Isolation and Amplification of Touch DNA from Portable Computer

2021 ◽  
Vol 15 (1) ◽  
pp. 5-10
Author(s):  
Miriam Jasim Shehab ◽  
Majeed Arsheed Sabbah ◽  
Dhuha Salim Namaa ◽  
Sura Nabeel
Keyword(s):  
Gel Electrophoresis ◽  
Forensic Analysis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Forensic Dna ◽  
Touch Dna ◽  
Portable Computer ◽  
Suitable Amount ◽  
Crime Scenes

    Analysis of touched DNA from crime scenes is fundamental in forensic DNA laboratories. Many factors affect the recovery of DNA from touched surfaces and then affect the quality of the final results.  The aim of this work is studying the possibility of recovery suitable amount of DNA from ltouched portable computer. The computer was cleaned with 10% Bleach then touch and DNA collected for extraction by Organic method and two STR regions D5S818 (115-163bp) and FGA (308-464bp) were amplified. The results showed that it is possible to isolate a proper amount of DNA from touched portable computer where it was amplified and then analyzed by Agarose gel electrophoresis. The conclusion is that portable computer is suitable source for forensic analysis.

Nucleic Aacid Extraction from Biomining Microorganisms

2009 ◽  
Vol 71-73 ◽  
pp. 159-162 ◽  
Author(s):  
Carla M. Zammit ◽  
L.A. Mutch ◽  
Helen R. Watling ◽  
Elizabeth L.J. Watkin
Keyword(s):  
Nucleic Acid ◽  
Gel Electrophoresis ◽  
Extraction Method ◽  
Quantitative Method ◽  
Agarose Gel ◽  
Mineral Surfaces ◽  
Agarose Gel Electrophoresis ◽  
Quantitative Real Time Pcr ◽  
Cellular Lysis

Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on the quantity of NA extracted from each strain. The quantity and quality of extracted NA was highly dependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms.

scholarly journals Optimasi Isolasi DNA Cabai (Capsicum annuum L.) Berdasar Perbedaan Kualitas dan Kuantitas Daun serta Teknik Penggerusan

2013 ◽  
Vol 15 (1) ◽  
pp. 14
Author(s):  
Rejeki Siti Ferniah ◽  
Sri Pujiyanto
Keyword(s):  
Capsicum Annuum ◽  
Gel Electrophoresis ◽  
Dna Isolation ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Fresh Leaf ◽  
Grinding Technique ◽  
Commercial Kit ◽  
Better Than

Complete genome of chili has not been reported.The first step to study the genome is DNA isolation, so it is necessary to optimize the protocol to get an optimum DNA. This research aimed to optimize chili DNA isolation by variate the quantity and quality of chili leaves as row material and variate the grinding technique. DNA isolation was done using commercial kit without liquid nitrogen, and analyzed by agarose gel electrophoresis. The results showed that frozen chili leaf yields more DNA than fresh leaf, 0,1 g of leaf got optimum DNA, and grinding in mortar was better than in microtube.   Key words: DNA,  isolation, Capsicum annuum

scholarly journals TECHNICAL SHEET OF COMPARATIVE STUDY OF TWO TECHNIQUES CONTROL OF DNA FROM YEAST ISOLATES PRODUCING PECTINASE

2020 ◽  
Vol 8 (9) ◽  
pp. 979-983
Author(s):  
Coulibaly Wahauwouele Hermann ◽  
◽  
Bouatenin Koffi Maizan Jean-Paul ◽  
Kouame Kohi Alfred ◽  
Amoikon Tiemele Laurent Simon ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Biology ◽  
Spectrophotometric Method ◽  
Gel Electrophoresis ◽  
Extraction Method ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction (PCR) is a widely used technique in the field of molecular biology to rapidly make very large number of copies of a specific DNA sample for detailed studies. The success of the technique however is dependent on the on the quality, i.e purity of the extracted DNA specimen. The aim of this study was to evaluate the quality of extracted DNA from pectinase producing yeast to determine the suitability of the extraction method to produce pure extract without non-inhibiting substances.In this study, DNA extracts from six (06) isolates of pectinase-producing yeasts were quantitatively and qualitatively analyzed using NanoDrop spectrophotometry and agarose gel electrophoresis methods. These analyses showed that the concentration of DNA extracts from the isolates evaluated by the NanoDrop spectrophotometric method ranged from 403.8 to 1082.4 ng/µL and the purity index A 260/280 was between 2.03 and 2.11. In sum, agarose gel electrophoresis showed that the intensity of the DNA bands was irregular and not necessarily in line with the data provided by the NanoDrop spectrophotometry.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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