Comparison of Whole Blood Dried on Filter Paper and Serum for Measurement of the Temporal Antibody Response to Avian Infectious Bronchitis Virus by Enzyme-Linked Immunosorbent Assay

1983 ◽  
Vol 27 (3) ◽  
pp. 813 ◽  
Author(s):  
D. P. Lana ◽  
W. W. Marquardt ◽  
D. B. Snyder
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Infectious Bronchitis Virus ◽  
Filter Paper ◽  
Whole Blood ◽  
Enzyme Linked Immunosorbent Assay ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis

scholarly journals A Peptide-Based Enzyme-Linked Immunosorbent Assay for Detecting Antibodies Against Avian Infectious Bronchitis Virus

2021 ◽  
Vol 7 ◽  
Author(s):  
Liping Yin ◽  
Qi Wu ◽  
Zhixian Lin ◽  
Kun Qian ◽  
Hongxia Shao ◽  
...  
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Infectious Bronchitis Virus ◽  
Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibody Response ◽  
Infectious Bronchitis ◽  
Conserved Sequence ◽  
Sensitivity Specificity

Infectious bronchitis virus (IBV) causes substantial loss to the poultry industry despite extensive vaccination. Assessing the antibody response is important for the development and evaluation of effective vaccines. We have developed an enzyme-linked immunosorbent assay (ELISA) for the detection of IBV-specific antibodies, using a synthetic peptide based on a conserved sequence in the IBV spike protein. This peptide-based ELISA (pELISA) specifically detects antibodies to different genotypes of IBV but not antibodies against other common chicken viruses. This assay could detect IBV-specific antibody response on as early as day 7 postinfection. In the testing with field serum samples collected from chickens administered with IBV vaccines, the sensitivity, specificity, and accuracy of pELISA were 98.30, 94.12, and 98.8%, respectively, relative to indirect immunofluorescence assay. Our data demonstrate that the pELISA is of value for the detection of IBV antibody and the evaluation of IBV vaccines.

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