.40 in birds of Group B. After booster vaccination, mean PHA titer was found 140.80 ± 31.35 at 16 weeks of age in case of BAU-FC vaccinated group and 115.20 ± 12.80 in case of DLS-FC vaccinated group. The mean PHA titer was 204.80 ± 31.35 and 179.20 ± 31.35 at 19 weeks of age in birds of BAU-FC and DLS-FC vaccinated group, respectively. Birds of all groups were challenged with virulent P. multocida at 17 weeks of age. It was observed that vaccinated chickens showed maximal resistance (100%) following challenge with virulent whereas unvaccinated control birds failed to resist the challenge infection. It can be assumed from the findings of present research work that both BAU-FCV and DLS-FCV are able to protect indigenous chicken from the outbreak of avian pasteurellosis and BAU-FV vaccine showed relatively higher immuno-protective titre than that of DLS-FC vaccine.Res. Agric., Livest. Fish.5(2): 193-199, August 2018

scholarly journals Efficacy of Formalin Killed Fowl Cholera Vaccine in Experimentally Immunized Fayoumi Chickens

1970 ◽  
Vol 2 (1) ◽  
pp. 23-25
Author(s):  
MK Rahman ◽  
MB Rahman ◽  
MNA Siddiky ◽  
MA Kafi ◽  
MA Islam ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Subcutaneous Route ◽  
Cholera Vaccine ◽  
Passive Haemagglutination ◽  
Fowl Cholera ◽  
Virulent Isolate ◽  
Unvaccinated Control ◽  
Serotype 1 ◽  
Antibody Titres ◽  
Subcutaneous Inoculation

Efficacy of experimentally prepared formalin killed fowl cholera vaccine in Fayoumi chickens via different routes of vaccination was determined during the period from April 2002 to March 2003. Pasteurella multocida (PM-38) serotype 1 (X-73) was employed for vaccine preparation and antibody titres of the chicken sera were determined by passive haemagglutination (PHA) test. Vaccination was done either intramuscularly or subcutaneously. Each of the experimental chickens was challenged with a virulent isolate of P. multocida @ 3.8 x 108 CFU / ml per bird intramuscularly. The 100% vaccinated chickens protected against virulent P. multocida infection but all (100%) unvaccinated control birds died within 72 hours of challenge. Intramuscular (both primary and booster) route of vaccinations was found superior and more effective than subcutaneous route of inoculation. The higher PHA antibody titre was recorded with intramuscularly (222.86 ± 25.60) than subcutaneously (111.43 ± 12.80) vaccinated groups of birds. The result revealed the fact that intramuscular route followed by subcutaneous inoculation could be done for immunization against fowl cholera in chickens. Key words: Efficacy; fowl cholera vaccine; formalin killed; Fayoumi chicken doi: 10.3329/bjvm.v2i1.1929 Bangl. J. Vet. Med. (2004). 2 (1) : 23-25

scholarly journals Comparative efficacy of BAU-Fowl cholera and DLS-Fowl cholera vaccines in indigenous ducks

2019 ◽  
Author(s):  
S. Akter ◽  
C. N. Shampa ◽  
M. A. Islam ◽  
A. U. Alam ◽  
M. Hadiuzzaman ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Booster Vaccination ◽  
Significant Loss ◽  
Challenge Infection ◽  
Fowl Cholera ◽  
Antibody Titres ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Cholera Vaccines

Background: Duck cholera is an acute, fatal, septicemic disease of domestic ducks which is responsible for significant loss in duck population. The present study was conducted to compare the immunogenicity of two formalin killed fowl cholera vaccines (BAU-FCV and DLS-FCV) in indigenous ducks. Methods: The experimental ducks were divided into three groups (A=15, B=15 and C =10 ducks) of which birds of Group A and Group B were inoculated with 0.5 ml of BAU-FCV and DLS-FCV, respectively through subcutaneous route at the age of 10 weeks whereas ducks of group C were kept as unvaccinated control. Booster vaccination was done with same dose and route at 14 weeks of age. Challenge infection was conducted after 2 weeks of booster vaccination. Results: The mean PHA antibody titres on 15 days post vaccination (DPPV), 28 DPPV, 15 days postsecondary vaccination (DPSV), 28 DPSV and 15 days post challenge were 25.60 ± 3.92, 51.20 ± 7.84, 89.60 ± 15.68, 166.40 ± 38.40 and 204.80 ± 31.35, respectively in ducks of Group A whereas, the mean antibody titres in ducks of Group B were 25.60 ± 3.92, 44.80 ± 7.84, 64.00 ± 7.53,102.40 ± 15.68 and 179.20 ± 31.35 at 15 DPPV, 28 DPPV, 15 DPSV, 28 DPSV and 15 days after challenge, respectively. In this investigation, slightly higher immune responses were observed in ducks of Group A vaccinated with BAU-FCV compare to ducks of Group B vaccinated with DLS-FCV. Birds of both vaccinated groups conferred 100% protection against challenge infection with virulent Pasteurella multocida whereas, 100% mortality was observed in control ducks after challenge. Conclusion: Both vaccines were found to be safe and effective for the vaccination of indigenous ducks against duck cholera.

scholarly journals PREPARATION AND EVALUATION OF CHEMICALLY INACTIVATED SALMONELLA ENTERITIDIS VACCINE IN CHICKENS

2017 ◽  
Vol 10 (11) ◽  
pp. 341
Author(s):  
Mounir M El-safty ◽  
Hala Mahmoud ◽  
Eman Sa Zaki ◽  
Howaida I Abd-alla
Keyword(s):  
Immune Responses ◽  
Salmonella Enteritidis ◽  
Serum Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Chemically Induced ◽  
Before And After ◽  
Group A ◽  
Group B ◽  
Cell Envelopes

  Objective: Salmonella enteritidis ghosts (SEGs) is a non-living empty bacterial cell envelopes which were generated using a different concentration of sodium hydroxide (NaOH) 6.4 mg/mL and evaluated as a vaccine candidate in specific pathogen-free (SPF) chicken. SEGs have been produced by chemical-mediated lysis and evaluated the potential efficacy of chemically induced SEG vaccine and its ability to induce protective immune responses against virulent S. enteritidis challenge in SPF chickens.Methods: SPF chickens were divided into three groups: Group A (non-vaccinated control), Group B (vaccinated with prepared vaccine), and Group C (vaccinated with commercial vaccine).Results: Vaccination of SPF chicken with SEGs induced higher immune responses before and after virulent challenge. SPF chicken vaccinated with SEGs showed increasing in serum enzyme-linked immunosorbent assay (ELISA) antibodies. During the vaccination period, Groups B and C showed higher serum antibody titer compared to Group A. The minimal inhibitory concentration (MIC) of NaOH was capable of inducing non-living SEGs, and it has successfully generated non-living SEGs by MIC of NaOH.Conclusion: It is a one-step process which means easy manufacturing and low production cost compared to protein E-mediated lysis method. Chemically induced SEG vaccine is a highly effective method for inducing protective immunity. This study strongly suggests that SEGs will be a permissive vaccine, as the method of inhibition of S. enteritidis was safe and cheaper than other methods, and it gave a good protection.

scholarly journals Effect of intranasal recombinant Mannheimia haemolytica vaccination on some haematological indices of goats infected with peste des petits ruminants virus

1970 ◽  
Vol 13 (1) ◽  
pp. 48-52
Author(s):  
OZ Tenuche ◽  
BO Emikpe
Keyword(s):  
Lymphocyte Count ◽  
Mannheimia Haemolytica ◽  
Control Group ◽  
Peste Des Petits Ruminants ◽  
Post Vaccination ◽  
Group A ◽  
Haematological Changes ◽  
Ppr Virus ◽  
Group B ◽  
Group D

There is dearth of information on the haematological changes associated with Mannheimia haemolytica vaccination in goats, hence this report which describes some haematological changes observed following vaccination with intranasal Recombinant Mannheimia haemolytica vaccine in goats naturally infected with peste des petits ruminants (PPR) virus. Twenty one (male, n=11; and female, n=10) goats were assigned to three vaccinated groups (A, B and D) with five goats per group (male: 3, female: 2), while the control group had 6 goats. Group A was vaccinated once intranasally, group B was vaccinated intranasally twice at one week interval and group D was vaccinated intranasally twice at two weeks interval . The control group (C) was not vaccinated. The vaccinated and control groups were challenged by comingling with pneumonic goats to simulate the field experience. PPR virus infection was later diagnosed in all the groups post vaccination. An average of four animals per treatment group in post-vaccination days were bled once weekly for six weeks (every week) to evaluate some haematological changes. The PCV values were within the normal range, while there was a decline in lymphocyte count at week 5, and a steady increase in neutrophil count in group A. In Group B, there was similar decline in lymphocyte count from the sixth week, while in groups C (Control) and D, the lymphocyte count declined at the 7th week, as the neutrophil counts increased. There were no significant changes in monocyte and eosinophil counts. The degree of changes in lymphocyte and neutrophil counts was mild in group B and marked in group D. This study revealed that intranasal vaccination of recombinant Mannheimia  haemolytica vaccine in the presence of PPR virus outbreak results in mild hematological derangement when the goats were vaccinated with  Mannheimia haemolytica bacterin at a week interval.Keywords: Goats, Haematology, Intranasal Recombinant Mannheimia haemolytica, Peste des Petits Ruminants, Vaccination

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