Status of the buffalo milk trade and dairy manufacturing business at Bhola district of Bangladesh, and opportunities for buffalo milk products branding

The objective of this study was to know the existing buffalo milk trade and dairy products production status along with scope for buffalo milk product branding. Hence, a face-to-face interview with eighteen milk traders and processors were performed through simple random sampling method. Milk traders and processors were above 25 years old (94%) and above 5 years (89%) experienced in dairy business. The findings exposed that 11% of the participants were involved in milk collection and selling, 39% were directly involved in product manufacturing, and 50% were engaged in milk collection and processing. Among the manufactured dairy products, traditionally made fermented doi was the best - selling dairy product in the Bhola district. About 47% participants desired to incorporate fat-rich dairy products into their production processes. In general, milk traders sold the maximum amount of milk monthly to doi manufacturers (1453 L), followed by local customers (1175 L), sweetmeat manufacturers (1000 L), restaurants (257 L), and household consumers (250 L). Results showed that buffalo milk prices varied significantly (P<0.001) over the year. The peak buffalo milk price (122 BDT/L) was recorded between November and December, and the off-peak price (82 BDT/L) was between March to May. Data indicated that about 43-50 BDT profit derived through per kg doi and sweetmeat selling. Milk traders and processors mentioned that studied areas had greater shortage of milk preservation facilities. Overall, the findings of this study may give some context for developing sustainable buffalo milk and dairy products value chain in Bangladesh. Res. Agric., Livest. Fish.8(3): 301-310, December 2021

Pre-extension demonstration of field pea varieties in selected districts of Bale highlands, Oromiya national regional state, Ethiopia

Pre extension demonstration of improved field pea varieties was conducted in Goba, Sinana and Agarfa districts of Bale zone. The main objective of the study was to demonstrate and evaluate recently released (Weyib) variety along with standard check. The demonstration was under taken on single plot of 10mx10m area for each variety with the spacing of 30cm between rows and recommended seed rate of 75kg/ha and fertilizer rate of 100kg/ha NPS. Mini-field day involving different stakeholders was organized at each respective site. Yield data per plot was recorded and analysed using descriptive statistics, while farmers’ preference to the demonstrated varieties was identified using focused group discussion and summarized using pair wise ranking methods. The demonstration result revealed that Weyib variety performed better than the standard check (Tulu shanan variety) with an average yield of 34.47qt/ha, while that of the standard check was27.26qt/ha. Weyib variety had 17.27% yield advantage over the standard check. Thus, Weyib variety was recommended for further scaling up. Res. Agric., Livest. Fish.8(3): 267-271, December 2021

Length-length relationships of elongate glass perchlet, Chanda nama (HAMILTON, 1822) from the old Brahmaputra river, Bangladesh

The generalized length-length relationships of Chanda nama ((Hamilton, 1822) belongs to Ambassidae were studied separately for a period of a calendar year collected from the Old Brahmaputra River, Mymensingh, Bangladesh for male, female and combined populations. A total of 1170 specimens were examined where 599 were male and 571 were female. The standard length, fork length and total length of male ranged from 19 to 79 mm, from 23 to 90 mm, and 28 to 100 mm respectively. The standard length, fork length and total length of female were found from 15 to 81 mm, 24 to 89 mm, and from 29 to 100 mm respectively. The generalized relationships of standard length and fork length, fork length and total length, and standard length and total length of male, female and combined populations were FL = 1.08 SL+1.41, TL = 1.11 FL + 1.54 and TL = 1.20 SL+2.81; FL = 1.09 SL+1.23, TL = 1.12 FL+1.04 and TL = 1.22 SL+2.22; and FL = 1.09 SL+1.20, TL = 1.11 FL+1.27 and TL = 1.22 SL+2.38 respectively. The coefficient of determination (R2) revealed high values in all regression analyses. In length–length relationships, the coefficient of determination (R2) ranged from 0.973-0.990. The present findings of this study will be helpful for a well-organized and significant exploitation and regulation of the Chanda nama fishery in the Old Brahmaputra River and surrounding ecosystems. Res. Agric., Livest. Fish.8(3): 339-347, December 2021

Effect of variety and nitrogen fertilizer managements on the growth and yield of Transplant aman rice managements

Varietal selection and appropriate placement of nitrogen fertilizer could reduce the magnitude of nitrogen losses to a considerable extent and improve its use efficiency for better grain production. An experiment was carried out during the period from July to December at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh to evaluate the effect of variety and nitrogen fertilizer management on the growth and yield of transplant aman rice. The treatments consisted of three varieties viz. BR11, BRRI dhan49 and Binadhan-7, and five nitrogen fertilizer managements viz. two depth of placement of USG (Urea Super Granule) and three split applications of prilled urea. The experiment was laid out in a randomized complete block design with three replications. The results showed that the effect of variety and nitrogen fertilizer management significantly influenced all the yield contributing characters except weight of 1000- grains, non-effective tillers hill-1 and panicle length. The highest grain yield (3.69 t ha-1) was obtained from Binadhan-7. In the case of nitrogen fertilizer management, the highest grain yield (4.32 t ha-1) was found from the 6 cm depth placement of USG. The highest grain yield (4.82 t ha- 1) was observed from the interaction of Binadhan-7 with the 6 cm depth of placement of USG. It may be concluded that Binadhan-7 with 6 cm depth of placement of USG appeared as the promising practice to maximize the yield of transplant aman rice. Res. Agric., Livest. Fish.8(3): 281-289, December 2021

Production performance and carcass characteristics of broiler treated with graded level of acetic acid

To investigate the effect of graded level of acetic acid on the production performance and carcass characteristics of broiler, present study was conducted for a period of 35 days in a controlled shed. A total of 240 Cobb 500 day old broiler chicks were divided into four dietary treatment groups (T0, T1, T2 and T3), each group with three replications and each replication having 20 birds. Control group (T0) birds were fed with the basal diet without any acetic acid. Birds of T1, T2 and T3 group were treated with 0.1%, 0.2% and 0.3% acetic acid in drinking water, respectively along with basal diets. Recorded data were analysed in SPSS software by using ANOVA and significance level was considered as 5%. Results showed that, acetic acid has significantly (P<0.05) improved live weight, live weight gain, feed conversion ratio (FCR) and some meat characteristics (carcass weight, thigh weight and breast weight) of experimental broilers. However, there were no significant (P>0.05) differences were observed between control group and acetic acid treated groups of birds in terms of feed intake, dressing percentage, liver weight, heart weight and abdominal fat. Net profit was higher in birds treated with acetic acid than the control group and the best performances were observed in the birds treated with 0.3% acetic acid. Res. Agric., Livest. Fish.8(3): 311-319, December 2021

Performance of double grained rice cultivar under different plant spacing

Plant spacing ensures uniform and optimum plant population and facilitates sufficient natural resources for crop growth which, in turns influences the crop yield positively. An investigation was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, during July to December 2017 to study the influence of plant spacing on the yield of double grained rice cultivar. The study comprised five spacing viz. 25 cm × 15 cm, 25 cm × 10 cm, 20 cm × 15 cm, 20 cm × 10 cm and 15 cm × 15 cm and two rice cultivars viz. a double grained rice cultivar and BRRI dhan49 and a double grained rice cultivar. It was laid out in a randomized complete block design with three replications. The double grained rice cultivar produced taller plant (155.65 cm), longer panicle (23.93 cm), higher 1000-grain weight (25.96) and higher straw yield (6.90 t ha-1), though the higher grain yield (3.68 t ha-1) was found in the test cultivar (BRRI dhan49). Planting at 20 cm × 15 cm produced the highest grain yield (3.70 t ha-1) which was at par with 25 cm × 15 cm, 25 cm × 10 cm and 20 cm × 10 cm while planting at 15 cm × 15 cm produced the lowest grain yield (3.13 t ha-1). The interaction of the double grained rice cultivar with all spacing combinations produced taller plants than the spacing combinations with BRRI dhan49. The higher grain yield (3.52 t ha-1) was found in the double grained rice cultivar with 20 cm × 10 cm spacing which was at par with the spacing of 20 cm × 15 cm and 25 cm × 15 cm, respectively; although BRRI dhan49 always produced the higher yield. The lowest grain yield (3.08 t ha-1) was recorded at the double grained rice cultivar with 25 cm × 10 cm spacing, however, the same treatment produced the highest biological yield (11.23 t ha-1) due to the highest straw yield (8.15 t ha-1). Hence, the spacing 20 cm × 15 cm might be recommended for the higher grain yield in the double grained rice cultivar Res. Agric., Livest. Fish.8(3): 273-279, December 2021

Nutritional comparison between hydroponically grown leguminous and non-leguminous fodder to identify the adaptability and best harvesting time for sustainable hydroponic grass production

Two types of fodder crops were evaluated in this study, maize (Zia maize) and black gram (BG) (Vigna mungo) and in maize, non-descriptive local maize (LM) and hybrid maize (HM) verity were used. LM was considered as control and changes of the nutritional composition according to the type of fodder (leguminous and non-leguminous) and the day was evaluated. For comparative nutritional analysis, dry matter (DM), crude protein (CP), ether extract (EE), crude fibre (CF), acid detergent fibre (ADF), neutral detergent fibre (NDF), and total ash were analyzed and the biomass yield was measured using weight balance and root and shoot length of experimental fodders. Results revealed that among three types of forages total DM was significantly (P<0.01) higher in HM and significantly (P<0.01) lower in BG. Considering total CP and NDF, it was significantly (P<0.01) higher in BG and HM than LM. The highest biomass yield and shoot length were observed in BG and the lowest biomass yield was observed in HM. Moreover, with the increment of days, all fodder sprouts DM% was constantly decreasing, however, CP% maintained the same level from day 1 to 5. Although from day 6 to 9, CP% increased moderately for HM and BG, CP% decreased slightly for LM. Therefore, farmer can use BG and HM for maximum utilization of nutrients and considering DM and CP% for LM day 6 was the best time for harvesting; however, for HM and BG, day 9 or more than 9 was the best time for harvesting. Res. Agric., Livest. Fish.8(3): 321-328, December 2021

Effect of sowing methods and weeding regimes on the yield of Sesame (Sesamum indicum L.)

The experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University to observe the effect of sowing methods and weeding regimes on the yield of sesame. The experiment comprised of two factors. Two methods of sowing i.e., broadcasting and line sowing (S2), and six weeding regimes- control (T0), weed free (T1), one hand weeding at 15 DAS (T2), two hand weedings at 15 and 30 DAS (T3), three hand weedings at 15, 30 and 45 DAS (T4) and application of Panida (Pendimethalin) herbicide (T5) at 3 DAS. The experiment was laid out in a randomized complete block design (RCBD) with three replications. Almost all the yield characters of sesame were affected significantly due to sowing methods, weeding regime and their interaction. Result showed that the l ine sowing showed better performance regarding plant height (100.40 cm), number of branches (4.983) plant–1, number of capsules plant–1 (47.82), number of seeds capsule–1 (69.66), 1000–seed weight (2.96 g), seed yield (956.90 kg ha–1), stover yield (2167.0 kg ha–1), biological yield (3123.00 kg ha–1) and harvest index (31.45%) than that of broadcasting method. Also weed free treatment had the highest plant height (101.90 cm), highest number of branches (5.00) plant-1, highest capsule (47.08) plant–1, heaviest 1000–seed weight (3.28 g), highest number of seeds capsule–1 (76.22), highest seed yield (974.30 kg ha–1), stover yield (2947.0 kg ha–1) and biological yield (3922.0 kg ha–1) as compared to other treatments while unweeded plot had least significant effect among the whole yield and yield attributes of sesame. In case of interactions, both weed free treatment and herbicidal effect with line sowing methods produced the highest plant height, number of branches and capsules plant–1, seeds capsule–1, seed yield, stover yield and biological yield (109.80 cm, 5.967, 56.57, 81.53, 1054.0 kg ha–1, 3139.0 kg ha–1 and 4193.0 kg ha–1, respectively) as compared to other interaction combinations. The results of the study reveal that the weed free treatment along with line sowing would be the proper technique for higher production of sesame. But line sowing along with application of Panida herbicide would be the best combination for obtaining higher yield of sesame since it is not feasible for the farmers to keep their field weed free throughout all the growing periods. Res. Agric., Livest. Fish.8(3): 291-299, December 2021

Biochemical and haematological profiles of black Bengal goat in Mymensingh sadar of Bangladesh

The aim of the present study was to know the normal haemato- biochemical profiles of black Bengal goats in Bangladesh. Blood samples were collected from Jugular vein of 20 Black Bengal goats which were divided into 5 groups (4 goats in each group) on the basis of age, sex, nutritional and feeding status. The whole blood was analyzed for hematology, plasma and serum samples for biochemical analysis. The results of the present study demonstrate the normal haemato-biochemical values of the Black Bengal goat breeds with their age specificity. Total RBC (15.15 ± 1.29 million/cumm), haemoglobin (7.04 ± 1.02 g/dl), total WBC (11.64 ± 1.12), differential leukocyte counts of neutrophils (70.89 ± 2.06%), lymphocyte (23.79 ± 1.69%), eosinophil 2.1%), basophil (2.3%), MCV (35.04 ± 0.47) and PCV (26.98 ± 1.49%). This study also reveals the normal bio-logical profile counts as total protein (69.47±2.31 g/L), Albumin (42.47 ± 2.31g/L), Globulin (27.32g/L), Phosphorus (3.46 ± 1.19), blood Urea (22.97 ± 0.58), Creatinine (0.645mg/dl), ALT (20.32U/L), ALP (91.59 U/L), GGT (37.46 U/L), IgG (18.91 ± 1.14 g/L), IgM ( 2.87 ± 0.18g/L), gA (.031 ± 0.12g/L) and IL-6 (1.69±0.15g/ml). The study found higher number of RBC (million/cumm), WBC (Thousands/cumm), and PCV% in the goats of age 3 years and above (18.02 ± 1.27, 14.72 ± 1.19, and 28 ± 3.2), respectively. Besides, Neutrophils% and total protein was found higher (76.289 ± 14.5%, 77.52 ± 8.43 g/L), respectively in the Black Bengal goats of 6 months to 1 year age. Present results stated some significant variation of parameters between goats of various ages; on the other hand, some shows no significant variation at all. Res. Agric., Livest. Fish.8(3): 329-337, December 2021

Value addition of tilapia (Oreochromis niloticus): Preparation of fillet and shelf-life assessment

Although tilapia is one of the most cultivated fish species in Bangladesh owing to its high growth rate, comparatively easy culture practices, higher survival rate and short culture period but low market preference marked it as a low-priced fish. Various approaches have been adopted to enhance consumer acceptability and ensure better use of tilapia, including the production of value-added products such as tilapia-prepared fillets, which attract increasing interest. Therefore, the current study was designed and carried out in order to prepare tilapia fillets and to estimate their shelf life at frozen (-18±20C) and refrigerated (4±10C) temperatures. Fillets were produced from tilapia collected from local fish markets of Sylhet city, Bangladesh with according to Good Manufacturing Practices (GMP) established by International Standards Organization (ISO) and packed in polyethylene bags. Proximate composition, total volatile base nitrogen (TVBN), peroxide value (PV), pH, total plate count (TPC) and sensory properties of fillets were analyzed to determine shelf-life. A portion of the fresh fillet sample was immediately analyzed and the remaining portions were preserved for up to 12 and 90 days at refrigerated and frozen storage temperatures, respectively. Results revealed that, at a definite interval of time, changes in the chemical, microbial and sensory attributes of tilapia fillets were found to be more pronounced in refrigerated storage conditions than fillets stored in frozen conditions. The shelf life of refrigerated and frozen tilapia fillet samples was 9 and at least 90 days, respectively, per the results of sensory, microbial and chemical evaluation. Res. Agric., Livest. Fish.8(2): 231-240, August 2021