scholarly journals FEVER AS INDICATOR TO SECONDARY INFECTION IN DENGUE VIRAL INFECTION

2018 ◽  
Vol 7 (1) ◽  
pp. 21
Author(s):  
Soegeng Soegijanto ◽  
Sufiandika Nuryandari ◽  
Siti Churrotin ◽  
Teguh Hari Sucipto
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Viral Infection ◽  
Dengue Fever ◽  
Clinical Manifestation ◽  
Secondary Infection ◽  
Salmonella Typhi ◽  
Severe Dengue ◽  
Concurrent Infection ◽  
Dengue Virus Infection

Dengue Virus Infections are distributed in tropical and sub-tropical regions and transmitted by the mosquitoes such as Aedes aegypti and Aedes albopictus. Dengue virus can cause dengue fever, dengue hemorrhagic fever and dengue shock syndrome or dengue and severe dengue classified by World Health Organization. Beside it concurrent infection virus salmonella had been found some cases who showed fever more than 7 days. Concurrent infection with two agents can result in an illness having overlapping symptoms creating a diagnostic dilemma for treating physician, such as dengue fever with typhoid fever. The aim of this research is detection of dengue virus and secondary infection with Salmonella typhi in patients suspected dengue virus infection. Detection of dengue virus and Salmonella typhi using immunochromatography test such as NS1, IgG/IgM for dengue virus infection, and IgM/IgG Salmonella and blood culture. The fifty children with dengue virus infection came to Soerya hospital and 17 cases suspected dengue virus infection, five cases showed a positive NS1 on the second day of fever and one case concurrent with clinical manifestation of convulsi on the third days of fever there were five cases only showed positive. It was showed in this study that on the fourth to six day of fever in dengue virus infection accompanied by antibody IgM & IgG dengue. There were 12 cases showed the clinical manifestation of concurrent dengue viral infection and Salmonella, all of them showed a mild clinical manifestation and did not show plasma leakage and shock. In this study we found the length of stay of concurrent Dengue Virus Infection and Salmonella infection is more than 10 days. These patients were also more likely to have co-existing haemodynamic disturbances and bacterial septicaemia which would have required treatment with inotropes and antibiotics. This idea is very important to make update dengue viral management to decrease mortality in outbreak try to gain new prevention method before the occurrence of outbreak.

scholarly journals Serotype and Clinical Performance of Dengue Virus Infection on the Year 2009

2010 ◽  
Vol 1 (2) ◽  
pp. 55 ◽  
Author(s):  
Soegeng Soegijanto ◽  
Widodo Darmowandowo ◽  
Amor Peraten Ginting ◽  
Atsushi Yamanaka
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestation ◽  
Clinical Performance ◽  
Secondary Infection ◽  
Severe Disease ◽  
Dengue Shock Syndrome ◽  
Severe Dengue ◽  
Rt Pcr ◽  
Dengue Virus Infection

Dengue hemorrhagic fever is one of the important health problem in Indonesia, mortality rate is becoming decrease but many dengue shock syndrome cases is very difficult to be help. Previous study showed that some of DEN 2 and DEN 3 virus cases could show a clinical performance of severe dengue virus infection such as dengue shock syndrome. There are four serotype of dengue virus infection can cause primary and secondary infection. The aim of this research is to know the relationship between clinical performance of dengue virus infection and serotype dengue virus and also to know the role of primary and secondary infection and age of dengue virus cases. A prospective analytic observational study, which was conducted in Dr. Soetomo hospital since January 2009. RT-PCR was used to attempt to identify the infecting serotype from dengue virus isolated using vero cell. Antibody responses were measured by ELISA and clinical manifestation were measured with the WHO criteria 1997. Dengue serotype identification by RT-PCR was 70 patients. Virus types were DEN-2 65(92.8%), DEN-1 3(4.2%), and DEN-3 2(2.8%). Patients with DEN-1 genotype IV were more trend severe disease DSS and unusual infection. Commanly usually secondary exposure cause more severe clinical manifestation than primary exposure (p = 0.035) but in this study found that all of DEN-1 genotype IV, primary or secondary infection to show severe clinical manifestation of dengue virus infection. We can conclude that DEN-2 was the most dominant serotype in Dr. Soetomo Hospital. On Primary and secondary infection, DEN-1 genotype IV showing more severe than DEN-2 and DEN-3.

scholarly journals Retrospective Seroepidemiology study of dengue virus infection in Taiwan

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ying-Hsuan Lee ◽  
Yu-Chia Hsieh ◽  
Chih-Jung Chen ◽  
Tzou-Yien Lin ◽  
Yhu-Chering Huang
Keyword(s):  
General Population ◽  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Health Concern ◽  
Age Group ◽  
Dengue Virus Infection ◽  
Recent Infection ◽  
Seropositive Rate ◽  
Significant Difference

Abstract Background Dengue virus infection has been an important and serious public health concern in Taiwan, where local outbreaks of dengue fever occurred almost every year. To our knowledge, no nationwide investigation has been carried out to determine the actual extent of infection in the general population. Methods A total of 1308 random serum samples were collected from the general population in Taiwan in 2010. The antibody-captured enzyme-linked immunosorbent assays were used to detect DENV-specific IgM and IgG. Demographics data were used for risk analysis. Results The weighted overall seroprevalence was 1.96% for anti-DENV IgM, and 3.4% for anti-DENV IgG, respectively. A significant rise of DENV IgG seropositive rate had been noted since late adulthood stage, from 1.1% at the age group of 50–59 years to 7.6% at the age group of 60–69 years. For people aged over 70 years, the seropositive rate reached 19%. Age, nationality, and regions of residency were associated with the IgG seropositivity. There was no statistically significant difference in seroprevalence of anti-Dengue IgM, indicating recent infection, among univariate predictors we proposed, including gender, age, residency, nationality, and household size. Conclusions Our results indicated that the majority of population in Taiwan born after 1940 is naive to dengue virus and the prevalence of IgG antibody against dengue virus rises with age. Nationality, and regions of residency are associated with the exposure of population to infection by dengue viruses. Further studies are needed to realize the current situation of seroprevalence of dengue fever in Taiwan.

Case Report: Transmission of Dengue Virus from a Deceased Donor to a Kidney Transplant Recipient Previously Infected by Dengue Virus

2021 ◽  
Author(s):  
Arnaud Lecadieu ◽  
Laura Teysseyre ◽  
Kevin Larsen ◽  
Charles Vidal ◽  
Margot Caron ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Kidney Transplant ◽  
Kidney Transplant Recipient ◽  
Deceased Donor ◽  
Severe Dengue ◽  
Reunion Island ◽  
Dengue Virus Infection ◽  
First Case ◽  
Réunion Island

Since 2018, a dengue epidemic has been ongoing in the French overseas department of Reunion Island, in the Indian Ocean, with more than 25,000 serologically confirmed cases. Currently, three dengue serotypes have been identified in Réunion Island (DENV-1, DENV-2, and DENV-3) progressing in the form of epidemic outbreaks. This arbovirus is mainly transmitted by mosquitoes of the genus Aedes and may be responsible for serious clinical forms. To date, very few cases of kidney transplant–related dengue virus infection have been described. Here we report the first case of severe dengue virus infection related to kidney transplantation from a patient previously infected with dengue. Testing for dengue fever with PCR search in donor’s urine may help complete the pretransplant assessment in areas where this disease occurs.

scholarly journals AWARENESS OF USING RINGER LACTAT SOLUTION IN DENGUE VIRUS INFECTION CASES COULD INDUCE SEVERITY

2013 ◽  
Vol 4 (4) ◽  
pp. 35
Author(s):  
Soegeng Soegijanto ◽  
Desiana W. Sari ◽  
Atsushi Yamanaka ◽  
Tomohiro Kotaki ◽  
Masanori Kameoka ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestation ◽  
Liver Damage ◽  
Acetate Solution ◽  
Ringer Lactate ◽  
Primary Case ◽  
Dengue Virus Infection ◽  
Group B ◽  
Lactate Solution

Background: In 2012, serotype of Dengue Virus had changed from Den-2 and Den-3 to Den-1. In 5–10 years ago, serotype of Den-1 case showed a mild clinical manifestation; but now as a primary case it can also show severe clinical manifestation. One of indicator is an increasing liver enzyme, AST and ALT, with level more than 100–200 U/L. Aim: To getting a better solutions for this problem. Method: Obsevasional Study had been done in medical faculty of Airlangga University (Dr. Soetomo and Soerya hospital) Surabaya on Mei–August 2012. There were 10 cases of dengue virus infection were studied, 5 cases got Ringer Acetate solution (Group A) and 5 cases got Ringer Lactate solution (Group B). The diagnosis was based on criteria WHO 2009. Result: Five cases of Dengue Virus Infection had showed a liver damage soon after using Ringer Lactate solution; AST and ALT were increasing more than 100–200 U/L; but the other 5 cases showed better condition. It might be due to use Ringer Acetate that did not have effect for inducing liver damage. By managing carefully, all of the cases had shown full recovery and healthy condition when being discharged. Conclusion: Using Ringer Acetate as fluid therapy in Dengue Virus Infection is better to prevent liver damage than using Ringer Lactate.

scholarly journals Seroepidemiology of dengue virus infection among adults during the ending phase of a severe dengue epidemic in southern Taiwan, 2015

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yu-Wen Chien ◽  
Hsiang-Min Huang ◽  
Tzu-Chuan Ho ◽  
Fan-Chen Tseng ◽  
Nai-Ying Ko ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Severe Dengue ◽  
Dengue Virus Infection ◽  
Southern Taiwan ◽  
Dengue Epidemic

scholarly journals Current Understanding of the Pathogenesis of Dengue Virus Infection

2020 ◽  
Author(s):  
Puneet Bhatt ◽  
Sasidharan Pillai Sabeena ◽  
Muralidhar Varma ◽  
Govindakarnavar Arunkumar
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Virus Infection ◽  
Dengue Virus ◽  
Genetic Factors ◽  
Host Immune Response ◽  
Severe Dengue ◽  
Ns1 Protein ◽  
Dengue Virus Infection ◽  
Antibody Dependent Enhancement

AbstractThe pathogenesis of dengue virus infection is attributed to complex interplay between virus, host genes and host immune response. Host factors such as antibody-dependent enhancement (ADE), memory cross-reactive T cells, anti-DENV NS1 antibodies, autoimmunity as well as genetic factors are major determinants of disease susceptibility. NS1 protein and anti-DENV NS1 antibodies were believed to be responsible for pathogenesis of severe dengue. The cytokine response of cross-reactive CD4+ T cells might be altered by the sequential infection with different DENV serotypes, leading to further elevation of pro-inflammatory cytokines contributing a detrimental immune response. Fcγ receptor-mediated antibody-dependent enhancement (ADE) results in release of cytokines from immune cells leading to vascular endothelial cell dysfunction and increased vascular permeability. Genomic variation of dengue virus and subgenomic flavivirus RNA (sfRNA) suppressing host immune response are viral determinants of disease severity. Dengue infection can lead to the generation of autoantibodies against DENV NS1antigen, DENV prM, and E proteins, which can cross-react with several self-antigens such as plasminogen, integrin, and platelet cells. Apart from viral factors, several host genetic factors and gene polymorphisms also have a role to play in pathogenesis of DENV infection. This review article highlights the various factors responsible for the pathogenesis of dengue and also highlights the recent advances in the field related to biomarkers which can be used in future for predicting severe disease outcome.

scholarly journals Protective Capacity of the Human Anamnestic Antibody Response during Acute Dengue Virus Infection

2016 ◽  
Vol 90 (24) ◽  
pp. 11122-11131 ◽  
Author(s):  
Meihui Xu ◽  
Roland Züst ◽  
Ying Xiu Toh ◽  
Jennifer M. Pfaff ◽  
Kristen M. Kahle ◽  
...  
Keyword(s):  
Virus Infection ◽  
Antibody Response ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Immune Memory ◽  
Protective Capacity ◽  
Dengue Virus Infection ◽  
Immune Correlates

ABSTRACT Half of the world's population is exposed to the risk of dengue virus infection. Although a vaccine for dengue virus is now available in a few countries, its reported overall efficacy of about 60% is not ideal. Protective immune correlates following natural dengue virus infection remain undefined, which makes it difficult to predict the efficacy of new vaccines. In this study, we address the protective capacity of dengue virus-specific antibodies that are produced by plasmablasts a few days after natural secondary infection. Among a panel of 18 dengue virus-reactive human monoclonal antibodies, four groups of antibodies were identified based on their binding properties. While antibodies targeting the fusion loop of the glycoprotein of dengue virus dominated the antibody response, two smaller groups of antibodies bound to previously undescribed epitopes in domain II of the E protein. The latter, largely serotype-cross-reactive antibodies, demonstrated increased stability of binding at pH 5. These antibodies possessed weak to moderate neutralization capacity in vitro but were the most efficacious in promoting the survival of infected mice. Our data suggest that the cross-reactive anamnestic antibody response has a protective capacity despite moderate neutralization in vitro and a moderate decrease of viremia in vivo . IMPORTANCE Antibodies can protect from symptomatic dengue virus infection. However, it is not easy to assess which classes of antibodies provide protection because in vitro assays are not always predictive of in vivo protection. During a repeat infection, dengue virus-specific immune memory cells are reactivated and large amounts of antibodies are produced. By studying antibodies cloned from patients with heterologous secondary infection, we tested the protective value of the serotype-cross-reactive “recall” or “anamnestic” response. We found that results from in vitro neutralization assays did not always correlate with the ability of the antibodies to reduce viremia in a mouse model. In addition, a decrease of viremia in mice did not necessarily improve survival. The most protective antibodies were stable at pH 5, suggesting that antibody binding in the endosomes, after the antibody-virus complex is internalized, might be important to block virus spread in the organism.

scholarly journals Sero-diagnosis of Dengue virus in Different Hospitals of Nepal

2013 ◽  
Vol 1 (2) ◽  
pp. 58-62 ◽  
Author(s):  
Y Shah ◽  
G Khadka ◽  
GP Gupta ◽  
N Adhikari ◽  
A Poudel ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Control Measure ◽  
Public Health Problem ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dengue Virus Infection ◽  
Capture Elisa ◽  
Occupation Group ◽  
Hilly Region

INTRODUCTION: Dengue fever (DF) is an emerging mosquito borne viral disease and important public health problem in low land Terai region which is also moving towards hilly region Nepal. This study was designed to determine the sero-prevalence of dengue virus infection in patients visiting hospitals of Nepal. MATERIALS AND METHODS: This study was conducted during period (June-November) of 2010 in Nepalese patients with fever visiting hospitals of Birganj, Damouli, Biratanagar, Dhading Besi and Chitwan. The sero-prevalence of dengue virus specific IgM was determined by enzyme linked immunosorbent assay (ELISA). Serum samples were collected from 289 patients visiting hospitals with history of fever and clinically suspected dengue fever. RESULTS: The anti-dengue IgM positivity was found to be 8.99%. The positive dengue cases were higher in male (10.8%) as compared to female (7.1%) though it was not statistically significant (P>0.05). Among different age groups, the highest positive cases (12.3%) were from age group below 15 years followed by above 50 years 8.3%. Out of 5 hospitals, the highest positive cases were in Tanahu hospital, Damouli (23.8%) followed by Bharatpur hospital and Chitwan (22.2%). Age and gender were found to be independent predictors. The highest numbers of dengue positive cases were in occupation group business (13.3%) followed by agriculture (12.7%). CONCLUSIONS: Prevalence of dengue virus infection is increasing and proper control measure should be provided. IgM capture ELISA was used for laboratory analysis and remains as a reliable and inexpensive method for the diagnosis of dengue. Hence, the IgM capture ELISA has become the most accepted technique for the diagnosis of dengue in developing countries like Nepal. DOI: http://dx.doi.org/10.3126/ijim.v1i2.7003 Int J Infect Microbiol 2012;1(1):58-62

scholarly journals Clinical Evaluation of a Rapid Immunochromatographic Test for the Diagnosis of Dengue Virus Infection

1998 ◽  
Vol 5 (3) ◽  
pp. 407-409 ◽  
Author(s):  
Chew Theng Sang ◽  
Lim Siew Hoon ◽  
Andrea Cuzzubbo ◽  
Peter Devine
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Rapid Test ◽  
Immunoglobulin M ◽  
Immunochromatographic Test ◽  
Dengue Virus Infection ◽  
Hemagglutination Inhibition Assay ◽  
Specific Immunoglobulin ◽  
Paired Serum

ABSTRACT A rapid immunochromatographic test was compared to the hemagglutination inhibition assay for separate determinations of dengue virus-specific immunoglobulin M (IgM) and IgG levels in paired serum specimens from 92 patients (34 with primary dengue virus infection, 35 with secondary dengue virus infection, and 23 without dengue virus infection). The rapid test showed 99% sensitivity in the diagnosis of dengue virus infection. The majority (30 of 34 [88%]) of patients with primary infection showed positive IgM but negative IgG, while 34 of 35 (97%) patients with secondary infection showed positive IgG with or without IgM. Specificity in nonflavivirus infections was 96% (1 of 23 positive). The rapid test should be a useful aid in rapid diagnosis of dengue virus infection.

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