scholarly journals GLUCOSE LEVEL ANALYSIS ON STORED PACKED RED CELLS

2018 ◽  
Vol 24 (2) ◽  
pp. 117
Author(s):  
Melissa Heidy Wongsari ◽  
Rachmawati Muhiddin ◽  
Mansyur Arif
Keyword(s):  
Lactic Acid ◽  
Glucose Level ◽  
Cell Metabolism ◽  
Blood Bank ◽  
Red Cells ◽  
Red Cell ◽  
Glucose Levels ◽  
Red Cell Metabolism ◽  
Acid Accumulation ◽  
Level Analysis

The main source of energy for red cell metabolism is glucose via glycolytic pathway. Red cells metabolism slows down during storage at 2–6OC. Biochemical changes during storage are called storage lesions, i.e. decreased pH, glucose, and ATP, lactic acid accumulation, and loss of red cells function. Samples taken from the tip of PRC bags in CPDA-1 of the same code are divided into four sections, and stored in the blood bank refrigerator at 2–6OC. Glucose level is measured using ABX Pentra 400 (Horiba, Japan) on storage day 3 as a control, day 7, day 14, and day 21. Glucose levels during storage decreased significantly between day 3, and day 7 (p < 0.001), between day 7, and day 14 (p < 0.001), and between day 14, and 21 (p < 0.001). Glucose levels of Packed Red Cells (PRC) decrease during storage. Glycolysis occurs during storage although metabolism slows down.

RED CELL METABOLISM IN THE PREMATURE INFANT

PEDIATRICS ◽  
1965 ◽  
Vol 36 (1) ◽  
pp. 104-112
Author(s):  
Frank A. Oski ◽  
J. Lawrence Naiman
Keyword(s):  
Premature Infant ◽  
Premature Infants ◽  
Metabolic Profile ◽  
Cell Metabolism ◽  
Glucose Consumption ◽  
Red Cells ◽  
Red Cell ◽  
Term Infants ◽  
Atp Levels ◽  
Red Cell Metabolism

The erythrocytes of premature infants, term infants, and adults were studied with respect to ATP levels, ATP stability, glucose consumption, glutathione stability, and the tendency to develop morphologic abnormalities during short periods of incubation. The erythrocytes of both the premature and term infants had higher ATP levels and glucose consumption, greater ATP and glutathione instability, and more marked morphologic abnormalities than the adult red cells. The erythrocytes of the premature infants showed the greatest degrees of abnormality. It is suggested that the premature infant at birth possesses a metabolic profile in his erythrocytes which results in ATP instability.

RED CELL METABOLISM IN THE NEWBORN INFANT. IV. TRANSMEMBRANE POTASSIUM FLUX

PEDIATRICS ◽  
1969 ◽  
Vol 43 (3) ◽  
pp. 396-401
Author(s):  
Stuart F. Blum ◽  
Frank A. Oski
Keyword(s):  
Membrane Permeability ◽  
Cell Metabolism ◽  
Newborn Infants ◽  
Red Cell ◽  
Membrane Injury ◽  
Red Cell Metabolism ◽  
Potassium Flux ◽  
Potassium Influx ◽  
Normal Adults ◽  
Erythrocytes Of Newborn Infants

Measurements of transmembrane potassium flux in the erythrocytes of newborn infants and normal adults demonstrated an increased net loss of potassium in the infants. This loss appeared to be a consequence of decreased active potassium influx rather than an increased membrane permeability. These studies suggest that the erythrocytes of newborn infants may be more vulnerable to destruction resulting from membrane injury because of their reduced capacity for active transport.

scholarly journals Effect of oxalate and malonate on red cell metabolism

Blood ◽  
1987 ◽  
Vol 70 (5) ◽  
pp. 1389-1393
Author(s):  
E Beutler ◽  
L Forman ◽  
C West
Keyword(s):  
Pyruvate Kinase ◽  
Cell Metabolism ◽  
Inhibitory Effect ◽  
Red Cells ◽  
Red Cell ◽  
Acid Analogue ◽  
2,3 Dpg

The addition of oxalate to blood stored in Citrate-phosphate-dextrose (CPD) produces a marked improvement in 2,3-diphosphoglycerate (2,3-DPG) preservation; an increase in 2,3-DPG levels can also be documented in short-term incubation studies. Oxalate is a potent in vitro inhibitor of red cell lactate dehydrogenase, monophosphoglycerate mutase, and pyruvate kinase (PK). In the presence of fructose 1,6-diphosphate the latter inhibitory effect is competitive with phospho(enol)pyruvate (PEP). Determination of the levels of intermediate compounds in red cells incubated with oxalate suggest the presence of inhibition at the PK step, indicating that this is the site of oxalate action. Apparent inhibition at the glyceraldehyde phosphate dehydrogenase step is apparently due to an increase in the NADH/NAD ratio. Oxalate had no effect on the in vivo viability of rabbit red cells stored in CPD preservatives for 21 days. Greater understanding of the toxicity of oxalate is required before it can be considered suitable as a component of preservative media, but appreciation of the mechanism by which it affects 2,3-DPG levels may be important in design of other blood additives. Malonate, the 3-carbon dicarboxylic acid analogue of oxalate late did not inhibit pyruvate kinase nor affect 2,3-DPG levels.

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