scholarly journals NILAI DIAGNOSTIK ANTI DENGUE IgA DAN NS1, SERTA IgM/IgG DI INFEKSI VIRUS DENGUE (The Diagnostic Value of Anti Dengue IgA and Anti Dengue IgM/IgG in Dengue Virus Infection)

2018 ◽  
Vol 21 (1) ◽  
pp. 82
Author(s):  
Resna Resna ◽  
Aryati Aryati ◽  
Puspa Wardhani ◽  
Erwin Triyono
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Likelihood Ratio ◽  
Predictive Value ◽  
Secondary Infection ◽  
Clinical Manifestations ◽  
Negative Likelihood Ratio ◽  
Diagnostic Value ◽  
Asia Pacific ◽  
Dengue Virus Infection

The clinical manifestations of dengue virus infection are varied and thus a specific diagnostic examination is required. Usually antidengueIgM is often used, but the presence in the circulation is 3−8 months long. NS1 is sensitive in the detection of primary infection,whereas IgG is more better used in secondary infection. The examination of anti-dengue IgA as a new marker is estimated to be ableto detect the acute primary and secondary infection, however the diagnostic value of anti-dengue IgA is not much well known for theIndonesian population. This study was done at the Tropical Infectious Disease Ward of Dr. Soetomo Hospital, Surabaya during February– April 2013. The samples consisted of 37 sera from patients infected by dengue virus and 37 sera from those non one (dengue virusinfection patients). The NS1 serum, anti-dengue IgM and anti dengue IgG were examined by ELISA and anti-dengue IgA was examined byan indirect immunochromatography method using Assure@ Dengue IgA Rapid Test (MP Biomedicals Asia Pacific Pte Ltd). The diagnosticvalue was analyzed by 2x2 table with a confidence interval of (CI) 95%. The used gold standards were from the 1997th WHO criteriaand one of the positive dengue serological tests by ELISA (NS1/anti dengue IgM/anti dengue IgG). AUC and anti-dengue IgA cut-off weredetermined by ROC curve. The Diagnostic value of anti-dengue IgA showed a sensitivity and specificity of 83.8% (67.3 to 93.2) and 81.1%(64.3 to 91.4). A positive predictive value of 81.6% (65.1 to 91.7) and a negative predictive value of 83.3% (66.5 to 93.0) was found. Thepositive likelihood ratio was 4.4 times (2.2 to 8.8) and negative likelihood ratio of only 0.2 times (0.09 to 0.42). The best cut off valueof 0.2 was shown by the area under the curve of 83.5%. Based on this study, the diagnostic value of anti-dengue IgA had a good validityfor the diagnosis of dengue virus infection.

scholarly journals Manifestation of thrombocytopenia in dengue-2-virus-infected mice

2000 ◽  
Vol 81 (9) ◽  
pp. 2177-2182 ◽  
Author(s):  
Kao-Jean Huang ◽  
Shu-Yi J. Li ◽  
Shiour-Ching Chen ◽  
Hsiao-Sheng Liu ◽  
Yee-Shin Lin ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Clinical Manifestations ◽  
Dengue Shock Syndrome ◽  
Dengue Virus Infection ◽  
Platelet Antibody

Dengue virus infection causes dengue fever, dengue haemorrhagic fever and dengue shock syndrome. No animal model is available that mimics these clinical manifestations. In this study, the establishment is reported of a murine model for dengue virus infection that resembles the thrombocytopenia manifestation. Dengue-2 virus (dengue virus type 2) can infect murine cells either in vitro (primary cell culture) or in vivo. Viraemia detected by RT–PCR was found transiently at 2 days after intravenous injection of dengue-2 virus. Transient thrombocytopenia developed at 10–13 days after primary or secondary infection. Anti-platelet antibody was generated after dengue-2 virus infection. There was strain variation in dengue-2 virus infection; the A/J strain was more sensitive than BALB/c or B6 mice. This dengue-2-virus-infected mouse system accompanied by thrombocytopenia and anti-platelet antibody will be a valuable model to study the pathogenicity of dengue virus infection.

scholarly journals Frequency of Dengue virus Infection among Febrile Patients of Lahore

2017 ◽  
Vol 9 (10) ◽  
pp. 212
Author(s):  
M. Salman Munir Malik ◽  
Fahad Javed ◽  
Muhammad Wasim ◽  
Maham Ulfat ◽  
Sana Arshad ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Specific Antigen ◽  
Clinical Manifestations ◽  
P Value ◽  
Age Group ◽  
Dengue Virus Infection ◽  
Ns1 Antigen ◽  
Lahore City

BACKGROUND: Dengue virus infection is one of the major global public health problems. The infection usually occurs with clinical manifestations ranging from an asymptomatic or mild febrile illness as classical dengue fever to the potentially life-threatening illness, dengue hemorrhagic fever and dengue shock syndrome. The objective of the current study was to observe the frequency and diagnosis of primary or secondary dengue viral infection among individuals in Lahore city, Pakistan.METHODS: Study subjects were identified for the presence of dengue diagnostic markers including NS1 antigen, IgM and IgG antibody. The dengue specific antigen NS1 was detected by immonochromatography, while dengue specific antibodies (IgM and IgG) were measured through ELISA.RESULTS: Total 98 (56%) out of 175 febrile cases were found infected by dengue virus. From total 98 confirmed dengue cases, NS1 antigen was detected in 59 (60.20%), IgM antibodies were present in 74 (75.51%) and IgG antibodies were detected in 40 (40.81%) individuals. Statistical analysis reveals correlation of NS1 antigen and IgM antibody among dengue patients with significant P-value (P < 0.01). Results indicated that 58 (59%) were infected by primary infection and 40 (41%) were infected by secondary infection. The most effected age group was 21-30 years (51.02%) and least effected age group was <10 years (3.06%). Males were observed higher in number 61 (62%) as compare to females 37 (38%). Overall, the frequency of dengue virus infections were 56% among undifferentiated febrile patients in Lahore city, Pakistan.CONCLUSION: Dengue is found endemic in city population with increased incidence in monsoon and post monsoon. Constant vigilance of patients and dengue vector control awareness programs among public and health care officials could support in combating dengue.

scholarly journals Protective Capacity of the Human Anamnestic Antibody Response during Acute Dengue Virus Infection

2016 ◽  
Vol 90 (24) ◽  
pp. 11122-11131 ◽  
Author(s):  
Meihui Xu ◽  
Roland Züst ◽  
Ying Xiu Toh ◽  
Jennifer M. Pfaff ◽  
Kristen M. Kahle ◽  
...  
Keyword(s):  
Virus Infection ◽  
Antibody Response ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Immune Memory ◽  
Protective Capacity ◽  
Dengue Virus Infection ◽  
Immune Correlates

ABSTRACT Half of the world's population is exposed to the risk of dengue virus infection. Although a vaccine for dengue virus is now available in a few countries, its reported overall efficacy of about 60% is not ideal. Protective immune correlates following natural dengue virus infection remain undefined, which makes it difficult to predict the efficacy of new vaccines. In this study, we address the protective capacity of dengue virus-specific antibodies that are produced by plasmablasts a few days after natural secondary infection. Among a panel of 18 dengue virus-reactive human monoclonal antibodies, four groups of antibodies were identified based on their binding properties. While antibodies targeting the fusion loop of the glycoprotein of dengue virus dominated the antibody response, two smaller groups of antibodies bound to previously undescribed epitopes in domain II of the E protein. The latter, largely serotype-cross-reactive antibodies, demonstrated increased stability of binding at pH 5. These antibodies possessed weak to moderate neutralization capacity in vitro but were the most efficacious in promoting the survival of infected mice. Our data suggest that the cross-reactive anamnestic antibody response has a protective capacity despite moderate neutralization in vitro and a moderate decrease of viremia in vivo . IMPORTANCE Antibodies can protect from symptomatic dengue virus infection. However, it is not easy to assess which classes of antibodies provide protection because in vitro assays are not always predictive of in vivo protection. During a repeat infection, dengue virus-specific immune memory cells are reactivated and large amounts of antibodies are produced. By studying antibodies cloned from patients with heterologous secondary infection, we tested the protective value of the serotype-cross-reactive “recall” or “anamnestic” response. We found that results from in vitro neutralization assays did not always correlate with the ability of the antibodies to reduce viremia in a mouse model. In addition, a decrease of viremia in mice did not necessarily improve survival. The most protective antibodies were stable at pH 5, suggesting that antibody binding in the endosomes, after the antibody-virus complex is internalized, might be important to block virus spread in the organism.

scholarly journals GAMBARAN NILAI HEMATOKRIT DAN LAJU ENDAP DARAH PADA ANAK DENGAN INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Sitti S. P. Kamuh ◽  
Arthur E. Mongan ◽  
Maya F. Memah
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Clinical Symptoms ◽  
Clinical Manifestations ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Dengue Virus Infection ◽  
Erythrocyte Sedimentation

Abstract: Dengue is a major public health problem that can be found throughout the tropical and subtropical countries around the world. Spectrum of clinical manifestations of dengue virus infection varies greatly. In addition to clinical symptoms, the diagnosis of dengue virus infection need to be supported by blood tests such as hematocrit and erythrocyte sedimentation rate. This study aimed to determine the hematocrit value and erythrocyte sedimentation rate (ESR) in children with dengue virus infection in Manado. This study used a cross-sectional design and was conducted from Desember 2014 to January 2015 at GMIM Pancaran Kasih, Advent, and Robert Wolter Mongisidi hospitals in Manado. There were 37 patients that fulfilled the inclusion criteria, consisted of 17 males and 20 females. The results showed that of 37 patients, there were 36 with hematocrit within normal limits. Moreover, of 37 patients only 6 patients performed the ESR examination; only 1 child had a rapid ESR result (> 15mm/h). Conclusion: In this study, most of the pediatric patients with dengue virus infection in Manado had normal hematocrit. Of 6 patients who had performed ESR test, only 1 had rapid ESR.Keywords: dengue, hematocrit, erythrocyte sedimentation rateAbstrak: Dengue merupakan masalah kesehatan masyarakat yang utama dan dapat ditemui diseluruh daerah tropis dan subtropis di seluruh dunia. Spektrum manifestasi klinis infeksi virus dengue sangat bervariasi. Selain gejala klinis, diagnosis infeksi virus dengue perlu ditunjang hasil uji darah di laboratorium antara lain hematokrit dan laju endap darah (LED). Penelitian ini bertujuan untuk mengetahui nilai hematokrit dan LED pada anak dengan infeksi virus dengue di Manado. Penelitian ini menggunakan desain potong lintang, dilakukan pada bulan Desember 2014 sampai Januari 2015 di RSU GMIM Pancaran Kasih Manado, RS Advent Manado, dan RSAD Robert Wolter Mongisidi Teling17 anak laki-laki dan 20 anak perempuan. Hasil penelitian memperlihatkan 36 dari 37 pasien mempunyai hematokrit normal. Dasri 37 pasien, hanya 6 anak yang menjalani pemeriksaan LED; hanya 1 anak dengan LED cepat (> 15mm/jam). Simpulan: Pada studi ini, sebagian besar pasien anak dengan infeksi virus dengue di Manado mempunyai nilai hematokrit normal. Dari 6 pasien yang dilakukan pemeriksaan LED, 1 anak mempunyai hasil LED cepat.Kata kunci: dengue, hematrokrit, laju endap darah.

scholarly journals Diagnostic value of newborn foot length to predict gestational age

2017 ◽  
Vol 57 (4) ◽  
pp. 181
Author(s):  
Mutia Farah Fawziah ◽  
Bambang Soebagyo ◽  
Dwi Hidayah
Keyword(s):  
Likelihood Ratio ◽  
Gestational Age ◽  
Predictive Value ◽  
Positive Likelihood Ratio ◽  
Negative Likelihood Ratio ◽  
Diagnostic Value ◽  
Full Term ◽  
Anthropometric Measurement ◽  
Diagnostic Study ◽  
Foot Length

Background  Identification of gestational age, especially within 48 hours of birth, is crucial for newborns, as the earlier preterm status is detected, the earlier the child can receive optimal management. Newborn foot length is an anthropometric measurement which is easy to perform, inexpensive, and potentially efficient for predicting gestational age.Objective  To analyze the diagnostic value of newborn foot length in predicting gestational age.Methods  This diagnostic study was performed between October 2016 and February 2017 in the High Care Unit of Neonates at Dr. Moewardi General Hospital, Surakarta. A total of 152 newborns were consecutively selected and underwent right foot length measurements before 96 hours of age. The correlation between newborn foot length to classify as full term and gestational age was analyzed with Spearman’s correlation test because of non-normal data distribution. The cut-off point of newborn foot length was calculated by receiver operating characteristic (ROC) curve and diagnostic values of newborn foot length were analyzed by 2 x 2 table with SPSS 21.0 software.Results There were no significant differences between male and female newborns in terms of gestational age, birth weight, choronological age, and newborn foot length (P>0.05). Newborn foot length and gestational age had a significant correlation (r=0.53; P=0.000). The optimal cut-off newborn foot length to predict full term status was 7.1 cm. Newborn foot length below 7.1 cm had sensitivity 75%, specificity 98%, positive predictive value 94.3%, negative predictive value 90.6%, positive likelihood ratio 40.5, negative likelihood ratio 0.25, and post-test probability 94.29%, to predict preterm status in newborns.Conclusion  Newborn foot length can be used to predict gestational age, especially for the purpose of differentiating between preterm and full term newborns.

scholarly journals Clinical Evaluation of a Rapid Immunochromatographic Test for the Diagnosis of Dengue Virus Infection

1998 ◽  
Vol 5 (3) ◽  
pp. 407-409 ◽  
Author(s):  
Chew Theng Sang ◽  
Lim Siew Hoon ◽  
Andrea Cuzzubbo ◽  
Peter Devine
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Rapid Test ◽  
Immunoglobulin M ◽  
Immunochromatographic Test ◽  
Dengue Virus Infection ◽  
Hemagglutination Inhibition Assay ◽  
Specific Immunoglobulin ◽  
Paired Serum

ABSTRACT A rapid immunochromatographic test was compared to the hemagglutination inhibition assay for separate determinations of dengue virus-specific immunoglobulin M (IgM) and IgG levels in paired serum specimens from 92 patients (34 with primary dengue virus infection, 35 with secondary dengue virus infection, and 23 without dengue virus infection). The rapid test showed 99% sensitivity in the diagnosis of dengue virus infection. The majority (30 of 34 [88%]) of patients with primary infection showed positive IgM but negative IgG, while 34 of 35 (97%) patients with secondary infection showed positive IgG with or without IgM. Specificity in nonflavivirus infections was 96% (1 of 23 positive). The rapid test should be a useful aid in rapid diagnosis of dengue virus infection.

scholarly journals Correlation of Serotype-Specific Dengue Virus Infection with Clinical Manifestations

2012 ◽  
Vol 6 (5) ◽  
pp. e1638 ◽  
Author(s):  
Eric S. Halsey ◽  
Morgan A. Marks ◽  
Eduardo Gotuzzo ◽  
Victor Fiestas ◽  
Luis Suarez ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestations ◽  
Dengue Virus Infection

scholarly journals FEVER AS INDICATOR TO SECONDARY INFECTION IN DENGUE VIRAL INFECTION

2018 ◽  
Vol 7 (1) ◽  
pp. 21
Author(s):  
Soegeng Soegijanto ◽  
Sufiandika Nuryandari ◽  
Siti Churrotin ◽  
Teguh Hari Sucipto
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Viral Infection ◽  
Dengue Fever ◽  
Clinical Manifestation ◽  
Secondary Infection ◽  
Salmonella Typhi ◽  
Severe Dengue ◽  
Concurrent Infection ◽  
Dengue Virus Infection

Dengue Virus Infections are distributed in tropical and sub-tropical regions and transmitted by the mosquitoes such as Aedes aegypti and Aedes albopictus. Dengue virus can cause dengue fever, dengue hemorrhagic fever and dengue shock syndrome or dengue and severe dengue classified by World Health Organization. Beside it concurrent infection virus salmonella had been found some cases who showed fever more than 7 days. Concurrent infection with two agents can result in an illness having overlapping symptoms creating a diagnostic dilemma for treating physician, such as dengue fever with typhoid fever. The aim of this research is detection of dengue virus and secondary infection with Salmonella typhi in patients suspected dengue virus infection. Detection of dengue virus and Salmonella typhi using immunochromatography test such as NS1, IgG/IgM for dengue virus infection, and IgM/IgG Salmonella and blood culture. The fifty children with dengue virus infection came to Soerya hospital and 17 cases suspected dengue virus infection, five cases showed a positive NS1 on the second day of fever and one case concurrent with clinical manifestation of convulsi on the third days of fever there were five cases only showed positive. It was showed in this study that on the fourth to six day of fever in dengue virus infection accompanied by antibody IgM & IgG dengue. There were 12 cases showed the clinical manifestation of concurrent dengue viral infection and Salmonella, all of them showed a mild clinical manifestation and did not show plasma leakage and shock. In this study we found the length of stay of concurrent Dengue Virus Infection and Salmonella infection is more than 10 days. These patients were also more likely to have co-existing haemodynamic disturbances and bacterial septicaemia which would have required treatment with inotropes and antibiotics. This idea is very important to make update dengue viral management to decrease mortality in outbreak try to gain new prevention method before the occurrence of outbreak.

Diagnostic Value of International Ovarian Tumor Analysis Simple Rules (IOTA-SR) In Iranian Patients with Ovarian Mass

2021 ◽  
Vol 15 (5) ◽  
pp. 1647-1651
Author(s):  
Mohammad Reza Babaei ◽  
Mohammadreza Khaleghi ◽  
Manizhe Ataee Kachuee ◽  
Farnaz Ardiyani ◽  
Setare Nassiri
Keyword(s):  
Ovarian Cancer ◽  
Likelihood Ratio ◽  
Predictive Value ◽  
Positive Likelihood Ratio ◽  
Negative Likelihood Ratio ◽  
Diagnostic Value ◽  
Ovarian Tumors ◽  
Benign Tumors ◽  
Ovarian Mass ◽  
Iranian Patients

Background: Among gynecologic malignancies, ovarian cancer has the highest mortality rate per case. The most important prognostic feature of ovarian cancer is an early diagnosis. The IOTA-SR is an ultrasound exam criterion for classifying benign or malignant ovarian tumors, which could be used effectively by an inexperienced radiologist. This study aimed to evaluate the diagnostic value of IOTA-SR in Iranian patients with ovarian tumors. Methods: In this cross-sectional study, considering inclusion and exclusion criteria, 60 females enrolled (from 2019-1 to 2019-12). Patients were evaluated with ultrasound by an experienced radiologist. Tumors were classified, as benign or malignant according to the IOTA-SR criteria. After laparotomy, histological and ultrasond examination findings were compared. Results: According to histopathologic results, the prevalence of malignancy was 63.3% (38 malignant and 22 benign tumors). The IOTA-SR could be applied in all the participants as 21 tumors (35%) were classified as benign and 39 (65%) as malignant. Compared with histopathologic results, the IOTA-SR yielded 36 true positives, three false positives, 19 true negatives, and two false negatives. Thus, assessing ovarian mass using IOTA-SR achieved a sensitivity of 94.7%, a specificity of 86.3%, a positive predictive value of 92.3%, a negative predictive value of 90.47%, positive likelihood ratio of 6.91, negative likelihood ratio of 0.091, overall accuracy of 91.6%, and kappa coefficient of 0.819 (P<0.001). Conclusions: IOTA-SR criteria have a high diagnostic value in differentiation of the malignant and benign ovarian tumors and can be applied in daily practice.

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