2078-P: APPL2 Deficiency Diminishes Glucose-Stimulated Insulin Secretion in Pancreatic Beta Cells

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2078-P
Author(s):  
HUIGE LIN
Keyword(s):  
Insulin Secretion ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Glucose Stimulated Insulin Secretion

Lysosomal acid lipase and lipophagy are constitutive negative regulators of glucose-stimulated insulin secretion from pancreatic beta cells

Diabetologia ◽  
2013 ◽  
Vol 57 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Gemma L. Pearson ◽  
Natalie Mellett ◽  
Kwan Yi Chu ◽  
James Cantley ◽  
Aimee Davenport ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Acid Lipase ◽  
Negative Regulators ◽  
Lysosomal Acid ◽  
Lysosomal Acid Lipase ◽  
Glucose Stimulated Insulin Secretion

scholarly journals Precise expression of Fis1 is important for glucose responsiveness of beta cells

2016 ◽  
Vol 230 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Julia Schultz ◽  
Rica Waterstradt ◽  
Tobias Kantowski ◽  
Annekatrin Rickmann ◽  
Florian Reinhardt ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Mitochondrial Dynamics ◽  
Mitochondrial Morphology ◽  
Mitochondrial Network ◽  
Primary Mouse ◽  
Glucose Stimulated Insulin Secretion ◽  
Glucose Responsiveness ◽  
Precise Expression

Mitochondrial network functionality is vital for glucose-stimulated insulin secretion in pancreatic beta cells. Altered mitochondrial dynamics in pancreatic beta cells are thought to trigger the development of type 2 diabetes mellitus. Fission protein 1 (Fis1) might be a key player in this process. Thus, the aim of this study was to investigate mitochondrial morphology in dependence of beta cell function, after knockdown and overexpression of Fis1. We demonstrate that glucose-unresponsive cells with impaired glucose-stimulated insulin secretion (INS1-832/2) showed decreased mitochondrial dynamics compared with glucose-responsive cells (INS1-832/13). Accordingly, mitochondrial morphology visualised using MitoTracker staining differed between the two cell lines. INS1-832/2 cells formed elongated and clustered mitochondria, whereas INS1-832/13 cells showed a homogenous mitochondrial network. Fis1 overexpression using lentiviral transduction significantly improved glucose-stimulated insulin secretion and mitochondrial network homogeneity in glucose-unresponsive cells. Conversely, Fis1 downregulation by shRNA, both in primary mouse beta cells and glucose-responsive INS1-832/13 cells, caused unresponsiveness and significantly greater numbers of elongated mitochondria. Overexpression of FIS1 in primary mouse beta cells indicated an upper limit at which higher FIS1 expression reduced glucose-stimulated insulin secretion. Thus, FIS1 was overexpressed stepwise up to a high concentration in RINm5F cells using the RheoSwitch system. Moderate FIS1 expression improved glucose-stimulated insulin secretion, whereas high expression resulted in loss of glucose responsiveness and in mitochondrial artificial loop structures and clustering. Our data confirm that FIS1 is a key regulator in pancreatic beta cells, because both glucose-stimulated insulin secretion and mitochondrial dynamics were clearly adapted to precise expression levels of this fission protein.

scholarly journals Metabolic concomitants in pure, pancreatic beta cells during glucose-stimulated insulin secretion.

1986 ◽  
Vol 261 (30) ◽  
pp. 14057-14061
Author(s):  
F M Matschinsky ◽  
A K Ghosh ◽  
M D Meglasson ◽  
M Prentki ◽  
V June ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Glucose Stimulated Insulin Secretion

scholarly journals Low-Level Arsenic Impairs Glucose-Stimulated Insulin Secretion in Pancreatic Beta Cells: Involvement of Cellular Adaptive Response to Oxidative Stress

2010 ◽  
Vol 118 (6) ◽  
pp. 864-870 ◽  
Author(s):  
Jingqi Fu ◽  
Courtney G. Woods ◽  
Einav Yehuda-Shnaidman ◽  
Qiang Zhang ◽  
Victoria Wong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Secretion ◽  
Beta Cells ◽  
Adaptive Response ◽  
Pancreatic Beta Cells ◽  
Low Level ◽  
Glucose Stimulated Insulin Secretion

scholarly journals Sphingosine 1-Phosphate (S1P) Regulates Glucose-stimulated Insulin Secretion in Pancreatic Beta Cells

2012 ◽  
Vol 287 (16) ◽  
pp. 13457-13464 ◽  
Author(s):  
Jamie Cantrell Stanford ◽  
Andrew J. Morris ◽  
Manjula Sunkara ◽  
Gabriel J. Popa ◽  
Kara L. Larson ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Sphingosine 1 Phosphate ◽  
Glucose Stimulated Insulin Secretion
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