Abstract
The relationships among developmental stability, canalization and phenotypic plasticity have not been well understood. Inconsistent conclusions from different studies suggested the complexity of their associations, probably depending on specific traits, environmental contexts and plant growth stages. To address this issue, we conducted three experiments (EXP I ~ III) with several annual herbaceous species, to investigate the relationships among leaf (or cotyledon) developmental stability, canalization and plasticity and their variations with different biotic and abiotic environmental conditions and plant growth stages, with comparisons among different species at their early growth stage. We analyzed variations in mean trait value, lamina fluctuating asymmetry (FA), coefficient of variation (CV) and plasticity (RDPIs) and their correlations for lamina size (LS) of individual plants, for LS, petiole length (PL) and petiole angle (PA) of different plant layers in Abutilon theophrasti at three densities in infertile and fertile (or only fertile) soil conditions at three (or two) stages, and for cotyledon size (CS) of five species in contrasting light conditions and seeding depths. High vs. low density decreased LS (with negative RDPIs), FA indexes and CVs, either for individual plants or different layers, especially in fertile soil. Shading was more likely to increase CS (except for A. therophrasti) and FA and decrease CV; deep seeding increased CS of some species in full light, but decreased CS and FA of other species in shading (except for A. therophrasti). FA indexes more likely had positive correlations with mean value, CV and RDPIs of traits; correlations between CV and RDPIs can be positive, negative or insignificant. Correlations among the three variables were more likely positive or insignificant for traits of LS, CS and PL, but more likely negative or insignificant for PA. High density and infertile soil may favor more positive over negative correlations among variables. Results suggested higher levels of lamina FA more likely indicate higher growth rates of plants or modules. Developmental stability was more likely to have positive correlations with canalization, and negative correlations with plasticity, indicating certain common mechanisms associated with them. Environmental stresses can lead to greater phenotypic variations at different levels, facilitating cooperation between the three processes in dealing with environmental challenges.
HSP90 affects the expression of genetic variation and developmental stability in quantitative traits
