The AE889 and AI500 antibodies recognize Klebsiella pneumoniae surface antigens by flow cytometry

The recombinant antibodies AE889 and AI500 bind to the surface of the K. pneumoniae 52145 strain as detected by flow cytometry; AI144, AI501, AI502, AI505 and AS733 antibodies do not.

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Use of Allophycocyanin Allows Quantitative Description by Flow Cytometry of Alveolar Macrophage Surface Antigens Present in Low Numbers of Cells

American Review of Respiratory Disease ◽

10.1164/ajrccm/138.5.1124 ◽

1988 ◽

Vol 138 (5) ◽

pp. 1124-1128 ◽

Cited By ~ 13

Author(s):

Henry J. Fuchs ◽

Joan McDowell ◽

Judd E. Shellito

Keyword(s):

Flow Cytometry ◽

Alveolar Macrophage ◽

Quantitative Description ◽

Surface Antigens

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Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry

Methods in Molecular Biology - Monoclonal Antibodies ◽

10.1007/978-1-62703-992-5_6 ◽

2014 ◽

pp. 81-103

Author(s):

Alejandro Uribe-Benninghoff ◽

Teresa Cabral ◽

Efthalia Chronopoulou ◽

Jody D. Berry ◽

Cindi R. Corbett

Keyword(s):

Flow Cytometry ◽

Cell Surface ◽

High Throughput ◽

Surface Antigens ◽

Cell Surface Antigens ◽

Homogeneous Assay

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The Association of High Prevalence of Trophozoites in Peripheral Blood with Lower Antibody Response toP. falciparumInfected Erythrocytes among Asymptomatic Children in Sudan

Mediators of Inflammation ◽

10.1155/2016/7987686 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4

Author(s):

Sara N. Mohamed ◽

Dina A. Hassan ◽

Abdelrahim M. El Hussein ◽

Ihssan M. Osman ◽

Muntasir E. Ibrahim ◽

...

Keyword(s):

Flow Cytometry ◽

Plasmodium Falciparum ◽

Peripheral Blood ◽

Surface Antigens ◽

Igg Antibodies ◽

Autologous Plasma ◽

Variant Surface Antigens ◽

Asymptomatic Children ◽

Infected Rbcs ◽

The Mean

Background. The most prominent variant surface antigens (VSAs) ofPlasmodium falciparumare the var gene-encodedPlasmodium falciparumerythrocyte membrane protein 1 (PfEMP1) family, which serves as a parasite-sequestering ligand to endothelial cells. In this study we have examined the antibody reactivity of autologous plasma from symptomatic and asymptomatic malaria infected children against the infected erythrocytes’ surface antigens using flow cytometry.Methods. Ethidium-bromide-labelled erythrocytic mature forms ofP. falciparumparasites obtained from symptomatic and asymptomatic children were sequentially incubated with autologous plasma and fluorescein isothiocyanate-conjugated (FITC) antihuman IgG. Plasma antibody reactivity was detected by flow cytometry.Results. Asymptomatic children had more prevalence of trophozoites in peripheral blood (66%) compared to symptomatic children (16%),p=0.002. The mean percentage of infected RBCs reacting with autologous sera was 89.78 among symptomatic children compared to 79.62 among asymptomatic children (p=0.09). Moreover, the mean fluorescence intensity (MFI) in the asymptomatic was significantly higher compared to symptomatic children (pvalue = 0.040).Conclusion. Variant surface antigens onPlasmodium falciparuminfected RBCs from symptomatic malaria children tend to be better recognized by IgG antibodies. This may suggest a role of some IgG antibodies in severity of malaria.

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The malignant cells in a Lennert's lymphoma are T lymphocytes with a mature helper surface phenotype. A multiparameter flow cytometric analysis

Blood ◽

10.1182/blood.v68.2.426.426 ◽

1986 ◽

Vol 68 (2) ◽

pp. 426-429 ◽

Cited By ~ 7

Author(s):

KJ Stonesifer ◽

NA Benson ◽

SE Ryden ◽

DF Pawliger ◽

RC Braylan

Keyword(s):

Flow Cytometry ◽

Flow Cytometric Analysis ◽

Malignant Neoplasm ◽

Surface Antigens ◽

Light Scatter ◽

Malignant Cells ◽

T Helper ◽

T Helper Lymphocytes ◽

Flow Cytometric ◽

Multiparameter Analysis

Abstract The flow cytometric analysis of DNA content in cells obtained from a case of Lennert's lymphoma demonstrated the presence of a discrete hypotetraploid cell population. Correlated multiparameter analysis of DNA, light scatter, and surface antigens by flow cytometry showed that the hypotetraploid cells were intermediate to large cells expressing T11, T3, and T4 antigens and lacking B1 and T8 antigens. These findings suggest that Lennert's lymphoma represents a malignant neoplasm of T- helper lymphocytes.

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Signal amplification in flow cytometry for cell surface antigen analysis

The Journal of Biochemistry ◽

10.1093/jb/mvz052 ◽

2019 ◽

Vol 166 (3) ◽

pp. 205-212 ◽

Cited By ~ 2

Author(s):

Takeshi Mori ◽

Yoshiki Katayama

Keyword(s):

Flow Cytometry ◽

Cell Surface ◽

Surface Antigen ◽

Signal Amplification ◽

Surface Antigens ◽

Antibody Activity ◽

Cell Surface Antigens ◽

Promising Strategy ◽

Fluorescent Polymer ◽

Enzymatic Signal Amplification

AbstractSignal enhancing systems have been introduced to enable detection of cell surface antigens by flow cytometry. Cell surface antigens are important targets that describe the function and lineage of cells. Although flow cytometry is an effective tool for analysing cell surface antigens, this technique has poor sensitivity, which prohibits the detection of many important antigens on cell membranes. Thus, signal amplification is essential for developing practical tools for evaluating cell surface antigens by flow cytometry. Using a bright fluorophore or fluorescent polymer incorporated into antibodies is a straightforward strategy to improve flow cytometry sensitivity but may affect the functional characteristics of the labelled antibody. In contrast, enzymatic signal amplification is a more practical and efficient strategy to improve sensitivity that should not affect antibody activity. Although enzymatic signal amplification still has a number of drawbacks, this approach is a promising strategy to analyse cell surface antigens.

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