scholarly journals Effect of Sucrose and Naphthalene acetic acid Concentrations on Rooting of Sugarcane In vitro

2011 ◽  
Vol 5 (2) ◽  
pp. 25-31
Author(s):  
A. A. M. Al-Salihy ◽  
Z. A. H. Al- Hussaini ◽  
Muhmmed. A. K.
Keyword(s):  
Acetic Acid ◽  
Root Length ◽  
Dry Weight ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Fresh Weight ◽  
Plant Length

hoots of two sugarcane genotypes (Co.J.64, Co.J.86) were rooted in vitro on MS medium supplemented with different concentration of sucrose (0, 30, 50, 60, 70) gm/l or NAA (0, 2.5, 5.0, 7.5, 10.0) mg/l in separate experiments. All cultures were incubated at 25±2C◦with 16 h/d light (1000 lux) for 60 days. Data of roots per plant, length, dry and fresh weights were taken after 8 weeks. Results showed that 70g/l of sucrose gave higher mean in length and fresh weight of roots reached 1.70 cm /plant, 19.80 mg/root, respectively, while 60g/l sucrose gave higher mean in number and dry weight reached 9.95 root, 4.60mg, respectively. NAA concentrations were significantly effected in root length reached 1.23 cm/plant at 5 mg/l NAA while a significant effect in root number, fresh and dry weight. The genotype Co.J.64 was the best in number of roots in sucrose experiment, while genotype Co.J.86 surpassed in number of root, fresh and dry weight in NAA experiment.

scholarly journals Bulblet regeneration from ex vitro root explant in lily hybrids

2008 ◽  
Vol 35 (No. 3) ◽  
pp. 107-112 ◽  
Author(s):  
R. Kapoor ◽  
S. Kumar ◽  
K. Kanwar J
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Middle Zone ◽  
Ex Vitro ◽  
Fresh Weight ◽  
Root Segments ◽  
Root Explant

The influence of growth regulators on <i>in vitro</i> bulblet formation from <i>ex vitro</i> roots was studied in asiatic and oriental hybrids of <i>Lilium</i> The root segments (3–4 mm) isolated from the middle zone of 2–3 cm <i>ex vitro</i> root were cultured on Murashige and Skoog (MS) medium containing 1 or 1.5 mg/dm<sup>3</sup> naphthalene acetic acid (NAA) and/or benzyladenine (BA). Bulblets were not produced in the presence of NAA and BA alone. A significant increase in the per cent explants producing bulblets was observed with 1.5 mg/dm<sup>3</sup> NAA and 1 mg/dm<sup>3</sup> BA. Maximum number of bulblets and average fresh weight per bulblet was observed with 2 mg/dm<sup>3</sup> NAA and 1.5 mg/dm<sup>3</sup> BA after 90 days of culture. No differences were found among cultivars in bulblet regeneration of explant or bulblet number although more weighty bulblets occurred in cv. Apeldoorn. About 82% bulblet survival was recorded in coco peat after 30 days of transfer to pots.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals INFLUNCE OF AUXIN AND POLYAMINEES ON ROOTING OF SHOOTS OF CITRUS VOLKAMERIANA ROOTSTOCK IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Al- Khazali & Hamad
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Tissue Culture ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
College Of Agriculture ◽  
Indole Butyric Acid ◽  
Citrus Volkameriana

This  research  was  conducted  in  the  plant  tissue  culture  Lab. College  of Agriculture / University  of  Baghdad  from  February to  October  2015. The aim  of  the  study  was  investigating  the  influence  of  combinations  of Indole  butyric  acid (IBA) ,  Naphthalene  acetic  acid (NAA) and  polyamine Spermidine (Spd.) on rooting of shoots of  citrus volkameriana rootstock cultured  on 1\2  MS medium. The Results indicated that 1/2 MS medium supplemented  with 1.0 mg L-1 (IBA)  gave the highest  percentage  of  rooting  (67 %) which differed significantly  from the MS medium with free auxin IBA  that gave (22%) while  the  MS medium  supplemented with 0.5 mg L-1  spermidine  gave the highest percentage of rooting (63%) that was not significantly different  than other concentrations of Spd . MS medium supplemented with 1.0 mg L-1 IBA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (83%) and the highest root number / shoot (3.17) and highest length of root (3.15 cm) while the MS medium with free auxin IBA and spd. did not give percentage of rooting (0%) for citrus volkameriana rootstock plantlets . The MS medium supplemented with 1.0 mg L-1 NAA gave the highest percentage of rooting (56%) which differed significantly from MS medium with free auxin NAA that gave (22%)  while MS medium supplemented with 0.5mg L-1 spd. gave the highest percentage of rooting (50%) that was not significantly different from other concentration of Spd . MS medium supplemented with 1.0 mg L-1 NAA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (68%) and the highest root number /shoot (2.5) and highest length of root (2.65 cm) while the MS medium with free auxin NAA and Spd. did not gave percentage of rooting (0%) .

scholarly journals INFLUNCE OF GROWTH REGULATORS ON CALLUS INDUCTION OF CITRUS VOLKAMERIANA IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Al- Khazali & Hamad
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Dry Weight ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
College Of Agriculture ◽  
Citrus Volkameriana ◽  
Dichlorophenoxy Acetic Acid

This  research  was  conducted  in  the  plant  tissue  culture  Lab. College  of Agriculture / University  of  Baghdad  from  February to  October  2015. The aim  of  the  study  was  investigating  the  influences  of  combinations  of  Naphthalene  acetic  acid (NAA) , Thidiazuron (TDZ) Spermidine  (Spd. ) and 2,4-Dichlorophenoxy  acetic  acid (2,4-D) , Benzyl  adenine (BA) on callus  induction  and  adventitous  shoot  regeneration  originated  from  cotyledon  of  Citrus volkameriana  seeds. Seeds  were  disinfested  with 0.1 % of  HgCl2  for 15 minutes. The MS  medium  supplemented  with  (0.0,1.5 , 3.0 ) mg L-1  NAA in combination with (0.0, 0.05, 0.1) mg L-1  TDZ and (0.0, 0.5 ,1.0) mg L-1 Spd. and MS medium supplemented with (0.0, 1.5 , 3.0) mg L-1  2,4-D in combination with (0.0 ,1.0 , 2.0 )  mg L-1  BA and (0.0 ,0.5 , 1.0) mg L-1  Spd. the  interaction between 1.5 mg L-1  NAA and (0.05 , 0.1)  mg L-1  TDZ and the interaction between 3.0 mg L-1  NAA and (0.05 ,0.1) mg L-1  TDZ with all concentrations of Spd.   gave  the  highest  percentage of  callus  induction  100 % . While  the  MS  medium  supplemented  with  3 mg L-1 of  2,4-D in  combination  with  all  concentrations  of  BA  and  spd.  gave  the  highest  percentage 100 % of  callus induction. Results showed that  MS medium supplemented  with 1.5 mg L-1  NAA in combination  with  0.1 mg L-1 TDZ and  1.0 mg L-1 spd.  gave  the  highest  values  of  fresh  and  dry  weight  of  callus  (668.8, 44.59 ) mg  respectively . While  the  MS  medium  supplemented  with  3 mg L-1 2,4-D  in combination with 1.0 mg  L-1  spd.  And 0.0 mg L-1 BA gave  the  highest  values  of  fresh  and  dry  weight  of  callus  (709.2 , 47.28 ) mg  respectively.

scholarly journals INFLUENCE OF LIGHT SPECTRUM AND NAPHTHALENE ACETIC ACID ON ROOTING OF SHOOTS FOR TWO VARIETIES OF GERBERA JAMESONII IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
AL-Hamidawi & Hamad
Keyword(s):  
Acetic Acid ◽  
White Light ◽  
Root Length ◽  
Red Light ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Gerbera Jamesonii ◽  
Light Spectrum

This research was conducted at the plant tissue culture Lab. College of Agriclture, University of Baghdad from February to October 2015. The aim of the study was investigate the  influence of light quality and Naphthalene  Acetic Acid(NAA) on some properties of roots for two varieties Martinque and Yanara shoots of Gerbera jamesonii, cultured on MS medium supplemented with 0.3 mg.L -1 Spermidine. The results indicated that 1/2 MS medium with free auxin NAA gave the highest percentage of rooting (75 %) Which differed significantly from the other concentrations of NAA. White light gave the hightest percentage of rooting (89 %), that was not significantly different from the red  light (78%) . While the treatment of Martinque variety under White light gave the highest percentage of rooting (100%), that was not significantly different from Yanara variety that gave (78%). MS medium with free auxin NAA gave the highest number of roots (7.5 % root/shoot ) that was significantly different on all the other concentrations of NAA. MS medium with free auxin NAA under White light of roots (6.6 root/shoot) at Martinque variety . While MS medium with free auxin NAA under blue light gave the highest number of roots (10.10 roots/shoot) that was not different significantly from red light it gave (8.9 root/shoot) Martinque variety gave the highest rate of root length ( 1.62 cm) Which not differend  significantly from Yanara variety it gave (1.49 cm) While The treatment of Martinque variety under White light with NAA- free medium gave the highest rate of root length (3.40 cm) and was significantly different on all the other interactions

scholarly journals Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base

2013 ◽  
Vol 21 (2) ◽  
pp. 23-30 ◽  
Author(s):  
Fardin Nasri ◽  
Seyed Najmedin Mortazavi ◽  
Nasser Ghaderi ◽  
Taimoor Javadi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Shoots ◽  
Shoot Length ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Leaf Base

ABSTRACT In the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) concentrations (0, 0.5, 1, 1.5 and 2 mg·1-1) and three indole-3-butyric acid (IBA) concentrations (0, 0.1 and 0.2 mg·1-1). The cultures were incubated at 21 ± 2 °C under photoperiod 16/8. After three subculturings (3 weeks-long each) the number of rhizome, shoots, buds, leaves, and roots, length of shoots and roots were recorded. Adventitious shoots formed directly on the leaf bases without callus intervention. Their number was affected by BAP concentrations. The highest shoots number, six per explants, was obtained at 1 mg·1-1 BAP and 0.1 mg·1-1 IBA. The shoot length decreased with the increasing concentration of BAP. The highest root number (2.7) was formed on shoots cultured on the MS medium containing 0.5 mg·1-1 α- naphthalene acetic acid, and the highest rhizome number (2.2) was formed on the medium with 0.5 mg·1-1 BAP. In vitro rooted plantlets were able to survive and acclimatize in the greenhouse.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

2020 ◽  
Author(s):  
Nurşen Çördük ◽  
Cüneyt Aki
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Northwestern Turkey ◽  
Helen Of Troy ◽  
Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

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