scholarly journals PENGARUH IMBANGAN RUMPUT LAPANGAN DENGAN LIMBAH KOL (Brassica oleracea var capitata L.) TERHADAP TOTAL BAKTERI DAN PROTOZOA PADA CAIRAN RUMEN DOMBA (IN VITRO)

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Pungky Marantika
Keyword(s):  
Brassica Oleracea

Budidaya sayuran kol menyisakan limbah yang dapat digunakan sebagai pakan sumber hijauan. Penelitian bertujuan untuk mengevaluasi pengunaan limbah kol untuk mengantikan rumput lapangan terhadap populasi bakteri dan protozoa rumen. Penelitian dilaksanakan secara eksperimental dengan menggunakan rancangan acak lengkap. Perlakuan terdiri 5 macam ransum percobaan yaitu perbandingan rumput lapangan dengan limbah kol 100:0 (T1), 75:25 (T2, 50:50 (T3), 25:75 (T4), dan 0:100 (T5), masing-masing perlakuan diulang 4 kali. Data yang terkumpul sebelum dianalysis dengan sidik ragam, dilakukan transformasi logaritma dan jika terjadi perbedaan yang nyata dianalisis dengan uji Dunnets. Sebagai ransum kontrol adalah T1 yaitu 100% rumput lapangan. Hasil menunjukkan bahwa perlakuan berpengaruh terhadap populasi bakteri, namun tidak pada populasi protozoa. Perlakuan T2 dan T5 berbeda nyata (P<0,05) dibandingan ransum control (T1). Populasi protozoa tertinggi diperoleh pada perlakuan T5 yaitu 14,26 x 1011 sel/mL, sedangan populasi protozoa untuk semua perlakuan pada kisaran 5,25-9,75 x 105 sel/mL. Kesimpulan, penggunaan 100% limbah kol sebagai hijauan menghasilkan populasi bakteri yang maksimal.

Cytotoxic Effect of Brassica oleracea Extract on two Tumor Cell Lines in vitro

2013 ◽  
Vol 16 (1) ◽  
pp. 137-142
Author(s):  
Farooq I. Mohammed ◽  
◽  
Farah T. Abdullah ◽  
Shaimaa Y. Abdulfttah ◽  
◽  
...  
Keyword(s):  
Tumor Cell ◽  
Brassica Oleracea ◽  
Cell Lines ◽  
Cytotoxic Effect ◽  
Tumor Cell Lines

scholarly journals PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

2018 ◽  
Vol 14 (1) ◽  
pp. 439
Author(s):  
Alfrida ., Maninggolang ◽  
Jeany Sh. Polii-Mandang ◽  
Wenny ., Tilaar
Keyword(s):  
Brassica Oleracea ◽  
Coconut Water ◽  
Shoot Bud ◽  
Randomized Design ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Brassica Oleracea L ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

scholarly journals In Vitro Effects of Different Combinations of Phytohormones on Callus Induction from Different Explants of Cabbage Brassica Oleracea Var. Capitata L. Seedlings

2021 ◽  
pp. 3476-3486
Author(s):  
Alaa. M. Hasan ◽  
Ekhlas. A.J. ElKaaby ◽  
Rakad. M.Kh. AL-Jumaily
Keyword(s):  
Brassica Oleracea ◽  
Callus Induction ◽  
Basal Medium ◽  
Culture Conditions ◽  
Control Treatment ◽  
Fresh Weight ◽  
Superior Result ◽  
Significant Difference ◽  
In Vitro Effects

    The leading purpose of this work is the development of efficient culture conditions to induce calli from cabbage (Brassica oleracea var. capitata L.) under in vitro conditions. The mature seeds were surface sterilized with combinations of different concentrations of ethanol and NaOCl in different time durations and  were germinated on MS basal medium. The results revealed that the best sterilization method of cabbage seeds was by using 70% ethanol for one minute, followed by 15 min in 2% (NaOCl). Seedlings were used as donor sources for hypocotyls, cotyledon leaves, true leaves, and shoot tip explants. These explants were cultured on different combinations of cytokinins (TDZ, BAP, Ad) and auxins (IAA, NAA, 2, 4-D) then implanted in Murashige and Skoog (MS) media. 4 weeks after culturing, a significant difference was found among the explants in response to plant hormones. The maximum percentage of callus induction (100%) was using the combinations of 1 BAP + 1 2, 4-D, 1 BAP + 1 NAA, and 1 BAP + 2 2,4-D mg. l-1. In addition, explants responses varied and the hypocotyls showed a superior result (85.71 %) as compared to other explants. For callus fresh weight, the combination of 0.22 TDZ + 79.9 Ad mg. l-1    had a significant effect, causing the highest fresh weight (0.2745g), while control treatment gave the lowest mean of 0.0066 g. Data showed that cotyledon explants were significantly superior in giving highest callus fresh weight with the mean of 0.1723 g. On the other hand, hypocotyl explants gave the lowest mean, reaching 0.1542 g.

scholarly journals The Influence of pH on Microspore Embryogenesis of White Cabbage (Brassica oleracea L.)

2013 ◽  
Vol 5 (4) ◽  
pp. 485-489 ◽  
Author(s):  
Tina Oana CRISTEA
Keyword(s):  
Brassica Oleracea ◽  
Microspore Culture ◽  
Microspore Embryogenesis ◽  
Critical Factor ◽  
Cellular Level ◽  
Strong Base ◽  
White Cabbage ◽  
In Vitro Microspore Culture ◽  
Influence Of Ph

In vitro microspore culture is one of the top techniques utilised now-a-days for the obtaining of double haploid plants in many plant species, including Brassica. The pH of the medium is a critical factor for the success of In vitro microspore culture as it influences the invertase enzyme activity, translated at cellular level through an acceleration or reduction of sucrose cleavage. The results published until now shows rather contradictory findings, as the response of microspores have been proved to be highly depending on genotypes, most of them being focused on Brassica napus. Thus, in the present study, the effect of different NLN liquid medium pH, ranging between 5.0 to 7.0 were tested in order to establish the most suitable pH for the expression of embryogenic competences of microspores cultivated on medium In vitro and ultimately for the obtaining of microspore-derived embryos. Among the 11 values of pH tested, the best results were obtained on variants with pH 5.8 and 6.0, both in what concern the maintaining of microspores viability and the number of microspore-derived embryos. The findings of the present study provide a strong base for the establishment of an efficient protocol for the In vitro culture of microspore at Brassica oleracea L. genotypes with Romanian origin.

THE EFFECTS OF PARAQUAT ON CABBAGE (BRASSICA OLERACEA VAR. CAPITA L.) CULTURED IN VITRO

1997 ◽  
pp. 589-594
Author(s):  
T. Sretenovic-Rajicic ◽  
D. Vinterhalter ◽  
B. Vinterhalter
Keyword(s):  
Brassica Oleracea

scholarly journals In vitro inhibitory potentials of aqueous and ethanol extracts of Hyptis suaveolens on fungi associated with postharvest spoilage of Brassica oleracea

2018 ◽  
Vol 17 (31) ◽  
pp. 949-958
Author(s):  
R. Agbawodike Chidiebere ◽  
A. Sobowale Ayodele ◽  
S. Gbolagade Jonathan ◽  
Oscar N. Aguzie Ifeanyi
Keyword(s):  
Brassica Oleracea ◽  
Hyptis Suaveolens ◽  
Ethanol Extracts
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