Rapid detection of recombinant antibody fragments directed against cell-surface antigens by flow cytometry

1996 ◽  
Vol 196 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Sergey M. Kipriyanov ◽  
Olga A. Kupriyanova ◽  
Melvyn Little ◽  
Gerhard Moldenhauer
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
Rapid Detection ◽  
Recombinant Antibody ◽  
Surface Antigens ◽  
Antibody Fragments ◽  
Cell Surface Antigens ◽  
Recombinant Antibody Fragments

Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry

2014 ◽  
pp. 81-103
Author(s):  
Alejandro Uribe-Benninghoff ◽  
Teresa Cabral ◽  
Efthalia Chronopoulou ◽  
Jody D. Berry ◽  
Cindi R. Corbett
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
High Throughput ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Homogeneous Assay

scholarly journals Signal amplification in flow cytometry for cell surface antigen analysis

2019 ◽  
Vol 166 (3) ◽  
pp. 205-212 ◽  
Author(s):  
Takeshi Mori ◽  
Yoshiki Katayama
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
Surface Antigen ◽  
Signal Amplification ◽  
Surface Antigens ◽  
Antibody Activity ◽  
Cell Surface Antigens ◽  
Promising Strategy ◽  
Fluorescent Polymer ◽  
Enzymatic Signal Amplification

AbstractSignal enhancing systems have been introduced to enable detection of cell surface antigens by flow cytometry. Cell surface antigens are important targets that describe the function and lineage of cells. Although flow cytometry is an effective tool for analysing cell surface antigens, this technique has poor sensitivity, which prohibits the detection of many important antigens on cell membranes. Thus, signal amplification is essential for developing practical tools for evaluating cell surface antigens by flow cytometry. Using a bright fluorophore or fluorescent polymer incorporated into antibodies is a straightforward strategy to improve flow cytometry sensitivity but may affect the functional characteristics of the labelled antibody. In contrast, enzymatic signal amplification is a more practical and efficient strategy to improve sensitivity that should not affect antibody activity. Although enzymatic signal amplification still has a number of drawbacks, this approach is a promising strategy to analyse cell surface antigens.

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