scholarly journals Functional features of neutrophils in subclinical hypothyroidism compared to euthyroid status

2019 ◽  
Vol 3 (1) ◽  
pp. 3-9
Author(s):  
Bindu Mohan ◽  
Komaladevi Sampath Damodar
Keyword(s):  
Thyroid Hormones ◽  
Subclinical Hypothyroidism ◽  
Respiratory Burst ◽  
Phagocytic Activity ◽  
Burst Activity ◽  
Control Group ◽  
P Value ◽  
Respiratory Burst Activity ◽  
Microbicidal Activity ◽  
Functional Features

Neutrophil or polymorphonuclear leukocytes are critical to host defense. They act by migrating (chemotaxis) to site of inflammation or infection, phagocytoze the microorganisms to form phagosome. Reactive Oxygen species and hydrolytic enzymes are released (respiratory burst activity) into this phagosome to kill ingested microorganisms (microbicidal activity). Thyroid hormones are essential for proper differentiation, growth and metabolism of an organism. Increasing evidence indicates that thyroid hormones also play an active role in immunity. Subclinical hypothyroidism is associated with normal thyroid hormone levels with increased thyroid stimulating hormone level. While studies indicate elevated TSH to have some effects on immune functions, there is very little evidence regarding its effect on functional features of neutrophils. In this cross-sectional comparative study, we evaluated the phagocytic, chemotactic, candidacidal, and respiratory burst activity of neutrophils in subclinical hypothyroid and euthyroid subjects. Phagocytosis and metabolic function of the neutrophils was assessed using Nitro-blue tetrazolium test. Chemotaxis, directional movement of the PMNs was measured using the N-Formyl methionyl-leucyl-phenylalanine as chemoattractant. Phagocytic activity was evaluated by exposing leukocytes to candida suspension and counting the number of candida ingested and killed. Microbicidal activity of neutrophils was assessed by the ability of leukocytes to kill Candida albicans in both subclinical hypothyroid and euthyroid subjects. Our study demonstrated that chemotactic and mean phagocytic activity was significantly less in those with subclinical hypothyroidism as compared to euthyroid control group (p value = 0.005 and 0.045 respectively). Respiratory burst activities of both unstimulated & stimulated neutrophils were relatively reduced in subclinical hypothyroidism (p value of 0.08 and 0.07). Candidicidal activity was similar in both groups. We conclude that subclinical hypothyroid individuals having excess of thyroid stimulating hormones are associated altered functional characteristics of neutrophils.

The effect of β-hydroxy-β-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves

2013 ◽  
Vol 16 (3) ◽  
pp. 567-569 ◽  
Author(s):  
R. Wójcik ◽  
J. Małaczewska ◽  
A.K. Siwicki ◽  
J. Miciński ◽  
G. Zwierzchowski
Keyword(s):  
Respiratory Burst ◽  
Phagocytic Activity ◽  
Proliferative Response ◽  
Jugular Vein ◽  
Control Group ◽  
Blood Monocytes ◽  
Respiratory Burst Activity ◽  
Killing Activity ◽  
Group I ◽  
Experimental Group

Abstract The objective of this study was to evaluate the effect of HMB on selected indicators of immunity in calves. The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following parameters of immunity: proliferative response of LPS- and ConA-stimulated lymphocytes (MTT), respiratory burst activity (RBA) and potential killing activity (PKA) of phagocytes. The results revealed a significant increase in RBA and MTT values in calves administered HMB in comparison with the control group throughout the experiment. In the group of animals receiving HMB, an increase in PKA values was noted only on day 30.

scholarly journals The effect of Leiber Beta-S on selected immunity indicators in calves

2014 ◽  
Vol 83 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Roman Wójcik
Keyword(s):  
Respiratory Burst ◽  
Total Protein ◽  
Proliferative Response ◽  
Gamma Globulin ◽  
Burst Activity ◽  
Control Group ◽  
Respiratory Burst Activity ◽  
Killing Activity ◽  
Cellular And Humoral Immunity ◽  
Ceruloplasmin Activity

A wide variety of dietary supplements containing polysaccharides are being introduced on the market. One of them is Leiber Beta-S (β-1,3/1,6-D-glucan) whose immunostimulatory effects have not yet been fully evaluated, in particular in polygastric animals. The aim of this study was to evaluate the effect of this supplement on selected indicators of cellular and humoral immunity in calves. The experiment was performed on 14 calves aged 30 ± 2 days, divided into two equal groups of control and experimental animals. The feed administered to calves of the experimental group was supplemented with Leiber Beta-S at 50 mg/kg body weight, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled before the experiment (day 0) and on days 15, 30 and 60 to determine the immunity indicators (proliferative response of lipopolysaccharide- and concanavalin A-stimulated lymphocytes, respiratory burst activity, potential killing activity of phagocytes, gamma globulin concentrations, lysozyme activity, ceruloplasmin activity) and biochemical indicator (total protein concentrations). Diet supplemented with Leiber Beta-S stimulated the immune system of calves. Significant differences between the experimental and the control group were found in lysozyme and ceruloplasmin activity, gamma globulin concentrations, potential killing activity of phagocytes, proliferative response of lymphocytes (P < 0.001) and respiratory burst activity of phagocytes (P < 0.05). No differences were found in the serum total protein between the experimental and the control group. This study reports for the first time the effect of Leiber Beta-S (β-1,3/1,6-D-glucan) on selected biochemical and immunity indicators in calves.

scholarly journals Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation

2018 ◽  
Vol 12 (10) ◽  
pp. 894-903
Author(s):  
Marina Žekić-Stošić ◽  
Zdenko Kanački ◽  
Dragica Stojanović ◽  
Dejan Bugarski ◽  
Miodrag Lazarević ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Respiratory Burst ◽  
Transition Period ◽  
Hematological Parameters ◽  
Optimal Level ◽  
Burst Activity ◽  
Early Lactation ◽  
Health Production ◽  
Respiratory Burst Activity ◽  
Entire Study

Introduction: Hormonal and metabolic changes, as well as energy imbalance, can affect health, production and reproductive performance of dairy cows. In the present study, we evaluated phagocytosis and respiratory burst neutrophil activity during the transition period and early lactation and compared it with biochemical and hematological parameters in dairy cows. Methodology: Simmental cows (n = 21) were enrolled in the study. Whole blood samples were collected weekly from 3 weeks pre- calving until 6 weeks post calving. Basic metabolic and blood parameters were assessed by routine laboratory analyses, while neutrophil functions were analyzed by commercial test kits. Results: Optimal neutrophil response was observed pre and post calving. The highest value was recorded in the 6th week after calving (89.54 ± 7.61%) and being significantly higher (p < 0.01) as compared to values recorded at two and one week before and one week after calving. The percentage of activated neutrophils was high during the entire study period: from 70.80 ± 5.22% at the beginning of the study to 89.54 ± 7.61% at the end of the study. During the study period, production of Reactive Oxidative Species by neutrophils was positively correlated with β-hydroxybutyrat and non-esterified fatty acids values (0.454** and 0.423**, respectively) and calcium levels (0.164* and 0.212**, respectively). Conclusions: The most prominent changes in all parameters had no influence on phagocytic and respiratory burst activity of neutrophils. Neutrophil function is preserved at the optimal level during the transition period and early lactation in Simmental cows.

Ketamine inhibits polymorphonuclear leucocyte CD11b expression and respiratory burst activity in endotoxemic rats

2007 ◽  
Vol 56 (4) ◽  
pp. 149-153 ◽  
Author(s):  
Z. Q. Zhou ◽  
Y. Q. Yu ◽  
S. W. Feng ◽  
M. Yu ◽  
H. J. Liu ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Polymorphonuclear Leucocyte ◽  
Burst Activity ◽  
Respiratory Burst Activity ◽  
Cd11b Expression

Effects of fibronectin on actin organization and respiratory burst activity in neutrophils, monocytes, and macrophages

1994 ◽  
Vol 158 (2) ◽  
pp. 347-353 ◽  
Author(s):  
Kuender D. Yang ◽  
Nancy H. Augustine ◽  
Men-Fang Shaio ◽  
John F. Bohnsack ◽  
Harry R. Hill
Keyword(s):  
Respiratory Burst ◽  
Burst Activity ◽  
Respiratory Burst Activity ◽  
Actin Organization ◽  
Monocytes And Macrophages
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