Tumor necrosis factor-α, decoy receptor-3, ficolin-2, presepsin and heat shock protein 70 in pleural effusions

Ketuban pecah dini (KPD) berkaitan dengan peningkatan kadar Heat Shock Protein 90 (HSP 90) dan Tumor Necrosis Factor-α (TNF-α) yang muncul akibat stres oksidatif. Tujuan penelitian ini adalah membandingkan kadar HSP 90 dan TNF-α antara kehamilan preterm dengan KPD dan tanpa KPD. Penelitian ini menggunakan rancangan comparative study yang dilaksanakan di RSUD dr. Rasidin, RS Tk.III Reksodiwiryo, RS Bhayangkara, Puskesmas Lubuk Buaya dan Laboratorium Biomedik Fakultas Kedokteran Universitas Andalas dari bulan Oktober 2017 sampai Juli 2018. Jumlah sampel sebanyak 24 ibu hamil preterm dengan KPD dan 24 ibu hamil preterm tanpa KPD dengan menggunakan teknik consecutive sampling. Pemeriksaan HSP 90 dan TNF-α menggunakan metode ELISA. Uji normalitas data dengan uji Shapiro-Wilk. Analisis data komparatif menggunakan uji Mann-Whitney. Median kadar HSP 90 yaitu 11,21 ng/mL pada kehamilan preterm dengan KPD dan 9,15 ng/mL pada kehamilan preterm tanpa KPD dengan nilai p < 0,05. Median kadar TNF-α yaitu 0,21 ng/mL pada kehamilan preterm dengan KPD dan 0,17 ng/mL pada kehamilan preterm tanpa KPD dengan nilai p < 0,05. Kadar HSP 90 dan TNF-α pada kehamilan preterm dengan KPD lebih tinggi secara bermakna dibandingkan pada kehamilan preterm tanpa KPD.

Download Full-text

Tumor Necrosis Factor-α, Caspase-9 and Heat Shock Protein 70 Expressions with Leukemia Inhibitory Factor in Hydrosalpinx

10.21203/rs.3.rs-15723/v1 ◽

2020 ◽

Author(s):

Hanom Husni Syam ◽

Tono Djuwantono ◽

Jusuf S. Effendi ◽

Ponpon S. Idjradinata ◽

Tita H. Madjid ◽

...

Keyword(s):

Heat Shock ◽

Leukemia Inhibitory Factor ◽

Heat Shock Protein 70 ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Endometrial Receptivity ◽

Caspase 9 ◽

Tnf Α ◽

Factor Α ◽

Necrosis Factor

Abstract Background The leakage of hydrosalpinx fluid from the tube into the uterine cavity is likely to interfere with normal implantation. Hydrosalpinx fluids contain tumor necrosis factor-α (TNF-α), which induces caspase-9 signal transduction, leading to apoptosis. Endometrial cells inhibit apoptosis by synthesizing heat shock protein 70 (Hsp70). The TNF-α, caspase-9 and Hsp70 factors are closely related to apoptosis. In women with hydrosalpinx, the endometrial receptivity of embryonic implantation processes is low. Endometrial receptivity can be assessed by leukemia inhibitory factor (LIF). TNF-α, caspase-9 and Hsp70 expression plays an important role in endometrial receptivity disorders in women with hydrosalpinx.Materials and Methods These is an analytic observational, cross-sectional and categorical comparative study was conducted in 44 subjects with and without hydrosalpinx. The present study was performed in Dr. Hasan Sadikin Hospital, Grha Bunda-, Limijati-Maternity and Children Hospital (May-June 2017). Immunohistochemistry was performed with a cutoff based on the ROC. The Mann-Whitney analysis was performed on TNF-α, caspase-9, Hsp70 and LIF in both groups, whereas a correlation regression test was performed to observe the correlation among these protein.Results The present study used the histoscore as a tool to evaluate the expression of variable between study groups. The comparison of the histoscore for parameters between hydrosalpinx and non-hydrosalpinx subjects was TNF-α (12 vs 9; p<0,001), caspase-9 (12 vs 8,5; p<0,001), Hsp70 (6 vs 8; p<0,001), and LIF (9 vs 12; p<0,05), respectively.Conclusion The results showed a significant difference in TNF-α, caspase-9, Hsp70 and LIF (p <0.05) between hydrosalpinx and non-hydrosalpinx patients. Caspase-9 and Hsp70 are inter-connected and related to LIF as a marker in the endometrium receptivity by hydrosalpinx patients.

Download Full-text