Staphylococcus spp. from wild mammals in Aragón (Spain): antibiotic resistance status

AbstractIntroductionAntimicrobial resistance is a global health threat. It has been studied in humans and domestic animals, but there is a lack of data on wild animals. The objective of this study is the elucidation of its patterns in Staphylococcus spp. isolated from wild mammals of the Autonomous Community of Aragón (Spain).Material and MethodsA total of 103 mammals (Artiodactyla, Carnivora, Chiroptera, Erinaceomorpha, and Lagomorpha) were studied. A recovery centre provided 32 and hunting 71. Nasal and faecal samples yielded 111 staphylococci, which were identified by matrix-assisted laser desorption/ionization–time of flight mass spectrometry. A susceptibility test to 11 antibiotics was carried out, and statistical analysis was performed.ResultsSome differences were detected in bacterial prevalence depending on how the mammal fed. Artiodactyla, mainly hunted, were predisposed to carry coagulase-positive staphylococci. The staphylococci species recovered were resistant to at least two classes of antibiotics, and were disseminated in all of the geographical areas studied.ConclusionResistant staphylococci are widely distributed in the wild mammals in the areas of the study, but the resistance quantified in them is lower than that to be expected if the use of antibiotics in farms had a direct influence on the wildlife and its environment. On the other hand, resistance to antibiotics restricted to human use was widely disseminated in various wild animal species.

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Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR

Veterinary Microbiology ◽

10.1016/j.vetmic.2017.03.036 ◽

2017 ◽

Vol 204 ◽

pp. 8-14 ◽

Cited By ~ 8

Author(s):

Lucas J.L. Pizauro ◽

Camila C. de Almeida ◽

Glenn A. Soltes ◽

Durda Slavic ◽

Oswaldo D. Rossi-Junior ◽

...

Keyword(s):

Laser Desorption ◽

Species Level ◽

Coagulase Negative Staphylococcus ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Real Time Quantitative Pcr ◽

Flight Mass Spectrometry ◽

Staphylococcus Spp ◽

Level Identification ◽

Matrix Assisted Laser

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Aus der § 64 LFGB-Arbeitsgruppe MALDI-TOF: Leitlinien für die Validierung von Spezies-Identifizierungen mittels MALDI-TOF-MS

Journal of Consumer Protection and Food Safety ◽

10.1007/s00003-021-01353-x ◽

2021 ◽

Author(s):

Jörg Rau ◽

Tobias Eisenberg ◽

Christine Wind ◽

Ingrid Huber ◽

Melanie Pavlovic ◽

...

Keyword(s):

Animal Species ◽

Laser Desorption ◽

Laser Desorption Ionization ◽

Ionization Time ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Flight Mass Spectrometry ◽

Reference Databases ◽

New Applications ◽

Tof Ms

AbstractMatrix-assisted laser-desorption/ionization-time-of-flight-mass-spectrometry (MALDI-TOF-MS) is widely used to identify microorganisms. Recently, new applications such as identification of the animal species from meat, milk or fish are emerging. Standards for the validation of species identifications are still missing. Now, the § 64-LFGB working-group “MALDI-TOF”, established at the Federal Office of Consumer Protection and Food Safety, has compiled a guideline for the validation of species identifications. This guideline is intended for single laboratories as well as for lab networks and shows practical ways for validation of qualitative MALDI-TOF-MS methods. The special opportunities of the technology, in particular the use of extended reference databases and of collections of well-documented individual spectra for validation, have been taken into account in the guideline presented.

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Mycobacteria fecal shedding in wild boars (Sus scrofa), South-eastern France

10.1101/670166 ◽

2019 ◽

Author(s):

Mustapha Fellag ◽

Michel Drancourt ◽

Jean-Lou Marié ◽

Bernard Davoust

Keyword(s):

Wild Boar ◽

Nontuberculous Mycobacteria ◽

Laser Desorption Ionization ◽

Fecal Matter ◽

Ionization Time ◽

Fecal Shedding ◽

Wild Boars ◽

Flight Mass Spectrometry ◽

In The Wild ◽

Species Being

ABSTRACTThe recent recrudescence of tuberculosis in cattle has implicated wild boar as a reservoir and vector of this disease, which led to the investigation of fecal shedding of the causativeMycobacterium bovis. In the Provence region of France, wild boars are very abundant, and the current study was carried out to assess the presence of tuberculous and nontuberculous mycobacteria in feces of wild boar population. W e developed an original protocol allowing the fast isolation of mycobacteria by combining a 1%-chlorhexidine decontamination of fecal matter with culture on MOD9 medium. Colonies were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, combined with DNA sequencing. This protocol yielded no tuberculous mycobacteria among ninety-nine wild boar fecal samples collected in the Provence region. However, non-tuberculous mycobacteria were isolated from five samples (5.05%), includingMycobacterium peregrinum, Mycobacterium vaccaeandMycobacterium setense, the last species being previously unreported in the wild boar; in addition to two positive samples forNocardiaspp. In conclusion, wild boars in southeastern France are not shedding tuberculosis agents, but they could constitute a reservoir of human non-tuberculous mycobacteriosis in selected populations directly exposed to wild boars.

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