scholarly journals Effect of Different Matrix Metalloproteinase Inhibitors on Microtensile Bond Strength of an Etch-and-Rinse and a Self-etching Adhesive to Dentin

2015 ◽  
Vol 40 (1) ◽  
pp. 80-86 ◽  
Author(s):  
P Zheng ◽  
M Zaruba ◽  
T Attin ◽  
A Wiegand
Keyword(s):  
Matrix Metalloproteinase ◽  
Bond Strength ◽  
Green Tea ◽  
Green Tea Extract ◽  
The Self ◽  
Mode Analysis ◽  
Microtensile Bond Strength ◽  
Mmp Inhibitors ◽  
Etch And Rinse ◽  
Tea Extract

SUMMARY Aim This study aimed to analyze the effect of different matrix metalloproteinase (MMP) inhibitors on the microtensile bond strength (microTBS) of an etch-and-rinse and a self-etching adhesive after 9 months of aging. Methods and Materials Flat human dentin surfaces were bonded either with an etch-and-rinse adhesive (Optibond FL) or a self-etching adhesive (Clearfil SE Bond). Dentin surfaces were left untreated or were pretreated with MMP inhibitors (2% chlorhexidine digluconate [CHX], 0.05% green tea extract, 1 mM ferrous sulfate, or 0.2 mM galardin) prior to application of the adhesive. Composite buildups were made incrementally. Pretreated groups were tested after 9 months of storage in artificial saliva (37°C) and compared with untreated groups, which were tested immediately (initial microTBS) and upon aging (9-month microTBS). Data were analyzed by linear mixed-model regression. Failure mode analysis was performed microscopically and statistically analyzed by repeated-measures analysis of variance (p<0.05). Results MicroTBS of the etch-and-rinse adhesive but not of the self-etching adhesive was significantly decreased by aging. For Optibond FL, pretreatment with 2% CHX, 0.05% green tea extract, and 0.2 mM galardin revealed bond strength values (MPa) similar to the initial microTBS (32.1±14.8) and significantly higher compared with the microTBS (20.3±13.6) of aged untreated dentin. No significant differences were observed between groups bonded with Clearfil SE Bond (initial microTBS: 28.3±12.4; 9-month microTBS: 25.3±11.8). Application of the MMP inhibitors decreased the number of adhesive failures compared with untreated controls after 9 months of aging, but this effect was not significant. Conclusion The MMP inhibitors prevented the decrease in microTBS upon aging of the etch-and-rinse but not of the self-etching adhesive.

scholarly journals Effect of different concentrations of green tea extract solutions on bonding durability of etch-and-rinse adhesive system to caries affected dentin

2021 ◽  
Vol 20 ◽  
pp. e210328
Author(s):  
Ricky Rolim de Moura ◽  
Fabiana Mantovani Gomes França ◽  
Cecilia Pedroso Turssi ◽  
Roberta Tarkany Basting ◽  
Flavia Lucisano Botelho do Amaral
Keyword(s):  
Bond Strength ◽  
Green Tea ◽  
In Vitro Study ◽  
Adhesive System ◽  
Green Tea Extract ◽  
Etch And Rinse ◽  
Bonding Durability ◽  
Composite Resin Restorations ◽  
Tea Extract

Aim: The in vitro study evaluated the effect of different concentrations of green tea extract solution (GT) on the bonding durability of etch-and-rinse adhesive system to caries dentin affected (CAD). Methods: Dentinal surfaces of human third molars were polished and submitted to a microbiological caries induction protocol for 14 days. After removal of the infected dentin layer, the samples were randomly divided into 4 groups (n= 10), according to the concentration of GT solution applied in CAD, after acid etching: 0.05%; 0.2%; 2% and NT (no treatment – control). After application of a etch-and-rinse adhesive system (Adper Single Bond 2, 3M ESPE), composite resin restorations were performed on the dentin. After 24 hours, the resin-dentin blocks were sectioned 1mm2 specimens, which were subjected to the microtensile test immediately or after 6 months of storage in water. Data were submitted to two-way ANOVA for randomized blocks and Tukey test (α= 5%). Results: There was no effect of double interaction (p= 0.934). The application of 0.2% GT promoted a statistically significant increase in dentin bond strength values in comparison to the condition where GT was not used (p=0.012). There was a significant decrease of bond strength after 6 months of storage, regardless of dentin pretreatment (p = 0.007). The G test identified that there was no statistical difference regarding failure mode (p= 0.326). Conclusion: The concentration of 0.2% improved the bond strength of an etch-and-rinse adhesive system to caries affected dentin, however, none of dentin pretreatments could prevent the decrease in bond strength over time.

scholarly journals Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Marine Drugs ◽  
2020 ◽  
Vol 18 (5) ◽  
pp. 263 ◽  
Author(s):  
Eugenia Baena ◽  
Sandra R Cunha ◽  
Tatjana Maravić ◽  
Allegra Comba ◽  
Federica Paganelli ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Bond Strength ◽  
Adhesive Systems ◽  
Microtensile Bond Strength ◽  
Bond Stability ◽  
Etch And Rinse ◽  
Mechanical Durability ◽  
Human Dentine ◽  
Self Etch ◽  
Metalloproteinase Activity

The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin–dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

scholarly journals Bond Strength of a Novel One Bottle Multi-mode Adhesive to Human Dentin After Six Months of Storage

2016 ◽  
Vol 10 (1) ◽  
pp. 268-277 ◽  
Author(s):  
Fernanda Borguetti Manfroi ◽  
Maurem Leitão Marcondes ◽  
Deise Caren Somacal ◽  
Gilberto Antonio Borges ◽  
Luiz Henrique Burnett Júnior ◽  
...  
Keyword(s):  
Bond Strength ◽  
The Self ◽  
Hybrid Layer ◽  
Microtensile Bond Strength ◽  
Bond Stability ◽  
Etch And Rinse ◽  
Human Dentin ◽  
And Storage ◽  
Self Etch ◽  
Multi Mode

Objective: The aim of the study was to evaluate the microtensile bond strength (µTBS) of Scotchbond Universal to dentin using the etch-and-rinse or the self-etch technique after 24 h and 6 months of storage. Materials and Methods: Flat dentin surfaces were obtained in 24 third molars. The teeth were divided into four groups: G1 – Scotchbond Universal applied in the etch-and-rinse mode; G2 – Scotchbond Universal applied in the self-etch mode; G3 – Scotchbond Multi-Purpose; G4 – Clearfil SE Bond. A block of composite was built on the adhesive area. The tooth/resin sets were cut parallel to the long axis to obtain 40 beams (~0.8 mm2) for each group. Twenty specimens were immediately submitted to the µTBS test, and the remaining 20 were stored in water for 6 months. Failures and the adhesive interface were analyzed by SEM. Results: According to two-way ANOVA, the interaction between adhesive and storage time was significant (p=0.015).The µTBS (MPa) means were the following: 24 h – G1 (39.37±10.82), G2 (31.02±13.76), G3 (35.09±14.03) and G4 (35.84±11.06); 6 months – G1 (36.99±8.78), G2 (40.58±8.07), G3 (32.44±6.07) and G4 (41.75±8.25). Most failures were mixed. Evidence of hybrid layer and numerous resin tags were noted for Scotchbond Universal applied with the etch-and-rinse mode and Scotchbond Multi-Purpose. A thinner hybrid layer and fewer resin tags were noted for Scotchbond Universal applied in the self-etch mode and Clearfil SE Bond. Conclusion: The results indicate that the µTBS for Scotchbond Universal is comparable to the gold-standard adhesives. Scotchbond Universal applied in the self-etch mode and Clearfil SE Bond revealed higher bond stability compared to the etch-and-rinse mode.

scholarly journals Microtensile Bond Strength of Etch-and-Rinse Adhesives in Different Hydroabrasion Conditionings

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Michele Tepedino ◽  
Maciej Iancu Potrubacz ◽  
Antonella Imperiale ◽  
Claudio Chimenti ◽  
Mario Capogreco ◽  
...  
Keyword(s):  
Bond Strength ◽  
Adhesive System ◽  
Mode Analysis ◽  
Microtensile Bond Strength ◽  
Cavity Preparation ◽  
Etch And Rinse ◽  
Failure Mode Analysis ◽  
Bond Strengths ◽  
Dentin Conditioning

The aim of the present study was to in vitro evaluate the effect of dentin conditioning with hydroabrasion on the microtensile bond strengths of three adhesive systems, compared to the standard etch-and-rinse technique. Sixty extracted human third molars were collected, and their midcoronal occlusal dentin was used for the microtensile bond strength test. Teeth were randomly assigned to three groups according to the adhesive system used: ExciTE F DSC, ENA Bond, and Scotchbond Universal. Specimens from each group were further divided into four subgroups: control specimens were treated with standard adhesive procedures; hydroabraded (HA) specimens were subjected to preventive hydroabrasion with three different intensity levels. After bonding procedures, composite crowns were incrementally built up. After thermocycling, specimens were subsequently sectioned into 1 × 1 mm sticks, and microtensile bond strengths were measured. Data were statistically analyzed. Failure mode analysis was performed. There were no significant differences in terms of bond strength between standard adhesion protocols and adhesion with HA preconditionings. On the other hand, the type of adhesive used had a significant effect on the tensile bond strength. Subgroups treated with hydroabrasion at higher intensity showed a slightly increased frequency of cohesive fractures. In conclusion, hydroabrasion can be used for dentin cavity preparation or finishing, since it does not seem to affect the bonding effectiveness.

scholarly journals Effects of Green Tea Application Time on Bond Strength after Enamel Bleaching

2014 ◽  
Vol 25 (5) ◽  
pp. 399-403 ◽  
Author(s):  
Andrezza Astafief Ozelin ◽  
Ricardo Danil Guiraldo ◽  
Rodrigo Varella de Carvalho ◽  
Murilo Baena Lopes ◽  
Sandrine Bittencourt Berger
Keyword(s):  
Bond Strength ◽  
Green Tea ◽  
Mode Analysis ◽  
Sodium Ascorbate ◽  
Strength Analysis ◽  
Single Bond ◽  
Third Molars ◽  
Application Time ◽  
Carbamide Peroxide ◽  
Microtensile Bond Strength

This study evaluated the effect of green tea application time on the bond strength of enamel after enamel bleaching. Enamel samples were obtained from 80 third molars and randomly divided into 7 experimental groups (G1-G7) and 1 group without treatment (G8): G1, bleached with 10% carbamide peroxide (CP); G2, CP + 10% sodium ascorbate gel (SA) for 15 min; G3, CP + SA for 30 min; G4, CP + SA for 60 min; G5, CP + 10% green tea gel (GT) for 15 min; G6, CP + GT for 30 min; G7, CP + GT for 60 min. The CP was applied onto the enamel surface for 8 h for 14 days. The SA was applied in groups 2, 3 and 4, and the GT was applied in groups 5-8 according to the above described application times. Immediately after treatment, the specimens were bonded with Adper Single Bond 2 and Filtek Z350XT. The specimens were prepared to microtensile bond strength analysis. Fracture mode analysis was performed using a stereoscopic loupe. The data were statistically analyzed by two-way analysis of variance, the Tukey's and Dunnett's tests (=5%). The means (standard deviation) were: G1, 23.3 (3.2); G2, 25.2 (3.9); G3, 26.4 (5.4); G4, 30.2 (4.5); G5, 26.6 (3.4); G6, 22.0 (5.4); G7, 31.4 (3.3); G8, 31.4 (3.2). All groups had a high percentage of adhesive failures. In conclusion, the bond strength values were higher than the value in the bleached group only when the antioxidants were applied for 60 min.

scholarly journals Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin

2016 ◽  
Vol 24 (3) ◽  
pp. 211-217 ◽  
Author(s):  
Carolina CARVALHO ◽  
Fernando Pelegrim FERNANDES ◽  
Valeria da Penha FREITAS ◽  
Fabiana Mantovani Gomes FRANÇA ◽  
Roberta Tarkany BASTING ◽  
...  
Keyword(s):  
Green Tea ◽  
Adhesive System ◽  
Green Tea Extract ◽  
Etch And Rinse ◽  
Bonding Durability ◽  
Tea Extract

The Influence of Luting Systems on the Microtensile Bond Strength of Dentin to Indirect Resin-based Composite and Ceramic Restorations

2009 ◽  
Vol 34 (3) ◽  
pp. 328-336 ◽  
Author(s):  
C. D'Arcangelo ◽  
F. De Angelis ◽  
M. D'Amario ◽  
S. Zazzeroni ◽  
C. Ciampoli ◽  
...  
Keyword(s):  
Bond Strength ◽  
Glass Ceramic ◽  
Clinical Relevance ◽  
The Self ◽  
Microtensile Bond Strength ◽  
Luting Agents ◽  
Adhesive Luting ◽  
Etch And Rinse ◽  
Ceramic Restorations ◽  
Self Etch

Clinical Relevance Compared to self-etch and self-adhesive luting systems, the two etch-and-rinse luting agents evaluated in the current study provided more reliable bonding when used to bond indirect resin-based composite restorations to sound dentin. On the contrary, the self-adhesive luting system showed the highest mean bond strength for the cementation of glass ceramic restorations.

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