Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin

Aim: The in vitro study evaluated the effect of different concentrations of green tea extract solution (GT) on the bonding durability of etch-and-rinse adhesive system to caries dentin affected (CAD). Methods: Dentinal surfaces of human third molars were polished and submitted to a microbiological caries induction protocol for 14 days. After removal of the infected dentin layer, the samples were randomly divided into 4 groups (n= 10), according to the concentration of GT solution applied in CAD, after acid etching: 0.05%; 0.2%; 2% and NT (no treatment – control). After application of a etch-and-rinse adhesive system (Adper Single Bond 2, 3M ESPE), composite resin restorations were performed on the dentin. After 24 hours, the resin-dentin blocks were sectioned 1mm2 specimens, which were subjected to the microtensile test immediately or after 6 months of storage in water. Data were submitted to two-way ANOVA for randomized blocks and Tukey test (α= 5%). Results: There was no effect of double interaction (p= 0.934). The application of 0.2% GT promoted a statistically significant increase in dentin bond strength values in comparison to the condition where GT was not used (p=0.012). There was a significant decrease of bond strength after 6 months of storage, regardless of dentin pretreatment (p = 0.007). The G test identified that there was no statistical difference regarding failure mode (p= 0.326). Conclusion: The concentration of 0.2% improved the bond strength of an etch-and-rinse adhesive system to caries affected dentin, however, none of dentin pretreatments could prevent the decrease in bond strength over time.

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Effect of Different Matrix Metalloproteinase Inhibitors on Microtensile Bond Strength of an Etch-and-Rinse and a Self-etching Adhesive to Dentin

Operative Dentistry ◽

10.2341/13-162-l ◽

2015 ◽

Vol 40 (1) ◽

pp. 80-86 ◽

Cited By ~ 30

Author(s):

P Zheng ◽

M Zaruba ◽

T Attin ◽

A Wiegand

Keyword(s):

Matrix Metalloproteinase ◽

Bond Strength ◽

Green Tea ◽

Green Tea Extract ◽

The Self ◽

Mode Analysis ◽

Microtensile Bond Strength ◽

Mmp Inhibitors ◽

Etch And Rinse ◽

Tea Extract

SUMMARY Aim This study aimed to analyze the effect of different matrix metalloproteinase (MMP) inhibitors on the microtensile bond strength (microTBS) of an etch-and-rinse and a self-etching adhesive after 9 months of aging. Methods and Materials Flat human dentin surfaces were bonded either with an etch-and-rinse adhesive (Optibond FL) or a self-etching adhesive (Clearfil SE Bond). Dentin surfaces were left untreated or were pretreated with MMP inhibitors (2% chlorhexidine digluconate [CHX], 0.05% green tea extract, 1 mM ferrous sulfate, or 0.2 mM galardin) prior to application of the adhesive. Composite buildups were made incrementally. Pretreated groups were tested after 9 months of storage in artificial saliva (37°C) and compared with untreated groups, which were tested immediately (initial microTBS) and upon aging (9-month microTBS). Data were analyzed by linear mixed-model regression. Failure mode analysis was performed microscopically and statistically analyzed by repeated-measures analysis of variance (p<0.05). Results MicroTBS of the etch-and-rinse adhesive but not of the self-etching adhesive was significantly decreased by aging. For Optibond FL, pretreatment with 2% CHX, 0.05% green tea extract, and 0.2 mM galardin revealed bond strength values (MPa) similar to the initial microTBS (32.1±14.8) and significantly higher compared with the microTBS (20.3±13.6) of aged untreated dentin. No significant differences were observed between groups bonded with Clearfil SE Bond (initial microTBS: 28.3±12.4; 9-month microTBS: 25.3±11.8). Application of the MMP inhibitors decreased the number of adhesive failures compared with untreated controls after 9 months of aging, but this effect was not significant. Conclusion The MMP inhibitors prevented the decrease in microTBS upon aging of the etch-and-rinse but not of the self-etching adhesive.

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Green Tea Extract May Prevent PIN Progression

Family Practice News ◽

10.1016/s0300-7073(05)71574-7 ◽

2005 ◽

Vol 35 (16) ◽

pp. 48

Author(s):

ROBERT FINN

Keyword(s):

Green Tea ◽

Green Tea Extract ◽

Tea Extract

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Green Tea Extract Exposure Pattern Differenzially Affects Acetaminophen-Induced Hepatotoxicity

Planta Medica ◽

10.1055/s-0031-1273663 ◽

2011 ◽

Vol 77 (05) ◽

Author(s):

A Ali ◽

X Yang ◽

Q Shi ◽

J Greenhaw ◽

WF Salminen

Keyword(s):

Green Tea ◽

Green Tea Extract ◽

Exposure Pattern ◽

Tea Extract

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Immunological and redox effects of diets enriched in glutamine and antioxidants, a green tea extract diet and a combination of these diets on several organs during endotoxemia

Aktuelle Ernährungsmedizin ◽

10.1055/s-2006-946772 ◽

2006 ◽

Vol 31 (03) ◽

Author(s):

N Unger-Manhart ◽

M Esparrech ◽

B Wessner ◽

E Roth

Keyword(s):

Green Tea ◽

Green Tea Extract ◽

Tea Extract

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Antioxidative Activity of Laver Snack Prepared with Addition of Green Tea Extract

The Korean Tea Society ◽

10.29225/jkts.2017.23.4.35 ◽

2017 ◽

Vol 23 (4) ◽

pp. 35-41

Author(s):

Jeong Hee Park ◽

Hang Yeon Jeong ◽

Jeong Yong Cho ◽

Jae Hak Moon

Keyword(s):

Green Tea ◽

Antioxidative Activity ◽

Green Tea Extract ◽

Tea Extract

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N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

Current Biochemistry ◽

10.29244/cb.6.2.3 ◽

2020 ◽

Vol 6 (2) ◽

Author(s):

Lisni Noraida Waruwu ◽

Maria Bintang ◽

Bambang Pontjo Priosoeryanto

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Green Tea ◽

Cancer Cell ◽

Breast Cancer Cells ◽

Green Tea Extract ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.105 ◽

2020 ◽

Vol 21 (1) ◽

pp. 31-35

Author(s):

Basma El-Desoky ◽

Shaimaa El-Sayed ◽

El-Said El-Said

Keyword(s):

Gene Expression ◽

Single Dose ◽

Green Tea ◽

Serum Testosterone ◽

Green Tea Extract ◽

Male Rats ◽

Controlled Study ◽

Control Group ◽

Testicular Damage ◽

Tea Extract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

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