Susceptibility of naked oat cultivars to seed sprouting

2014 ◽  
Vol 9 (8) ◽  
pp. 823-832
Author(s):  
Andrzej Zieliński ◽  
Magdalena Simlat ◽  
Tomasz Wójtowicz ◽  
Maria Moś
Keyword(s):  
Abscisic Acid ◽  
Gibberellic Acid ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Falling Number ◽  
Total Rainfall ◽  
Naked Oat ◽  
Germination Time ◽  
Average Decrease ◽  
Oat Cultivars

AbstractThe production of economically important cereals is accompanied by the phenomenon of sprouting which in naked cultivars may limit their reproduction and usability. The objective of the work is to evaluate the susceptibility to sprouting in naked oat cultivars, and to test the usefulness of sprouting indices. In the years 2008–2010 for seeds of 8 cultivars, differing in the degree of sprouting damage, the coefficient of sprouting (Cs) was determined. Germinability (GF), dynamics (GD) and average germination time (GAT) were determined for seeds germinating in the presence of abscisic acid (ABA), gibberellic acid (GA3) and under control conditions. Basing on the falling number (FN) in consecutive days of the sprouting induction, alpha-amylase activity was determined. The highest values of Cs were found in 2008, the year with the highest total rainfall and temperature. In the presence of ABA the GF decreased by 21%, the GAT was 4.7 days longer, and the GD decreased by 55% compared with other substrates. An increase in alpha-amylase activity contributed to a 50%, on average, decrease in FN at 10°C and 30°C after 48 and 24 h of incubation, respectively. In the analyzed years the greatest resistance to sprouting was found for Bullion seeds.

Alpha-amylase activity of wheat grain from crops differing in grain drying rate

1997 ◽  
Vol 128 (2) ◽  
pp. 127-134 ◽  
Author(s):  
P. S. KETTLEWELL ◽  
M. M. CASHMAN
Keyword(s):  
Amylase Activity ◽  
Potential Evapotranspiration ◽  
Analysis Of Covariance ◽  
Alpha Amylase ◽  
Drying Rate ◽  
Falling Number ◽  
Seasonal Differences ◽  
Year Effect ◽  
Grain Drying ◽  
Hagberg Falling Number

The hypothesis was tested that slow grain drying stimulates pre-maturity alpha-amylase activity in wheat (Triticum aestivum L.). Grain drying rate in 91 commercial crops of cultivars Avalon or Mercia grown over the years 1988–90 was estimated from the slope of linear regressions of moisture content on time. Incipient sprouting was detected in some samples from 1988 using the fluoroscein dibutyrate test, but results from a beta-limit dextrin gel assay indicated that pre-maturity alpha-amylase was probably the major source of alpha-amylase activity in these samples. Although year-to-year differences in ln alpha-amylase activity and Hagberg falling number tended to be associated with seasonal differences in drying rate, there was no evidence of a relationship between either ln alpha-amylase activity or Hagberg falling number and drying rate after the year effect was removed by an analysis of covariance. A second dataset from one crop in each of 14 years at one site showed significant positive linear relationships between cumulative potential evapotranspiration calculated over different periods during grain ripening and Hagberg falling number (excluding 3 years when incipient sprouting was thought to occur). It was concluded that pre-maturity alpha-amylase activity was stimulated by an unknown environmental factor differing between seasons and associated with seasonal differences in drying rate and cumulative potential evapotranspiration.

scholarly journals Action of gibberellic acid (GA3) on dormancy and activity of alpha-amylase in rice seeds

2002 ◽  
Vol 24 (2) ◽  
pp. 43-48 ◽  
Author(s):  
Antônio Rodrigues Vieira ◽  
Maria das Graças Guimarães Carvalho Vieira ◽  
Antônio C. Fraga ◽  
João Almir Oliveira ◽  
Custódio D. dos Santos
Keyword(s):  
Enzyme Activity ◽  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Rice Seed ◽  
Efficient Treatment ◽  
Soaking Time ◽  
Forced Air ◽  
Air Circulation

To evaluate the effectiveness of gibberellic acid (GA3) in breaking rice seed dormancy and the use of alpha-amylase enzyme activity as an indicator of the dormancy level, seed from the intensively dormant irrigated cultivar Urucuia were used. The seeds were submitted to a pre-drying process in a forced air circulation chamber under 40ºC during 7 days and submersed in 30 mL of GA3 solution under 0, 10, 30 and 60 mg/L H2O concentrations, during 2, 24 and 36 hours. After the treatments, the alpha-amylase activity was determined by using the polyacrilamide electrophoresis and spectrophotometry. At the same time, the germination test was made. The results indicated a gain in germination and in alpha-amylase activity in higher concentrations and soaking time of seeds in GA3. These observations support the conclusion that soaking seed in 60 mg GA3/L during 36 hours can be used as a quick and efficient treatment in breaking rice seed dormancy and is equivalent to the forced air circulation chamber at 40ºC during 7 days. The alpha-amylase enzyme activity proved to be as an efficient marker of the seeds dormancy level.

Effects of spring nitrogen fertilizer on the Hagberg falling number of grain from breadmaking varieties of winter wheat

1986 ◽  
Vol 107 (2) ◽  
pp. 475-477 ◽  
Author(s):  
M. J. Gooding ◽  
P. S. Kettlewell ◽  
W. P. Davies ◽  
T. J. Hocking
Keyword(s):  
Nitrogen Fertilizer ◽  
European Economic Community ◽  
Amylase Activity ◽  
Quality Measurement ◽  
Fertilizer Application ◽  
Alpha Amylase ◽  
Nitrogen Application ◽  
Falling Number ◽  
Previous Suggestion ◽  
Hagberg Falling Number

Hagberg falling number (HFN) is used to estimate alpha-amylase activity in wheat grain (Perten, 1964) and it is utilized by flour millers and the Intervention Agencies of the European Economic Community as a grain quality measurement for breadmaking (Anon. 1983). High nitrogen fertilizer application can lead to lodging and can decrease HFN (Brun, 1982). This may result from damp conditions around the ear encouraging germination, and therefore increasing alpha-amylase activity (Stewart, 1984). However, since nitrogen application can also delay maturity, it has been suggested that this influence could maintain high falling number (Anon. 1985). Pushman & Bingham (1976) found that increased nitrogen application decreased alpha-amylase activity, lending support to the previous suggestion, but contrasting with the results of Brun (1982).

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