scholarly journals PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

2018 ◽  
Vol 14 (1) ◽  
pp. 439
Author(s):  
Alfrida ., Maninggolang ◽  
Jeany Sh. Polii-Mandang ◽  
Wenny ., Tilaar
Keyword(s):  
Brassica Oleracea ◽  
Coconut Water ◽  
Shoot Bud ◽  
Randomized Design ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Brassica Oleracea L ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

scholarly journals Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

2019 ◽  
Vol 67 (1) ◽  
pp. 9-18
Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Indole 3 Acetic Acid ◽  
Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

scholarly journals The shoot explant types effect of eleven stevia (Stevia rebaudiana Bertoni)

Jurnal Agro ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 142-152
Author(s):  
Suseno Amien ◽  
Arini Zahra Azhari ◽  
Citra Bakti ◽  
Haris Maulana
Keyword(s):  
Stevia Rebaudiana ◽  
Shoot Tip ◽  
Shoot Height ◽  
Stevia Rebaudiana Bertoni ◽  
Randomized Design ◽  
Two Factors ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Number Of Shoots

Explants play an important role in thepropagation system of stevia (Stevia rebaudiana Bertoni). A Completely Randomized Design (CRD) was used in this experiment with factorial pattern consisting of two factors, namely three types of explants (shoot tip, first node, and second node) and eleven accessions of stevia namely a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). The results showed that shoot tip explant was the best explant than first node and second node for the number of shoots (18.11 shoots), number of leaves (93.49 leaves) and wet weight (3.56 grams). The best accession of shoot height was a10(SGR 7.5) (19.95 cm), the highest number of shoots wasa7(SBG 10) (21.87 shoots), the highest number of leaves wasa7(SBG 10) (138.00 leaves), the heaviest wet weight wasa7(SBG 10) (3.56 grams), the highest leaf chlorophyll content was a10 (SGR 7.5) (0.63 µg mL-1). Accessions with the fastest root initiation time at the rooting stage was a11 (TR 3.5) (4.00 DAC), the highest number of roots wasa10 (TR 3.5) (27.11 roots), the best root length wasa2(Garut) (4.51 cm). Information on the best explant types and stevia accessions in the in-vitro multiplication stage can be used as the basis for stevia breeding programs in Indonesia.AbstractEksplan berperan penting dalam sistem perbanyakan Stevia (Stevia rebaudiana Bertoni). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan pola faktorial yang terdiri dari dua faktor yaitu tiga jenis eksplan (ujung pucuk, ruas pertama, dan ruas kedua) dan sebelas aksesi stevia yaitu a1 (Bogor), a2 (Garut), a3 (Canada), a4 (Tawangmangu), a5 (STG1), a6 (SBG 4), a7 (SBG 10), a8 (SGB 2), a9 (BR5), a10 (SGR 7.5), a11 (TR 3.5). Hasil penelitian menunjukkan bahwa eksplan ujung pucuk merupakan eksplan terbaik dibandingkan buku pertama dan kedua untuk jumlah tunas (18,11 tunas), jumlah daun (93,49 daun) dan berat basah (3,56 gram). Aksesi terbaik pada tinggi pucuk adalah a10(SGR 7.5) (19,95 cm), jumlah pucuk tertinggi a7 (SBG 10) (21,87 pucuk), jumlah daun terbanyak a7 (SBG 10) (138.00 helai daun), bobot basah terberat a7 (SBG 10) (3,56 gram), kandungan klorofil daun tertinggi adalah GR 7,5 (0,63 µg mL-1). Aksesi dengan waktu inisiasi akar tercepat pada tahap perakaran adalah TR 3,5 (4,00 HST), jumlah akar terbanyak adalah TR 3,5 (27,11 akar), panjang akar terbaik adalah a2(Garut) (4,51 cm). Informasi jenis eksplan dan aksesi stevia terbaik pada tahap multiplikasi in-vitro dapat dijadikan dasar program pemuliaan stevia di Indonesia.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

scholarly journals THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina
Keyword(s):  
Shoot Growth ◽  
Good Condition ◽  
Kiwi Fruit ◽  
Physiological Maturity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Time Required ◽  
Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

scholarly journals Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

2021 ◽  
Vol 922 (1) ◽  
pp. 012067
Author(s):  
Sophia ◽  
M Hayati ◽  
E Kesumawati
Keyword(s):  
Callus Formation ◽  
Formation Rate ◽  
Water Concentration ◽  
Shoot Formation ◽  
Coconut Water ◽  
Contamination Rate ◽  
Two Factors ◽  
Number Of Leaves ◽  
Benzyl Amino Purine

Abstract In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.

scholarly journals Propagation of Dendrobium aggregatum by green capsule culture

Lankesteriana ◽  
2013 ◽  
Author(s):  
S. Vijayakumar ◽  
G. Rajalkshmi ◽  
K. Kalimuthu
Keyword(s):  
Root Formation ◽  
Coconut Water ◽  
Immature Embryos ◽  
Ms Medium ◽  
Medium Supplement ◽  
In Vitro Shoots ◽  
Immature Seeds ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.

scholarly journals PENGARUH LAMA PERENDAMAN AIR KELAPA TERHADAP PERTUMBUHAN DAN HASIL TANAMAN BAWANG MERAH (Allium ascalonicum. L) PADA BERBAGAI MACAM MEDIA

2019 ◽  
Vol 13 (1) ◽  
pp. 33-44
Author(s):  
Fendi Ramdhani
Keyword(s):  
Water Immersion ◽  
Block Design ◽  
Dry Weight ◽  
Coconut Water ◽  
Growth And Yield ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Number Of Shoots

Demand for shallots continues to increase due to increasing population and consumption of mera onions. This research was carried out in jingglong village, Sutojayan sub-district, Blitar district in April - June 2018. This research was conducted using factorial randomized block design (RBD) with 2 factors, the first factor was immersion in coconut water (k) which consisted of 3 levels namely immersion for 1 hour (k1) soaking for 2 hours (k2) soaking for 3 hours (k3). The second factor is various media (m) consisting of 3 levels: soil 1: fertilizer 1: husk ash 1 (m1), soil 2: fertilizer 1: husk ash 1 (m2), soil 1: fertilizer 2: husk ash 1 ( m3) The variables observed included plant height, number of shoots, number of leaves, weight of tuber stover, wet weight of tubers, dry weight of tubers. Data were analyzed using analysis of variance (ANOVA) level of 5% and further testing with Duncan's Multiple Range Test (DMRT). There is no real interaction between coconut water immersion and various planting media on the growth and yield of shallots. The treatment of coconut water immersion (k) significantly affected the height of shallot plants at the age of 14 days and 21 days, and affected the number of shoots and leaves at all ages of observation. The treatment of various planting media showed a significant difference at the age of 14 days to 21 days. The treatment of various planting media had a significant effect on the weight of stover and the wet weight of onion tubers with the highest yield (m1) of 122.03 gr, while in the wet weight the highest yield was on media (m1) with a yield of 80.51 gr.

scholarly journals In Vitro Response of Protocorm Grammatophyllum stapeliiflorum (Teijsm. & Binn.) J.J.Sm. Orchid in Growth on Several Media Composition

2019 ◽  
Vol 7 (2) ◽  
pp. 118
Author(s):  
Ranny Wirmasari ◽  
Mayta Novaliza Isda
Keyword(s):  
Growth And Development ◽  
Coconut Water ◽  
Media Composition ◽  
Randomized Design ◽  
In Vitro Response ◽  
Completely Randomized Design ◽  
Original Habitat ◽  
Test Treatment ◽  
Number Of Shoots

Family Orchidaceae has about 800 genera which are already difficult to find and almost extinct, including the orchid genus Grammatophyllum. A species of rare orchids, Grammatophyllum stapeliiflorum, is hard to find in original habitat. The addition of Growmore, BAP and 15% coconut water is expected to increase the growth and development of the G. stapeliiflorum orchid protocorms. This study use a Completely Randomized Design, consisting of 6 treatments (control, 1 and 3 mg/l BAP, 1 and 3 g/l Growmore, and 150 ml/l coconut water) in MS media with 5 replicates with observation for 4 weeks after planting. The results showed did not differ markedly in the number of protocorms, number of shoots and number of browning protocorms, but differ markedly in the parameter protocorm color based on DMRT test. Treatment of 3 mg/l BAP gives the best results on the number of shoots 2,60 protocorms and number of browning protocormss at the least amount 0,80 protocorms. The number of protocorms most widely on the treatment of 1 g/l Growmore 14,40 protocorms. Protocorms color is best found in 3 g/l Growmore with green color. This research managed to multiply the number of protocorms and induces protocorms shoots from Grammatophyllum stapeliiflorum orchid.

Sign in / Sign up

Export Citation Format

Share Document