Differentiation of Field Isolates of Pasteurella multocida Serotype 3,4 from Live Vaccine Strain by Genotypic Characterization

1990 ◽  
Vol 34 (2) ◽  
pp. 419 ◽  
Author(s):  
Kurt P. Snipes ◽  
Dwight C. Hirsh ◽  
Rick W. Kasten ◽  
Tim E. Carpenter ◽  
David W. Hird ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Pasteurella Multocida ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Field Isolates ◽  
Genotypic Characterization

Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae

2015 ◽  
Vol 117 ◽  
pp. 11-13 ◽  
Author(s):  
Kazumasa Shiraiwa ◽  
Yohsuke Ogawa ◽  
Masahiro Eguchi ◽  
Hirokazu Hikono ◽  
Masahiro Kusumoto ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Erysipelothrix Rhusiopathiae ◽  
Pcr Assay ◽  
Field Isolates

Differential identification of Chlamydophila abortus live vaccine strain 1B and C. abortus field isolates by PCR-RFLP

Vaccine ◽  
2010 ◽  
Vol 28 (35) ◽  
pp. 5653-5656 ◽  
Author(s):  
Karine Laroucau ◽  
Fabien Vorimore ◽  
Konrad Sachse ◽  
Evangelia Vretou ◽  
Victoria I. Siarkou ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Field Isolates ◽  
Chlamydophila Abortus ◽  
Pcr Rflp ◽  
Differential Identification

scholarly journals Effect of Various Stabilizers on Viability of Lyophilized Pasteurella multocida B:3,4 for Use as Hemorrhagic Septicemia Vaccine

2021 ◽  
Vol 25 (03) ◽  
pp. 567-574
Author(s):  
Sajid Mahmood Sajid
Keyword(s):  
Cell Viability ◽  
Vaccine Strain ◽  
Pasteurella Multocida ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Suitable Temperature ◽  
Effective Stabilizer ◽  
Skimmed Milk ◽  
Subsequent Storage ◽  
Storage Temperatures

Hemorrhagic septicemia (HS) is a devastating disease of cattle and buffaloes. The live aerosol vaccine is the best option to control HS. However, stability and viability of live vaccine is an issue. The present study was conducted to investigate the effect of three extraneous stabilizers trehalose, skimmed milk and lactalbumin on the viability of the live vaccine strain Pasteurella multocida B:3,4. The viability of the strain was evaluated using various concentrations (5, 10, 15 and 20%) of these three stabilizers. Moreover, viability of P. multocida B:3,4 was also determined at four different storage temperatures (-20, 4, 25 and 37°C). The duration of lyophilization cycle was also standardized for highest survival of cells. The data showed that trehalose and lactalbumin ensued percentage of viability as 91.89±0.08 and 80.38±2.57 respectively. Skimmed milk as stabilizer did not prove to defend cells during lyophiliztion and subsequent storage and exhibited cell viability approximately 0.47±0.009%. The study indicated that most effective stabilizer for lyophiliztion of P. multocida B:3,4 was trehalose at 15% concentration and was most suitable temperature for storage of lyophilized P. multocida B:3,4. © 2021 Friends Science Publishers

scholarly journals TolC-Dependent Modulation of Host Cell Death by the Francisella tularensis Live Vaccine Strain

2014 ◽  
Vol 82 (5) ◽  
pp. 2068-2078 ◽  
Author(s):  
Christopher R. Doyle ◽  
Ji-An Pan ◽  
Patricio Mena ◽  
Wei-Xing Zong ◽  
David G. Thanassi
Keyword(s):  
Cell Death ◽  
Immune Responses ◽  
Host Cell ◽  
Francisella Tularensis ◽  
Vaccine Strain ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Type I ◽  
Content Type ◽  
Host Cell Death

ABSTRACTFrancisella tularensisis a facultative intracellular, Gram-negative pathogen and the causative agent of tularemia. We previously identified TolC as a virulence factor of theF. tularensislive vaccine strain (LVS) and demonstrated that a ΔtolCmutant exhibits increased cytotoxicity toward host cells and elicits increased proinflammatory responses compared to those of the wild-type (WT) strain. TolC is the outer membrane channel component used by the type I secretion pathway to export toxins and other bacterial virulence factors. Here, we show that the LVS delays activation of the intrinsic apoptotic pathway in a TolC-dependent manner, both during infection of primary macrophages and during organ colonization in mice. The TolC-dependent delay in host cell death is required forF. tularensisto preserve its intracellular replicative niche. We demonstrate that TolC-mediated inhibition of apoptosis is an active process and not due to defects in the structural integrity of the ΔtolCmutant. These findings support a model wherein the immunomodulatory capacity ofF. tularensisrelies, at least in part, on TolC-secreted effectors. Finally, mice vaccinated with the ΔtolCLVS are protected from lethal challenge and clear challenge doses faster than WT-vaccinated mice, demonstrating that the altered host responses to primary infection with the ΔtolCmutant led to altered adaptive immune responses. Taken together, our data demonstrate that TolC is required for temporal modulation of host cell death during infection byF. tularensisand highlight how shifts in the magnitude and timing of host innate immune responses may lead to dramatic changes in the outcome of infection.

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