scholarly journals CLONAL PROPAGATION OF Gypsophila aretioides, AN IDEAL ROCK GARDEN PLANT SPECIES

2019 ◽  
Vol 18 (1) ◽  
pp. 39-45
Author(s):  
Leila Samiei ◽  
Mahboubeh Davoudi Pahnekolayi ◽  
Zahra Karimian
Keyword(s):  
Survival Rate ◽  
Clonal Propagation ◽  
Ground Cover ◽  
Root Induction ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Garden Plant ◽  
Ornamental Species

Gypsophila aretioides, a cushion form evergreen plant, is a high potential wild species ideal for the use in rock garden, or as a ground cover in sunny dry areas. This plant has the competence to be developed as a new ornamental species. The purpose of this experiment was to provide an efficient micropropagation protocol for G. aretioides in order to facilitate the availability of this species for further studies of domestication. The influence of various concentrations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated for multiplication stage. TDZ at low concentration of 0.05 mg dm−3 resulted in the maximum shoot (9.7) and leaf (42.3) number. The shoots were best rooted on MS medium containing 0.6 mg dm–3 indolebutyric acid (IBA) with 7.8 roots per shoot. Despite achievement of a successful protocol for in vitro multiplication and root induction of Gypsophila, low survival rate was obtained when rooted explants were exposed to ex vitro conditions. This is an important issue, which requires particular consideration and further studies. The possible reasons contributing to the low acclimatization rate of this species are being discussed.

scholarly journals An Improved Micropropagation Protocol byEx VitroRooting ofPassiflora edulisSims. f.flavicarpaDeg. through Nodal Segment Culture

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari ◽  
C. P. Ravindran
Keyword(s):  
Survival Rate ◽  
Clonal Propagation ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Nodal Segment ◽  
Ms Medium ◽  
Ex Vitro ◽  
Passiflora Edulis ◽  
Half Strength

A procedure for rapid clonal propagation ofPassiflora edulisSims. f.flavicarpaDeg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL−16-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21±1.13) primordial from the nodal meristems. Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1of each BAP and Kinetin (Kin) was successful for the multiplication of the shootsin vitrowith maximum numbers of shoots (25.73±0.06) within four weeks of incubation. Shoots were rooted best (7.13±0.56roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1indole-3 butyric acid (IBA). Allin vitroregenerated shoots were rooted byex vitromethod, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1IBA. The plantlets were hardened in the greenhouse for 4-5 weeks. The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.

scholarly journals PLANT REGENERATION OF AVOCADO (PERSEA AMERICANA MILL) IN VITRO

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696d-696
Author(s):  
Yaseen Mohamed-Yaseen ◽  
Raymond J. Schnell ◽  
Robert J. Knight ◽  
T.L. Davenport
Keyword(s):  
Tissue Culture ◽  
Multiple Shoots ◽  
Persea Americana ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Embryonic Axes ◽  
Phenolic Oxidation

A procedure was developed to regenerate plants via tissue culture from embryonic axes of mature avocado seeds. Explants were cultured in Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and naphthalene-acetic acid (NAA) or thidiazuron (TDZ) and NAA. Culture were kept in the dark for 7-10 days to reduce browning resulting from phenolic oxidation. Multiple shoots (5-8) were formed after transfer to light. Further multiplication were achieved using different combination of BA and NAA or TDZ and NAA. Shoots were cultured in MS supplemented with 2mg/l indolebutyric acid (IBA) for 2 weeks then transferred to MS supplemented with lg/l activated charcoal for root induction. Complete plants were obtained in vitro.

scholarly journals In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.

2020 ◽  
Vol 66 (4) ◽  
pp. 25-31
Author(s):  
Magdalena Tomaszewska-Sowa
Keyword(s):  
Leaf Area ◽  
Growth Regulators ◽  
Clonal Propagation ◽  
Shoot Length ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation ◽  
Number Of Leaves ◽  
In Vitro Clonal Propagation

Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.

scholarly journals Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

2018 ◽  
Vol 12 (2) ◽  
pp. 405-415
Author(s):  
Paulo Mauricio Centenaro Bueno ◽  
Luiz Antonio Biasi ◽  
Mauro Brasil Dias Tofanelli
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Simple Protocol ◽  
Growth Room ◽  
In Vitro Shoot Proliferation

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

Micropropagation of two threatened Tasmanian species of Calocephalus (Asteraceae), with comments on phenotypic plasticity

2003 ◽  
Vol 51 (4) ◽  
pp. 415 ◽  
Author(s):  
Rebecca Jane Sands ◽  
Natalie Ruth Brown ◽  
Anthony Koutoulis
Keyword(s):  
Phenotypic Plasticity ◽  
Plant Growth Regulator ◽  
Root Formation ◽  
Indoleacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Ex Vitro ◽  
Morphological Differences

Micropropagation systems were developed for Calocephalus citreus Less. and C. lacteus Less., two threatened Tasmanian members of the Asteraceae. Disinfected cold-treated capitula were used to initiate regeneration. For C. citreus, initiation was achieved on Murashige and Skoog (MS) medium with 0.1�mg�L–1 or 0.5�mg�L–1 indoleacetic acid (IAA) and 1�mg�L–1 6-benzylaminopurine (BAP) in 5�weeks, while for C. lacteus initiation was achieved on MS with α-naphthaleneacetic acid (NAA) (0.1�mg�L–1) in 3�weeks and on MS without any plant growth regulator (PGR) in 6�weeks. Multiplication was achieved in both species on MS with various concentrations of IAA (0.01–0.5�mg�L–1) and BAP (0.1–1�mg�L–1). In C. citreus, shooting in all treatments did not differ significantly from PGR-free MS, while in C. lacteus PGR-free MS was one of the better treatments. Multiplication media also initiated root formation in C. lacteus, thereby facilitating immediate planting out. Optimal root induction in C. citreus was achieved by using MS with 1�g�L–1 activated charcoal. Clear morphological differences between in vitro and ex vitro plants of both species were observed. This phenotypic plasticity was more pronounced in C. lacteus than in C. citreus. As C. lacteus has a wider distribution than C. citreus and C. lacteus was more responsive during many stages of the micropropagation process, it may be possible to use the culture-induced phenotype to provide insights into the ecology of plant species.

scholarly journals In vitro regeneration for mass propagation in commercial scale of medicinal plant vitex nigundo L.

1970 ◽  
Vol 18 ◽  
pp. 140-145 ◽  
Author(s):  
Md Abu Hena Mostofa Jamal ◽  
ANM Rubaiyath-Bin Rahman ◽  
Dipak Kumar Paul ◽  
Md Rezuanul Islam
Keyword(s):  
Survival Rate ◽  
Medicinal Plant ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Commercial Scale

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials and Methods: Shoot tips and nodal segments were used for multiple shoot induction. All explants were cultured on MS medium supplemented with various plant growth regulators. HgCl2 was used as surface sterilizing agent. For in vitro rooting, individual shoots (3-4 cm) were cut from the proliferated shoot cultures and implanted on half and full strength of MS with different concentrations and combinations of NAA and IAA. The cultures were incubated for 16 h photoperiod at 25 ± 2ºC under a fluorescent light. Visual observation of culture was made every week. Data on shoot induction and proliferation and root induction were recorded after three weeks of inoculation and used for calculation. For each treatment 15 explants were used and all the treatments were repeated thrice. Established plantlets were transplanted in earthen pots under natural conditions and the survival rate was recorded. Results: The most effective surface sterilization treatment has been found 0.1 % HgCl2 for 7 minutes. Highest number of shoot was observed in MS medium supplemented with 3.0 mg/ BAP. It was rooted well in full MS containing 2.0 mg/l IAA. The survival rate was 85 % and propagated plantlets were successfully acclimatized in soil. Conclusion: It was observed that shoot tips are more responsive for micropropagation of Vitex nigundo L . Thus the fruitful utilization of rapid clonal propagation, germplasm conservation and distribution of Vitex nigundo, important medicinal plant of Bangladesh, is possible. Keywords: Vitex nigundo; Medicinal plant; Shoot induction; Micropropagation; Regeneration. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8790 JBS 2010; 18(0): 140-145

scholarly journals Establishment of Cell Suspension Culture and Plant Regeneration in Abrus precatorius L., a Rare Medicinal Plant

2012 ◽  
Vol 4 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Mohammad Serajur RAHMAN ◽  
Mohammad Abdul Bari MIAH ◽  
Mohammad Shahadat HOSSAIN ◽  
Ahmad Humayan KABIR ◽  
Mohammad Motiur RAHMAN
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Liquid Media ◽  
Indolebutyric Acid ◽  
Abrus Precatorius ◽  
Isolated Cell

A new protocol has been developed for cell culture and in vitro regeneration of Abrus precatorius that holds enormous potentiality for preparation of medicines. In vitro grown calli were cultured in Murashige and Skoog (MS) liquid media in agitated condition fortified with 0.5 mg/l 6-Benzylaminopurine. Growth curve of cells revealed that the cells continued to grow until 12 days of culture and got the highest peak from day 6-8. Isolated cell was found to produce highest 8.2% calli when suspended on MS medium supplemented with 0.5 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. Callus derived from single cell produced highest number of embryo (25-28%) cultured on MS medium fortified with 2.0 mg/l 6-Benzylaminopurine and 0.2 mg/l 1-Naphthaleneacetic acid. The bipolar embryos were selected and optimum shoot formation was recorded on MS medium supplemented with 2.0 mg/l 6-Benzylaminopurine and 0.1 mg/l 1-Naphthaleneacetic acid. The optimum root induction was noticed in MS medium supplemented with 1.0 mg/l 3-Indolebutyric acid. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.

scholarly journals Adventitious Bud Induction and Plant Regeneration from Stem Nodes of Salvia splendens ‘Cailinghong’

HortScience ◽  
2015 ◽  
Vol 50 (6) ◽  
pp. 869-872
Author(s):  
Ling Yu ◽  
Hongwei Chen ◽  
Peipei Hong ◽  
Hongli Wang ◽  
Kefeng Liu
Keyword(s):  
Survival Rate ◽  
Naphthalene Acetic Acid ◽  
Adventitious Bud ◽  
Root Induction ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Salvia Splendens ◽  
Bud Induction ◽  
Bedding Plant ◽  
Adventitious Bud Induction

Salvia splendens is a widely used ornamental bedding plant; however, the limited propagation method has decreased its quality and yield. Through years of selection, we have obtained a new variety of S. splendens with weak apical dominance and named it as ‘Cailinghong’. To establish an effective method for regeneration of S. splendens ‘Cailinghong’, different explants, including leaves, receptacles, petioles, stem nodes, and stem segments were used for adventitious bud induction. Next, various combinations of plant growth regulators (PGRs) were selected for bud and root induction, which were assessed by adventitious bud initiation rate and proliferation rate, as well as root induction rate. Meanwhile, the survival rate of transplanted plantlets was also calculated. As a result, stem nodes were found easy to be induced to form buds, and the optimum medium component was 1/2 Murashige and Skoog (MS) medium supplemented with 0.45 µM naphthalene acetic acid (NAA), 8.88 µM 6-benzylaminopurine (6-BA), and 2.46 µM 3-indolebutyric acid (IBA) for plantlets induction, whereas 1/4 MS medium supplemented with 2.23 µM NAA for root induction. Furthermore, the survival rate of transplanted plantlets was up to 80%, and all regenerated plantlets were normal in phenotype. Therefore, cultured in 1/2 MS medium with combined PGRs, whole plantlet of S. splendens ‘Cailinghong’ could be regenerated directly from stem node.

scholarly journals Clonal propagation of Carob (Ceratonia siliqua L., Fabaceae)

1970 ◽  
Vol 39 (1) ◽  
pp. 15-19 ◽  
Author(s):  
L Hakim ◽  
MR Islam ◽  
ANK Mamun ◽  
G Ahmed ◽  
R Khan
Keyword(s):  
Clonal Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Root Induction ◽  
Ms Medium ◽  
Ceratonia Siliqua ◽  
Moderate Amount ◽  
Half Strength

Mature seeds of carob tree (Ceratonia siliqua L.) were germinated on hormone free MS medium. Efforts were made to develop multiple shoots by using axillary buds of in vitro grown seedlings on MS medium fortified with different concentrations of BA singly and BA in combination with IAA or GA3. Axillary buds produced single shoot with a moderate amount of callus at the base of the explant after culturing on MS medium with BA alone. Multiple shoots were regenerated when explants when cultured on MS medium fortified with BA + IAA or BA + GA3. MS medium supplemented with 1.5 mg/l BA + 0.5 mg/l GA3 was found more effective in multiple shoot regeneration than all other combinations. Regenerated multiple shoots were excised and cultured on half strength of MS medium containing different concentrations of IBA for root induction. Best root development was obtained in half strength MS medium containing 0.5 mg/l IBA. About 70% of the regenerated plantlets survived in natural conditions. Key words: Carob; Ceratonia siliqua; Fabaceae; Clonal propagation DOI: 10.3329/bjb.v39i1.5520Bangladesh J. Bot. 39(1): 15-19, 2010 (June)

scholarly journals Improved Rooting and Acclimatization of Micropropagated Hazelnut Shoots

HortScience ◽  
2004 ◽  
Vol 39 (7) ◽  
pp. 1688-1690 ◽  
Author(s):  
Mehmet Nuri Nas ◽  
Paul E. Read
Keyword(s):  
Survival Rate ◽  
Plant Growth ◽  
Butyric Acid ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Root Induction ◽  
Ex Vitro ◽  
Potential Use ◽  
Commercial Micropropagation

Microshoots of four hazelnut genotypes grown in vitro on Nas and Read medium (NRM) containing various combinations of CuSO4 • 5H2O and myo-inositol were successfully rooted and acclimatized ex vitro without any need of in vitro hardening treatments. Dipping of shoot bases in 1000 ppm indole-3-butyric acid (IBA) solution for 5 or 10 seconds followed by placement of shoots in plant growth regulator free NRM gave rise to formation of roots as early as 8 days. Shoots treated for 5 and 10 seconds rooted similarly, and depending on genotype, 88% to 98% rooting was observed within 15 days after treatment with IBA. Ex vitro survival of shoots three months after in vitro-root induction was 73% when shoots were treated with IBA for 5 seconds and 66% when shoots were treated for 10 seconds. The highest ex vitro survival rate (97%) 3 months after root induction was observed when shoots were treated with IBA solution for 10 seconds, and then cultured directly in peat pellets. Shoots developed good roots, and grew up to 70 cm in height 3 months after root induction. The potential use of rooting and acclimatization protocol for commercial micropropagation of hazelnut is presented.

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