scholarly journals AE804, AE806, AE999, AF017, AF041 and AF131 antibodies label mouse insulin-secreting beta cells by immunohistochemistry

2020 ◽  
Vol 3 (1) ◽  
pp. e125
Author(s):  
Alessia D’Esposito ◽  
Cyril Guilhen ◽  
Priscilla Soulié
Keyword(s):  
Pancreatic Islets ◽  
Beta Cells ◽  
Recombinant Antibodies ◽  
Mouse Pancreatic Islets

The recombinant antibodies AE804, AE806, AE999, AF017, AF041 and AF131 detect by immunohistochemistry the insulin-secreting beta cells in mouse pancreatic islets. The most efficient staining was obtained with AE999.

scholarly journals Inosine-stimulated insulin release and metabolism of inosine in isolated mouse pancreatic islets

1976 ◽  
Vol 158 (2) ◽  
pp. 335-340 ◽  
Author(s):  
K Capito ◽  
C J Hedeskov
Keyword(s):  
Insulin Secretion ◽  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Pancreatic Islets ◽  
Beta Cells ◽  
Insulin Release ◽  
Lactate Production ◽  
Islet Metabolism ◽  
Mouse Pancreatic Islets ◽  
Mouse Islets

Inosine is a potent primary stimulus of insulin secretion from isolated mouse islets. The inosine-induced insulin secretion was totally depressed during starvation, but was completely restored by the addition of 5 mM-caffeine to the medium and partially restored by the addition of 5 mM-glucose. Mannoheptulose (3 mg/ml) potentiated the effect of 10 mM-inosine in islets from fed mice. The mechanism of the stimulatory effect of inosine was further investigated, and it was demonstrated that pancreatic islets contain a nucleoside phosphorylase capable of converting inosine into hypoxanthine and ribose 1-phosphate. Inosine at 10 mM concentration increased the lactate production and the content of ATP, glucose 6-phosphate (fructose 1,6-diphosphate + triose phosphates) and cyclic AMP in islets from fed mice. In islets from starved mice inosine-induced lactate production was decreased and no change in the concentration of cyclic AMP could be demonstrated, whereas the concentration of ATP and glucose 6-phosphate rose. Inosine (10 mM) induced a higher concentration of (fructose 1,6-diphosphate + triose phosphates) in islets from starved mice than in islets from fed mice suggesting that in starvation the activities of glyceraldehyde 3-phosphate dehydrogenase or other enzymes below this step in glycolysis are decreased. Formation of glucose from inosine was negligible. Inosine had no direct effect on adenylate cyclase activity in islet homogenates. The observed changes in insulin secretion and islet metabolism mimic what is seen when glucose and glyceraldehyde stimulate insulin secretion, and as neither ribose nor hypoxanthine-stimulated insulin release, the results are interpreted as supporting the substrate-site hypothesis for glucose-induced insulin secretion according to which glucose has to be metabolized in the beta-cells before secretion is initiated.

scholarly journals AK247, AK249, AK250, AK280 and AK281 antibodies label mouse glucagon-secreting alpha cells by immunohistochemistry

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Cyril Guilhen ◽  
Ezia Oppliger ◽  
Marie N. Schmid ◽  
Clément Bindschaedler ◽  
Margaux Gosetto ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Recombinant Antibodies ◽  
Alpha Cells ◽  
Mouse Pancreatic Islets

The recombinant antibodies AK247, AK249, AK250, AK280 and AK281 detect by immunohistochemistry the glucagon-secreting alpha cells in mouse pancreatic islets.

scholarly journals Adaptations of alpha2- and beta-cells of rat and mouse pancreatic islets to starvation, to refeeding after starvation, and to obesity.

1980 ◽  
Vol 65 (1) ◽  
pp. 207-218 ◽  
Author(s):  
F M Matschinsky ◽  
C Rujanavech ◽  
A Pagliara ◽  
W T Norfleet
Keyword(s):  
Pancreatic Islets ◽  
Beta Cells ◽  
Mouse Pancreatic Islets

Phosphofructokinase isozymes in pancreatic islets and clonal beta-cells (INS-1)

Diabetes ◽  
1995 ◽  
Vol 44 (11) ◽  
pp. 1285-1289 ◽  
Author(s):  
G. C. Yaney ◽  
V. Schultz ◽  
B. A. Cunningham ◽  
G. A. Dunaway ◽  
B. E. Corkey ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Beta Cells

Slow [Ca2+]i oscillations induced by ketoisocaproate in single mouse pancreatic islets

Diabetes ◽  
1995 ◽  
Vol 44 (3) ◽  
pp. 300-305 ◽  
Author(s):  
F. Martin ◽  
J. V. Sanchez-Andres ◽  
B. Soria
Keyword(s):  
Pancreatic Islets ◽  
Mouse Pancreatic Islets

Adenosine uptake by isolated mouse pancreatic islets

1981 ◽  
Vol 30 (15) ◽  
pp. 2075-2080 ◽  
Author(s):  
Michael Welsh ◽  
Arne Andersson
Keyword(s):  
Pancreatic Islets ◽  
Adenosine Uptake ◽  
Mouse Pancreatic Islets
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